Fast Polymerization for Quick Sample-to-Answer Time
The average time-to-result (TTR) for Lyo-Ready ™ LAMP Mix ( orange ) and a mix from supplier N ( blue ) were compared for three genes, 9TV10 from Trichomonas Vaginalis (100 copies), hBRCA1 from human genomic DNA (200 copies) and MTB1 from Mycobacterium Tuberculosis (1,000 copies). The results demonstrate the faster speed of Lyo-Ready LAMP Mix, with earlier TTR values when compared to supplier N. Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
25.00
20.00
15.00
10.00
5.00
0.00
9TV10 Trichomonas vaginalis
hBRCA-1
MTB1
Human DNA
Mycobacterium tuberculosis
MDX097 | Supplier N
The average time to results (TTR) for Lyo-Ready LAMP Mix ( orange ) and a mix from supplier O ( tan ) and supplier N ( blue ) were compared using a 10-fold serial dilution for the of BRCA1 gene (1,000, 100 and 10 copies). The relative concentrations of LAMP oligos were optimized to obtain earlier TTR compared to other experiments. The results demonstrate the increased sensitivity and greater reproducibility of the Lyo-Ready LAMP Mix, with earlier TTR values and a lower SD when compared to other suppliers. Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Better Sensitivity with Lower Sample Input
20
15
10
5
0
1,000 copies
100 copies
10 copies
MDX097 | Supplier O | Supplier N
Lyophilized mix maintains its shelf-life
LAMP reactions were performed using the fresh Lyo-Ready LAMP Mix and compared against lyophilized Lyo-Ready LAMP Mix, stored at 37°C for 3 months. The results show that the lyophilization process does not affect the stability of the Lyo-Ready LAMP Mix and with a temperature of 37°C we can extrapolate the stability of the lyophilized mix for up to 1 year. Reactions were incubated at 65°C for 60 min and TTR values were measured at 1:10 of end fluorescence.
10.00 8.00 12.00 14.00 20.00 18.00 16.00
0.00 2.00 6.00 4.00
9TV10 Trichomonas vaginalis
hBRCA-1
MTB1
Human DNA
Mycobacterium tuberculosis
Wet | Dry
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