Specimen-specific ™ qPCR & RT-qPCR Mixes
Blood-Specific Mixes
Blood
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer (liquid biopsy), cardiovascular disease, and metabolic disorders including diabetes. However, there are several PCR inhibitors such as immunoglobulin G, hemoglobin, lactoferrin and leukocyte DNA that are present in blood which impact a reaction’s efficiency. In addition, anticoagulants used to stabilize blood samples (e.g., EDTA, citrate or heparin) contain a number of inhibitors which cause interference in a PCR reaction. Over the past decade, many diagnostic assays that use blood as a specimen have improved in terms of turnaround time, sensitivity and specificity as traditional techniques and biomarkers have been replaced by newer, faster and more sensitive methods. The traditional approaches to overcoming inhibition however have relied on removing inhibitors through DNA or RNA extraction prior to testing, but these methods are not 100% effective, cause sample loss, and reduce reproducibility.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Blood Mix, 4x
MDX092
Air-Dryable Direct RNA/ DNA qPCR Blood Mix, 4x
MDX121
Lyo-Ready Direct DNA qPCR Blood Mixes
MDX122
Lyo-Ready Direct RNA/DNA qPCR Blood Mixes
MDX123
Lyo-Ready Genotyping Direct qPCR Blood
MDX128
Meridian’s Air-Dryable TM and Lyo-Ready TM direct blood mixes are unique by enabling direct amplification of target DNA or RNA from high concentrations of crudely processed blood, serum or plasma samples. They have been designed to efficiently amplify in the presence of inhibitors found in blood – no further optimization is required. Furthermore, these mixes can be used in a wet format or lyophilized to create ambient-temperature stable assays. High tolerance to whole blood stabilized with anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)
A) Air-Dryable™ Direct DNA qPCR Blood (MDX092)
B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121)
QuantaBio
TaqPath™ 1-Step
KAPA Probe Force
TaqPath™
K2-EDTA | Sodium heparin | Sodium citrate
Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. 0% 2%, 5% and 20% human whole blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) and sodium citrate ( grey ) was tested with (A) Air-Dryable TM Direct DNA qPCR Blood (MDX092) against mixes from KAPA Probe Force (Roche) and TaqPath™ (Thermo) and (B) Air-Dryable TM Direct RNA/DNA qPCR Blood (MDX121) against mixes from Ultraplex™ (QuantaBio) and TaqPath™ 1-Step (Thermo). The results demonstrate that the reaction efficiencies of the Air-Dryable TM Direct Blood mixes are higher both in the presence of anticoagulants and higher concentrations of blood than of other suppliers’ mixes.
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