Product Highlights
Enzyme maintains stability after freeze-thaw cycles
110
100
Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to protect Taq polymerase during freezing conditions. To test the resistance of Glycerol‑Free Taq Hot‑Start to freezing and thawing, a freeze‑thaw test was performed (10 and 15 cycles of freeze/thawing) and compared to fresh product (0 cycles of freeze/thawing). The results illustrate that Glycerol‑Free Taq Hot‑Start offers the same protection to freeze/thawing as a polymerase that contains glycerol.
90
80
70
60
50
0
10
15
F/T Cycles
Comparison of freshly prepared and lyophilized glycerol-free Taq HS master mixes
A 10-fold serial dilution of template DNA was used to set up two sets of qPCR assays. One set was lyophilized (blue) and the other left as a wet mix (black). Results illustrate that lyophilization of Glycerol-Free Taq HS does not have an effect on the quality of the qPCR, as both the assays demonstrate identical efficiency and sensitivity.
Ordering information: USA E: info@meridianlifescience.com Toll free: +1 800 327 6299 UK E: info.uk@meridianlifescience.com Tel: +44 (0)20 8830 5300
Germany E: info.de@meridianlifescience.com Tel: +49 (0)3371 60222 00 Australia E: info.au@meridianlifescience.com Tel: +61 (0)2 9209 4180
美国迈迪安生命科学公司 电子邮件: vivian.li@meridianlifescience.com 电话: +86-159-1103-0750
Connect with us:
www.MeridianLifeScience.com
ISO 13485 Certified
10/19
Powered by FlippingBook