Glycerol-Free T4 DNA Ligase
Glycerol-Free T4 DNA Ligase (HC) is a glycerol-free, high concentration enzyme (50 U/μL) that catalyzes the formation of a phosphodiester bond between adjacent 5’ phosphate and 3’ hydroxyl groups in duplex DNA or RNA. It is 2-10x higher in concentration than standard T4 DNA ligases and is glycerol-free and lyophilization compatible. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA/RNA hybrids.
Product Highlights • Efficiently joins blunt and cohesive ends • Repairs single-stranded nicks in duplex DNA, RNA and DNA/ RNA hybrids • Joins double-stranded oligonucleotide linkers or adaptors to DNA • Glycerol-free, suitable for lyophilization • Ideal for use in high-complexity library cloning and NGS library construction
PRODUCT
CAT NO.
VOLUME
20 μL (1,000 Weiss Units) 200 μL (10,000 Weiss Units)
Glycerol-Free T4 DNA Ligase (HC)
MDX200
100U T4 DNA Ligase
100U T4 DNA Ligase
No Ligase Control
100U T4 DNA Ligase
100U T4 DNA Ligase
No Ligase Control
Free of Detectable of Exo- and Endonuclease Activities
Glycerol-Free T4 DNA Ligase (HC) is highly purified and tested to ensure that it is free of any detectable exonuclease or endonuclease activity that may affect the ligation efficiency of the enzyme. It is also tested to ensure that there is no loss of activity during lyophilization and ambient temperature storage. The very high concentration makes Glycerol-Free T4 DNA Ligase (HC) ideal for using in cassettes for point of care (POC) assays. Glycerol-Free T4 DNA Ligase (HC) is free of detectable exo - and endonuclease activities. Glycerol-Free T4 DNA Ligase (HC) was incubated of for 16 hours at 37 °C with 1 µg of HindIII-digested λ DNA (Lanes 2 and 3) and supercoiled pUC19 DNA (Lanes 5 and 6). The results show no detectable exo- and endonuclease activities.
Glycerol-Free High-Fidelity Pfu (HC)
A high‑concentration (20 U/µL), glycerol‑free, high-fidelity thermostable DNA polymerase, a novel buffer system containing magnesium, dNTPs and excipients, to provide high yield and sensitivity, perfect for target enrichment, NGS library amplification, gene expression, cloning and site-directed mutagenesis.
Product Highlights • High concentration Pfu polymerase allowing for bulk production of PCR master mixes. • Ideal for NGS library amplification • Glycerol-free, ideal for preparation of dried-down PCR tests.
PRODUCT
CAT NO.
VOLUME
REACTION
0.1 mL
500 Rxns
Glycerol-Free High-Fidelity Pfu (HC)
MDX203
10 mL
5,000 Rxns
Multiplex amplification
Glycerol-Free High-Fidelity Pfu (HC), was lyophilized with Lyo-Ready™ Pfu Reaction Buffer, 5x and stored at 37°C for 7 and 28 days. Lyophilization samples were tested in multiplexed PCR amplification of 7 targets (793 bp, 649 bp, 548 bp, 418 bp, 332 bp, 196 bp, 135 bp) against the same enzyme and reaction buffer without lyophilization (Wet Reference) to demonstrate stable after lyophilization allowing for extended storage periods and use in diagnostic tests and POC devices.
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