Next-Generation Sequencing (NGS)

High sensitivity and yield PCR amplification of a 1.2 kb fragment of the F8 gene, from a five-fold serial dilution of human genomic DNA. All reactions were set up according to the manufacturers’ recommendations. Marker is HyperLadder ™ 50 bp, (BIO-33054) . Meridian’s High-Specificity Pfu HS Mix has higher sensitivity and yield over suppliers.

High-Specificity Pfu HS Mix

Pfu Turbo

Platinum SuperFi

Phusion II

Efficient amplification of DNA containing a wide range of GC-content

M 29% 32% 44% 55% 62% 65%

The following regions from human genomic DNA were amplified: RBB8 161 bp (29% GC), RBB8 450 bp (32% GC), IL 10 417 bp (44% GC), Mpex 418 bp (55% GC), EGFR 525 bp (62% GC), EGFR 380 bp (65% GC). Marker is HyperLadder ™ 50 bp, (BIO-33054 ). Efficient amplification was possible across the different GC content tested demonstrating the suitability of High-Specificity Pfu HS Mix for applications requiring low GC-bias.

Suitable for multiplex reactions from inhibitor-rich samples

High-Specificity Pfu HS

Platinum SuperFi

Pfu Turbo Phusion II

Multiplexed PCR amplification of 6 targets (961 bp, 892 bp, 793 bp, 548 bp, 332 bp, 196 bp) directly amplified from 20% whole blood and run in duplicate. Marker is HyperLadder ™ 50 bp, (BIO-33054) . High-specificity Pfu HS Mix is able to outperform other suppliers in the presence of 20% whole EDTA blood in a multiplexed PCR reaction.

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