STEP 3 PHOSPHORYLATION
Glycerol-Free T4 Polynucleotide Kinase (HC)
Cat #MDX206 VOLUME REACTIONS 25 μ L 500 Rxns 250 μ L 5,000 Rxns
Glycerol-Free T4 Polynucleotide Kinase (HC) (MDX206) is supplied at 20 U/ μ L and is used to phosphorylate the 5’ end of both DNA and RNA for subsequent ligation by catalyzing the transfer of the γ -phosphate from ATP to the 5´ hydroxyl of single stranded and double stranded DNA. Glycerol-Free T4 Polynucleotide Kinase (HC) is used in NGS library preparation, either separately or as part of an End-Repair Mix, where phosphates are added to the 5’-hydroxyl terminus of double stranded DNA for subsequent ligation of adaptors by T4 DNA Ligase.
Product Highlights • 5’ phosphorylation for
subsequent ligation for NGS. • Removes 3’ phosphoryl groups from DNA and RNA. • Concentration 20 U/ µ L
Fast kinetics with high performance
A
B
0.012
0.01
0.008
0.006
0.004
0.002
0
NEB
Meridian
NEB | MDX206
(Above) Increase in fluorescence over time and B/ phosphorylation reaction rate of Glycerol-Free T4 Polynucleotide Kinase (HC) ( blue ) and NEB’s T4 Polynucleotide Kinase ( red ) at a concentration of 0.005 U/ μ L. The results demonstrate that the glycerol-free enzymatic activity of T4 Polynucleotide Kinase produced by Meridian is comparable to glycerol-containing enzymes from other suppliers.
No loss of activity after lyophilization
(Left) Accelerated stability studies of Glycerol-Free T4 Polynucleotide Kinase at 37°C for two months in lyophilized ( red ) compared to the liquid ( blue ) format, stored at -20°C. The results demonstrate that Glycerol-Free T4 Polynucleotide Kinase is not affected by lyophilization and subsequent storage at ambient temperature fully retaining its activity when rehydrated.
Liquid | Lyophilized
4
Powered by FlippingBook