Library clean-up & size selection
Efficient removal of primers and adapters
Paramagnetic SPRI beads designed for clean-up and size selection of DNA fragments or NGS libraries. Paramagnetic beads selectively bind DNA fragments based on the volume ratio of bead suspension and sample. They are capable of delivering highly purified DNA fragments, efficiently removing contaminants, such as nucleotides, primers, adapters, enzymes, buffer additives and salts.
Electropherogram showing the library size profile after removal of unincorporated adapters and PCR primers (arrows) after using NGS Clean and Selection Beads.
PRODUCT
CAT NO.
VOLUME
50 mL
NGS Clean and Select Beads
MDX041
500 mL
Product Highlights • Highly-reproducible results • Tuneable size selection protocols for single or double sided selection • Compatible with manual and automated workflows
Size distribution after double-sided size selection
For double-sided size selection, supernatant from a right-side selection step (which excludes the largest library fragments) is re-purified using a different ratio of beads, according to the left-sided size selection protocol to exclude the smallest library fragments.
qPCR-based Quantification of NGS Libraries
Library Quantification
qPCR-based assay for the exclusive quantification of adapter-ligated molecules providing accurate quantification of Illumina NGS libraries. Contains pre-diluted standards to minimize pipetting errors, a P5 / P7 Illumina ® specific primer mix and an optimized buffer for dilution of NGS library samples.
PRODUCT
CAT NO.
REACTIONS
Library Quantification Kit
MDX039
500 Rxns / Kit
Product Highlights • Suitable for quantification of all Illumina compatible libraries • Accurate results in less than 90 minutes • Contains a series of six pre-diluted DNA standards for rapid and reliable standard curve generation, providing precise and accurate quantification • 18 sets of standards can be used for 62 libraries on six 96-well plates or 76 libraries on two 384-well plates
Standard curve generated from the amplifications of the 6 pre-diluted DNA standards (blue, 10 pM to 100 aM) and 3 dilutions of an Illumina ® NGS library (red, ten-fold series).
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
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08/23
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