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Figure 1. Distribution of detected pathogens by ddPCR and blood culture . Pathogens detected by ddPCR and blood culture within the range of ddPCR targeted organisms. Reproducible performance across both PCR mixes. Figure 2. The value of ddPCR in the dynamic surveillance of sepsis . The quantitative results of ddPCR align with the trends seen in CRP and PCT levels.
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Comparative Performance of Meridian's Chemistry Versus Commercial Alternatives on ddPCR
STUDY 2
Parallel tests were conducted using the same samples and reaction conditions with MDX024 and a mix from a second supplier, to assess amplification efficiency, specificity, inhibitor tolerance and stability after lyophilized.
STUDY RESULTS Under identical reaction conditions and thermal cycling protocols, templates of the same concentration were tested to compare the number of positive droplets and their fluorescence intensity distribution. MDX024 demonstrated higher amplification efficiency and improved uniformity [ Figure 3A ]. Negative samples were evaluated based on fluorescence intensity distribution to assess non- specific amplification. The MDX024 enzyme exhibited superior specificity, with no false positives arising from non-specific amplification [ Figure 3B ].
To evaluate inhibitor tolerance, 20 mg/mL of hemoglobin (a known PCR inhibitor in blood samples) was added to the reaction system. Under identical conditions, MDX024 generated more positive droplets than Supplier A, indicating greater resistance to inhibition [ Figure 3C ]. The enzyme's performance was also assessed in both liquid and lyophilized formats. MDX024 maintained its amplification performance across diverse templates before and after lyophilization, demonstrating strong compatibility with dry-down processes [ Figure 4 ].
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