Case Study: Rapid Sepsis Detection - ddPCR

A ddPCR AMPLIFICATION EFFICIENCY 3

SUPPLIER A

MDX024

B ddPCR AMPLIFICATION SPECIFICITY

C

INHIBITOR TOLERANCE IN ddPCR

SUPPLIER A

MDX024

SUPPLIER A

MDX024

Figure 3. Comparative results using Meridian MDX024 and a reference mix from Supplier A in the same reaction settings . (A) ddPCR amplification efficiency; (B) ddPCR amplification specificity; (C) Inhibitor tolerance to samples.

4

LYOPHILIZATION COMPATIBILITY

SUPPLIER A

MDX024

Liquid Lyophilized

Figure 4. Lyophilization compatibility. Comparative results using Meridian MDX024 and a reference mix from Supplier A in the same reaction settings.

Meridian’s Lyo-Ready 1-Step RT-qPCR Mix (MDX024) demonstrated strong amplification performance, high specificity, and compatibility with inhibitor-challenged samples and lyophilized formats in this ddPCR-based sepsis case study. These results support its suitability as a flexible reagent for ddPCR assay development in demanding molecular testing workflows.

meridianbioscience.com/lifescience

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