Respiratory Diseases

Antigen & Antibody Detection Assays SARS-CoV-2 | Continued Reagents for serology testing

9550

MAb to SARS-CoV-2 Trimeric Spike (S1) • Recognizes UK variant B.1.1.7 and South Africa variant B.1.351 • Immunogen: Recombinant SARS-CoV-2 S1 Protein • Binds to recombinant SARS-CoV-2 trimeric spike protein and can recognize the membrane bound form from the cell line expressing full-length SARS-CoV-2 protein (binds to a linear epitope so it is not confirmation dependent) • Does not cross-react with recombinant SARS-CoV, HCoV-229E, HCoV- HKU1, HCoVNL63 and HCoV-OC43 • Designed to work on saliva samples (no lysis required) • Capture antibody, can detect SARS-CoV-2 trimeric spike protein down to ~300 pg/mL by using TMB substrate in ELISA assay MAb to SARS-CoV-2 Trimeric Spike (S1) • Detection antibody MAb to SARS-CoV-2 Trimeric Spike (S1) • Additional detection antibody, 9551 and 9565 can be used together in a 1:1 ratio to increase assay sensitivity in ELISA

Paired MAbs for Sandwich ELISA & LF Antigen Detection Assays

9551

9565

SYNERGISTIC EFFECT OF CAT# 9551 & 9565 ENHANCES DETECTION OF SARS-CoV-2 TRIMERIC SPIKE PROTEIN

MAb sandwich ELISAs were compared for their ability to detect recombinant SARS-CoV-2 across 4 serial dilutions (10 ng/mL – 0.3 ng/mL). One assay used Cat# 9550 as the capture and Cat# 9551 as the detection antibody a second assay used Cat# 9550 as the capture and Cat# 9565 as the detection antibody and a third assays used Cat#9550 as the capture and Cat#9551 and Cat#9565 together in a 1:1 ratio as the detection antibodies. Using Cat# 9551 and 9565 in combination creates a synergistic effect that results in an enhanced detection of SARS-CoV-2 Trimeric Spike protein across all concentrations.

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Respiratory Diseases - Reagents for Assay Development

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