Respiratory Diseases

9566

SARS-CoV-2 Trimeric Spike (S1) Protein, Recombinant Antigen • Represents amino acids 14-683 of the full-length Spike Protein • Molecular Weight: ~ 480 kDa (on 4-16% Native SDS-PAGE) • Produced in insect cells, contains a C-terminal His-tag • Protein concentration 1.1 mg/mL (BCA), purity ≥ 85% (SDS-PAGE) • Buffer: PBS, pH 7.4 SARS-CoV-2 Trimeric Spike (S1) Protein, Recombinant Antigen • Molecular Weight: ~ 85 kDa (12% reducing SDS-PAGE) • Produced in insect cells, contains a C-terminal His-tag • Protein concentration 4.6 mg/mL (BCA), purity ≥ 85% (SDS-PAGE) • Buffer: PBS, pH 7.4 SARS-CoV-2 Spike (S1) N-Terminal Domain (NTD) Protein, Recombinant Antigen • Molecular Weight: ~ 40 kDa (12% reducing SDS-PAGE) • Produced in insect cells, contains a C-terminal His-tag • Protein concentration 1.8 mg/mL (BCA), purity ≥ 85% (SDS-PAGE) • Buffer: PBS, pH 7.4 SARS-CoV-2 Spike (S1) Receptor Binding Domain (RBD) Protein, Recombinant Antigen • Molecular Weight: two bands at ~ 28 and ~ 25 kDa (12% reducing SDS-PAGE) • Produced in insect cells, contains a C-terminal His-tag • Protein concentration 3.7 mg/mL (BCA), purity ≥ 85% (SDS-PAGE) • Buffer: PBS, pH 7.4 SARS-CoV-2 Trimeric Spike S1 (RBD) Protein, Recombinant Antigen • Molecular Weight: ~ 29.5 kDa • Produced in HEK293 cells, contains a C-terminal His-tag • Protein concentration 2.0 mg/mL (BCA), purity ≥ 90% (SDS-PAGE) • Buffer: PBS, pH 7.2 SARS-CoV-2 Spike (S2) Protein, Recombinant Antigen • Produced in insect cells, contains a C-terminal His-tag • Protein concentration 1.9 mg/mL (BCA), purity ≥ 85% (SDS-PAGE) • Buffer: PBS, pH 7.4 Human Angiotensin Converting Enzyme 2 (ACE2), Recombinant Antigen • Molecular Weight: ~ 110 kDa • Produced in HEK293 cells, contains a c-terminal human IgG1 Fc tag • Protein concentration 1.5 mg/mL (BCA Microplate Assay), purity ≥ 90% (SDS-PAGE) • Buffer: PBS, pH 7.2-7.4 SARS-CoV-2 Spike Protein, S1 Subunit, RBD, B.1.167.2 (Delta) Strain • Molecular weight: ~26kDa • Produced in insect cells, affinity purified (His-tag) ≥ 90% (SDS-PAGE) • Delta variant (L452R, T478K Mutations) • Concentration: Lot specific purity, mg/mL (BCA) • Buffer: Phosphate buffer solution, pH 7.4

9556

9557

9558

IgG and IgM Antibody Detection Assays

9559

9552

9555

9606

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