Product list | Continued
Troponin I-Skeletal (sTnI) The Troponin I subunit sTnI exists in three separate isoforms, two in skeletal muscle fibers and one in cardiac muscle (cTnI). Diagnosis of acute myocardial infarction by Troponin I is dependent on the detection of cTnI only (i.e. the assay should not be cross-reactive to Troponin I-skeletal). Antibodies made against this cardiac isoform are immunologically different from antibodies made against the two skeletal isoforms. Antibody Pairs • Cross-reactivity with Cardiac Troponin I is < 1.5% • Suitable for use in ELISA, WB and for immunopurification • MAbs produced in vivo CAPTURE DETECTION H86121M H86702M Antigens A86824H • Native antigen from human skeletal muscle • >95% pure (SDS-PAGE), lyophilized TroponinT-Cardiac (cTnT) TnT is a tropomyosin-binding subunit which regulates the interaction of Troponin Complex with thin myo-filaments. Two isoforms of TnT are expressed in human skeletal muscle tissue (sTnI and sTnT) and several isoforms are expressed in cardiac specific tissue (cTnT). Although assays for Cardiac Troponin T (cTnT) and Cardiac Troponin I (cTnI) exhibit similar clinical performance in patients with acute coronary syndromes for diagnosis and risk stratification, there are differences in the release and clearance of these proteins from damaged myocytes. Also cTNT has a higher overall tissue concentration and free cytoplasmic concentrations than cTnI, and appears in blood of patients with acute myocardial infarction as a mixture of complexed (cTnT-I-C) and free cTnT. Antibody Pairs • No cross-reactivity with human skeletal Troponin T • Suitable for use in ELISA and WB • MAbs produced in vivo CAPTURE DETECTION
Troponin I-Cardiac (cTnI) continued
H86207B H01246M H86286M H86241M H86596M
a.a. 41-49 a.a. 65-74
* Biotin conjugated
* Also works in IHC, reacts with free cTnI only (not complexed)
a.a. 86-90 a.a. 86-90
* Also works in IHC
a.a. 169-178 * Also works in IHC
Antibody Pairs • Suitable for use in ELISA • MAbs produced in vivo (unless otherwise noted) CAPTURE DETECTION H86465M H86458M
* Also works in WB and IP, no cross-reactivity with skeletal muscle troponin I
K31015M K31341G
* K31341G is a polyclonal (goat) cTnI, also works in IHC * K31342G is a polyclonal (goat) cTnI, also works in IHC
K31015M K31342G
H01326M H01325M H86285M H01347M H01395M H01343M H01395M H86465M H01395M H86280M H86625M H01395M H01395M H01396M H01340M H01396M Polyclonals CATALOG
* Also works in LF * Also works in WB
* Also works in WB * Also works in WB * Also works in WB
SOURCE FORMAT APPLICATION
K01350G Goat K31342G Goat
Aff.Pur. Aff.Pur.
EIA
EIA,IHC
* K31342G reacts with region a.a. 69-86 Antigens
BN1140
• Troponin C1 recombinant, N-terminal His-tag (E. coli) • >70% (SDS-PAGE) • Troponin C1 recombinant, GST tag (E. coli) • >70% (SDS-PAGE) • Troponin I3 recombinant, GST tag (E. coli) • >70% (SDS-PAGE) • Recombinant (E. coli) • 95% pure (SDS-PAGE Coomassie staining) • Suitable for use in ELISA • Native antigen from human heart • >98% pure (SDS-PAGE) • Suitable for use in ELISA, as antigen for antisera production, and as a tracer for iodination
BN1141
BN1142
H86111M H01363M H86111M H86707M H86111M H86906M H86429M H86906M H86429M H86111M H86429M H86519M
R01620
A86853H
A38150H
• Native antigen from human heart • >98% pure (SDS-PAGE)
20
Cardiac & Metabolic Markers for Assay Development
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