High Sensitivity and Reproducibility in Assays Detecting High or Low copies of DNA Templates in 10% Urine
The activity of Lyo-Ready ™ Genotyping Direct qPCR Urine was tested in a qPCR assay using a 10-fold serial dilution of synthetic DNA (10,000, 1000, 100, 10 and 1 copies respectively), in the presence of 10% pooled human urine. The results illustrate that Lyo-Ready ™ Genotyping Direct qPCR Urine has high sensitivity and reproducibility in assays detecting high or low copies of DNA templates and in the presence of PCR inhibitors.
1,000 copies 100 copies 10,000 copies
10 copies 1 copy
Stable Shelf Life for up to 18 months After Lyophilization
A)
Homozygous C/C Amplification Plot
Heterozygous C/A Amplification Plot
Homozygous A/A Amplification Plot
Allele A | Allele C
B)
Allelic Discrimination
Lyo-Ready ™ Genotyping Direct qPCR Urine was lyophilized, and the stability of the dried assays ( red ) was tested after 1 month at 37°C against fresh liquid mix ( blue ) in the presence of 10% pooled human urine. The lyophilized mixes retained A) the same performance as the liquid mix and B) the same accurate allelic discrimination (homozygous allele A ( red ) and allele C ( blue ) and heterozygous allele A/C ( green ) with a NTC ( black ) and x for undetermined). Results suggest that Lyo-Ready ™ Genotyping Direct qPCR Urine mix has a projected stability of more than 18 months at ambient temperature.
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