Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
A) Lyo-Ready™ DNA LAMP Blood (MDX124)
B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
HBV
HSV2
EBV
T. vaginalis
M. pneumoniae
HCV
HIV-1
DENGUE 1
WNV
Zika
Lyo-Ready ™ Direct DNA LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™
(A) Lyo-Ready ™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr virus (EBV), Mycoplasma pneumoniae and (B) Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue type 1, West Nile Virus (WNV) or Zika ( red ). The performance of the mixes were compared against NEB WarmStart ® LAMP kit ( orange ) and Thermo SuperScript ™ IV RT-LAMP Master Mix ( black) using the same primers and DNA/RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready ™ Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA and viral RNA targets.
High Assay Sensitivity and Reproducibility in Both Liquid and Lyophilized Formats
(A) Lyo-Ready™ DNA LAMP Blood (MDX124)
(B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
1000
100
10
1
NTC
1000
500
200
50
NTC
Lyophilized-Ready ™ Direct LAMP Blood | Liquid Direct LAMP Blood | NEB WarmStart ® | Thermo SuperScript ™
(A) Lyo-Ready ™ Direct DNA LAMP Blood (MDX124) ( red ) was lyophilized with T. vaginalis primers and used for the amplification of 1,000, 100, 10 and 1 copy per reaction of the bacterial genomic DNA and (B) Lyo-Ready ™ Direct RNA/DNA LAMP Blood (MDX125) ( red ) was lyophilized with HIV-1 primers and used for the amplification of 1,000, 500, 200 and 50 copies per reaction of the viral RNA. The performance of the mixes were compared against liquid formats of Lyo-Ready ™ Direct LAMP Blood mixes (MDX124 or MDX125, blue ), NEB WamStart ® LAMP kit (DNA & RNA) ( orange ) and Thermo SuperScript ™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were incubated at 65 °C for 60 minutes. Error bars represent standard deviation across three technical replicates. The results illustrate the increased sensitivity and specificity of the Lyo-Ready ™ Direct LAMP Blood mixes compared to other mixes, with earlier TTR and delayed NTC.
Powered by FlippingBook