Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety.
Glycerol-Free Tth DNA Polymerase (HC) High concentration thermostable
DNA polymerase with intrinsic reverse transcriptase activity.
Meridian’s new Glycerol-Free HS Tth DNA Polymerase (HC) is a hot-start thermostable DNA polymerase with intrinsic reverse transcriptase activity supplied in a high concentration (200 U/ µ L) format. Tth polymerase is derived from the thermophilic bacteria Thermus thermophilus (Tth) and has a 5‘- 3’ exonuclease activity and lacks both 3’-5’ exonuclease and RNase H activities. It can perform both reverse transcription and DNA amplification at elevated temperatures (+55°C to +70°C), enabling amplification directly from RNA and better amplification sensitivity for templates with high secondary structures, such as RNA viruses.
Glycerol-Free HS Tth DNA Polymerase uses antibody-mediated hot start technology to further enhance assay specificity and sensitivity and is supplied with a 5x Reaction Buffer that contains dNTPs and excipients essential for lyophilization. The master mix can be used to develop point-of-care friendly, ambient-temperature stable assays, or high-throughput automated machines requiring low-viscosity formulations to facilitate robotic pipetting.
• Amplify DNA or RNA in a single step at elevated temperatures (+55°C to +70°C) • Increase assay sensitivity for targets with complex secondary structures • Supplied in a high 200 U/ µ L concentration format, glycerol-free, and with excipients essential for lyophilization • Ideal for point-of-care ambient temperature stable assays or high-throughput automated machines requiring low-viscosity formulations to facilitate robotic pipetting
Maintains Thermostability at 95°C for 10 Minutes
Glycerol-Free HS Tth DNA Polymerase (HC)
Performance of Glycerol-Free HS Tth DNA Polymerase (MDX205, red ), an MMLV-RT ( blue ) and an AMV-RT ( black ) after pre-incubation from 40°C to 95°C for 10 mins, in a multiplex one-step RT-qPCR assay on mouse RNA. ∆ Ct values were calculated against the Ct value produced by the same enzyme stored at -20 °C. The data illustrates that MDX205 maintains its full performance even after 10 min at 95°C, in contrast to MMLV-RT and AMV-RT which are inactivated above 55°C and 60°C, respectively.
REACTIONS 4,000 Rxns 100,000 Rxns
Superior Performance and Sensitivity Compared to HawkZ05 DNA Polymerase
A) DNA Template
B) RNA Template
MDX205 | Roche
Glycerol-Free HS Tth DNA Polymerase (MDX205, red ) was lyophilized in Lyo-Ready ™ Tth Reaction Buffer and subsequently tested against HawkZ05 Fast Polymerase (Roche, green ) in A) a qPCR or B) an RT-qPCR reaction in the presence of mammalian DNA or RNA template. Results demonstrate that Glycerol-Free HS Tth DNA Polymerase has better performance and sensitivity than enzymes from other suppliers.
Maintains a Stable Shelf Life for up to 12 months after Lyophilization
A) DNA Template
B) RNA Template
Lyophilized | Liquid mix
Glycerol-Free HS Tth DNA Polymerase (MDX205) was lyophilized ( red ) in Lyo-Ready ™ Tth Reaction Buffer and subsequently stored for 1 month at 37°C for accelerated stability studies. The enzyme was tested in triplex reactions with A) mammalian DNA or B) mammalian RNA against fresh liquid MDX205 ( blue ) stored at -20°C. Results suggest that the lyophilized mix is active following accelerated stability tests with projected 12 months stability at ambient temperature.
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