ToRCH & Childhood Diseases

The most suitable proteins are reportedly: • C MV pp150: a tegument phosphoprotein detectable during both latent and re-activated infections. During primary infection the antibody response to pp150 may be delayed. • C MV pp52: the major DNA binding protein, nonstructural nuclear phosphoprotein which is regarded as an early marker of seroconversion • C MV pp65: major structural phosphoprotein (lower matrix) and main component of extracellular virus particles. The antibody response is detectable during early infection only • C MV gB Antigen: a membrane glycoprotein which is the most abundant component of the viral envelope and a target of neutralizing antibodies • C MV pp38: structural protein suggested to be an important immunodominant protein in early infection Evidence also suggests that CMV-IgM detection against viral structural proteins (pp150 and pp38) are a valuable parameter for the early diagnosis of a recurrent CMV infection. Several diagnostic manufacturers have incorporated a combination of CMV lysate and CMV recombinant proteins to improve assay performance. REAGENTS FOR SEROLOGY TESTING CMV-G Native Antigen • Whole cell extract, >10% viral protein • Strain AD169 propagated in human fibroblast cells 7504

• 0.25 mg/mL by OD 260/280nm • Buffer: 0.1M Glycine, pH 9.3-9.7

7517

CMV Ext-2 Native Antigen (Concentrate) • Enriched for cell surface glycoprotein antigens, >10% viral protein • Strain AD169 propagated in human fibroblast cells • Protein concentration: 0.2-1mg mg/mL by OD 260/280nm • Buffer: 0.1M Glycine, pH 9.5±0.2 CMV Native Antigen • Viral lysate prepared by centrifugation to remove cell debris

7600

• Strain AD169 propagated in MRC-5 cells • Buffer: Glycine buffered saline, pH 9.5 CMV gB Native Antigen • Strain AD169 propagated in human fibroblast cells • Buffer: 0.1M Glycine, pH 9.5 ± 0.2 5

IgG Detection for EIA Assays

EV7509

R18102

CMV gB Recombinant Antigen • Contains the CMV gB immunodominant region (Strain C194) and a GST fusion partner • Immunoreactive with CMV positive sera • Produced in E. coli , >95% pure (SDS-PAGE) • Buffer: 25mM Tris-HCl, 1mM EDTA, pH 7.2 and 50% glycerol

R01686

CMV IgM Native Antigen (Concentrate) • Preparation of nuclear extract and ER antigens • Strain AD169 propagated in human fibroblast cells • Buffer: 0.1M Glycine, pH 9.3-9.7 CMV Chimeric Recombinant Antigen • Produced in E. coli , >95% pure (SDS-PAGE) • 31.6kDa calculated MW • Buffer: 20 mM NaP @ pH8, 1 M NaCl

7511

IgM Detection for EIA Assays