ToRCH & Childhood Diseases

CMV

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REAGENTS FOR SEROLOGY TESTING (cont)

EV9268

CMV II Native Antigen • Purified surface extract containing pp65, pp52, pp150 and other key proteins • Strain AD169 propagated in human fibroblast cells • Buffer: 0.1M Glycine, pH 9.5±0.2

7507

CMV III Native Antigen • Purified surface extract enriched for pp65, >60% viral protein • Strain AD169 propagated in human fibroblast cells • Protein concentration: 0.8-1.2mg/mL by BCA • Buffer: 0.1M Glycine, pH 9.3-9.7 CMV Native Antigen • Viral lysate prepared by centrifugation to remove cell debris

IgG & IgM Detection for EIA Assays

7600

• Strain AD169 propagated in MRC-5 cells • Buffer: Glycine buffered saline, pH 9.5

Suitable for use as a Control or for Antibody Detection in Lateral Flow Assays

R01763

Coxsackievirus A16 Recombinant Antigen • Enterovirus A • Produced in E. coli, contains a GST fusion partner • Buffer: Tris buffer with salts, pH 8.0, 0.09% Sodium Azide

CMV pp52 (UL44) Recombinant Antigens

R01565

• Represents a truncated portion of CMV pp52 gene fused to a GST-tag • Molecular weight of 44kDa • Produced in E. coli , ≥ 95% pure (PAGE) • Buffer: 50mM Tris-HCl, pH 7.2 containing 60mM NaCl, 10mM GSH, 50% Glycerol • Represents a truncated portion of CMV pp52 gene fused to a His-tag at the N-terminus • Produced in E. coli , ≥ 95% pure (SDS-PAGE) • Buffer: 20mM Sodium Phosphate, 1M NaCl, pH 8.0 • Represents residues 202-434 of CMV pp52, fused with a GST tag at the N-terminus • Molecular weight of 51kDa • Produced in E. coli , ≥ 90% pure (SDS-PAGE) • Buffer: 50mM Tris-HCl, 60mM NaCl, 10mM GSH, 0.25% Sarcosyl, 50% Glycerol, pH 8.0 • Represents CMV pp65 (strain AD169) gene fused to a GST-tag • Molecular weight of 50kDa • Produced in E. coli , ≥ 95% pure (SDS-PAGE) • Buffer: 25mM Tris-HCl, 1mM EDTA, pH 7.2 containing 50% Glycerol • Represents CMV pp65 gene fused to a His-tag at the N-terminus • Molecular weight of 35kDa • Produced in E. coli , ≥ 95% pure (SDS-PAGE) • Buffer: 20mM Sodium Phosphate, 1M NaCl, 0.1% Polyoxyethylene (10) Tridecyl Ether and 8M Urea, pH 8.0

R01561

R18062

IgM Detection for EIA Assays

R01562

R18412

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ToRCH & Childhood Diseases- Reagents for Assay Development

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