Superior Assay Sensitivity and Reproducibility with Lower TTR and Delayed NTC
A) Lyo-Ready™ Direct DNA LAMP Urine (MDX154)
B) Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)
Sample NTC N.gonorrhoeae
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample NTC CHIKV
Sample NTC DENV
Sample NTC HCV
Sample NTC WNV
Sample NTC WNV
Sample NTC YFV
HSV1
L.interrogans
M.genitalium
T.vaginalis
Lyophilized Mix | NEB WarmStart® | Thermo SuperScript™
(A) Lyo-Ready™ Direct DNA LAMP Urine (MDX154, red ) was lyophilized with primers for Neisseria gonorrhoeae, Leptospira interrogans, Mycoplasma genitalium, Trichomonas vaginalis and Herpes Simplex Virus 1 (HSV1) and (B) Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155, red ) was lyophilized with primers for Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), West Nile Virus (WNV), Zika Virus (ZIKV) or Yellow Fever Virus (YFV). The performance of the mixes was compared against NEB WarmStart ® LAMP (DNA & RNA) ( orange ) and Thermo SuperScript™ IV RT-LAMP ( black ) using the same primers and corresponding DNA/RNA templates. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready™ Direct LAMP Urine mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across a range of pathogenic DNA and viral RNA targets.
Ultra-Sensitive Detection Down to 1 Copy of DNA or 50 Copies of RNA
(A) Lyo-Ready™ DNA LAMP Urine (MDX154)
(B) Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)
10 15 20 25 30 35 40
0 5
2000
500
100
50
NTC
Copies/rxn Copies/rxn
Copies/rxn
Lyophilized | Wet | NEB WarmStart® | Thermo SuperScript™
(A) Lyo-Ready™ Direct DNA LAMP Urine (MDX154) ( red ) was lyophilized with T. vaginalis primers and used for the amplification of 1,000, 100, 10 and 1 copies per reaction of the genomic DNA in the presence of 20% human urine and (B) Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155) ( red ) was lyophilized with Zika primers and used for the amplification of 2,000, 500, 100 and 50 copies per reaction of the viral RNA in the presence of 5% human urine. The performance of the mixes was compared against liquid formats of Lyo-Ready™ Direct LAMP Urine (MDX154 or MDX155, blue ), NEB WamStart ® LAMP kit (DNA & RNA) ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were incubated at 65 °C for 60 minutes. Error bars represent standard deviation across three technical replicates. The results illustrate the increased sensitivity and specificity of the Lyo- Ready™ Direct LAMP Urine mixes compared to other mixes, with earlier TTR and delayed NTC.
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