Results Delivered in Half the Time with Samples Containing 20-30% Urine
(A) Lyo-Ready™ DNA LAMP Urine (MDX154)
HSV1 + 30% Urine
60
50
40
30
N. gonorrhoea + 30% Urine
20
10
0
HSV1 +20% Urine
N.gonorrhoeae +20% Urine
L. interoggans +30% Urine
M. genitalium +20% Urine
C. trachomatis +30% Urine
T. vaginalis +30% Urine
Dengue Type-1 + 20% Urine
(B) Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)
Chikungunya + 5% Urine
CHIKV + 5% Urine
HCV + 5% Urine
ZIKV + 20% Urine
WNV + 5% Urine
DENV + 20% Urine
Lyophilized | NEB WarmStart® | SuperScript™
(A) Lyo-Ready™ Direct DNA LAMP Urine (MDX154, red ) was lyophilized with primers for Neisseria gonorrhoeae, Leptospira interrogans, Mycoplasma genitalium, Chlamydia trachomatis, Trichomonas vaginalis and Herpes Simplex Virus 1 (HSV1) and (B) Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155, red ) was lyophilized with primers for either Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), Zika, West Nile Virus (WNV) or Dengue Type-1. The performance of the mixes was compared against liquid formats of NEB WarmStart ® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that both MDX154 and MDX155 have earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready™ Direct DNA LAMP Urine (MDX154) and Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155) perform better and with faster amplification across the range of inhibitors compared to the other
mixes.
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