Stable Self-Life For Over 12 Months After Lyophilization (A) Lyo-Ready™ DNA LAMP Urine (MDX154)
(B) Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)
Wet | Lyophilized
(A) Freshly prepared Lyo-Ready™ Direct DNA LAMP Urine (MDX154, blue ) and (B) Lyo-Ready™ Direct RNA/DNA LAMP Urine mix (MDX155, blue ) were compared to lyophilized mixes ( red ) that had been incubated for 1 month at 37°C in an accelerated stability study. MDX154 was used to amplify T. vaginalis in the presence of 20% human urine and MDX155 was used to amplify Zika virus RNA in the presence of 5% human urine. Four technical replicate reactions were incubated at 65 °C for 60 minutes. The amplification plot between fresh liquid mix and lyophilized mix stored for 1 month at 37°C are overlaying, suggesting higher stability and reproducibility of the mixes, with a projected stability of greater than 12 months at ambient temperature.
References: 1. Bent S., et al. Does this woman have an acute uncomplicated urinary tract infection? JAMA (2002);287:2701 2. Giesen L.G.M., et al. Predicting acute uncomplicated urinary tract infection in women: a systematic review of the diagnostic accuracy of symptoms and signs. BMC Fam Pract (2010);11:78. 3. Baker C. Accident and emergency statistics: demand, performance and pressure. House Commons Libr (2017);6964:1–32.
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