Meridian Non-Bac DNA qPCR Solutions

Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection

MDX362 Performance Data

Designed for Sensitive Bacterial DNA Detection The completed 4x hot-start mix containing optimized buffer chemistry, PCR enhancers, dNTPs (including dUTP), MgCl 2 and Glycerol-Free Non-Bac HS Taq polymerase, supports low-copy and multiplex qPCR testing. It can be used as a liquid mix or lyophilized for ambient-stable assay formats.

10,000 copies 1,000 copies 100 copies 10 copies 1 copy

Lyo-Ready Non-Bac DNA qPCR Mix in liquid (blue) and lyophilized (red) format s were compared for their ability to amplify a 10-fold serial dilution of a bacterial DNA target (10,000, 1,000, 100, 10 and 1 copies respectively). The results illustrate that the lyophilized mix maintains the same high application efficiency as the liquid mix, with high sensitivity and reproducibility. NTCs are shown in (black) .

No Detectable Bacterial DNA Contamination

Using a pan-bacterial 16S assay, MDX362 showed high sensitivity and reproducibility while the no-template control remained without detectable amplification. Lyo-Ready Non-Bac DNA qPCR Mix was used to amplify a 10-fold serial dilution of bacterial DNA target (10,000, 1,000, 100, 10, and 1 copies, respectively) in liquid (blue) format using a pan-bacterial 16S assay. No detectable amplification was observed in the no-template control (black) . The Lyo-Ready Non-Bac DNA qPCR Mix demonstrates high

10,000 copies 1,000 copies 100 copies 10 copies 1 copy

sensitivity and reproducibility, supporting reliable detection of low-copy bacterial DNA targets with no detectable bacterial background amplification under the conditions tested.