Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX252 Performance Data
High Multiplexing Capability 16S (Entero)
Mip
ToxA
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
The activity of the Glycerol-Free Non-Bac HS Taq Polymerase (HC) was tested in a triplex qPCR assay targeting marker genes from three bacteria (16S rRNA gene from Enterococcus , Mip gene from Legionella pneumophila and ToxA gene from Pseudomonas aeruginosa ), using a 10-fold serial dilution of the bacterial genomic DNA. The result shows that Glycerol-Free Non-Bac HS Taq Polymerase (HC) can successfully detect multiple targets down to 1 copy of DNA in multiplex assays.
Lyophilized Assay Stability Glycerol-Free Non-Bac HS Taq Polymerase (HC) was lyophilized in a lyo-ready DNA qPCR mix and the stability of the dried assay (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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