qPCR and RT-qPCR Mixes
Universal
Universal Mixes
Meridian’s Lyo-Ready and Air-Dryable™ qPCR and RT-qPCR master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various qPCR inhibitors and can be used across a wide range of patient sample types.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 500 Rxns 100 mL 10,000 Rxns 8 mL 1,000 Rxns 100 mL 12,500 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns
Air-Dryable qPCR Mix, 4x
MDX082
Air-Dryable RT-qPCR Mix, 4x MDX095
Lyo-Ready qPCR Mix
MDX021
Lyo-Ready qPCR Mix, 2.6x
MDX023
Lyo-Ready 1-Step RT-qPCR Mix
MDX024
Lyo-Ready 1-Step RT-qPCR Virus Mix
MDX062
Full Enzyme Activity Following Lyophilization/Air-Drying
Air-Dryable™ RT-qPCR Mix (MDX095)
Activity of Air-Dryable™ 1-Step RT-qPCR Mix (MDX095) in both wet and air-dried formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mixup to the assay limit of detection.
Wet | Air-dried
Retention of Reverse Transcriptase Activity Following Lyophilization
Lyo-Ready 1-Step RT-qPCR Mix (MDX024)
y-actin
GAPDH
B2mg
Primers and probes were added to Lyo-Ready 1-Step RT-qPCR Mix (MDX024), dried down and immediately rehydrated ( purple ) and tested against a freshly prepared wet mix ( orange ), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.
Lyophilized | Wet Mix
Powered by FlippingBook