Lyo-Ready Direct Stool Mixes

Ideal for Liquid Format or Lyophilized Assays – No Impact on Reaction Efficiency

A) Lyo-Ready ™ Direct DNA qPCR Stool (MDX142)

B) Lyo-Ready ™ Direct RNA/DNA qPCR Stool (MDX143)

10,000 copies 1,000 copies

100 copies 10 copies

lyophilized | liquid

Liquid ( blue ) and lyophilized ( red ) formats of A) Lyo-Ready ™ Direct DNA qPCR Stool (MDX142) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100, and 10 copies respectively) in presence of 20% artificial stool and B) Lyo-Ready ™ Direct RNA/DNA qPCR Stool (MDX143) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively) in presence of 5% artificial stool. The results illustrate that the lyophilized mixes retain the ability to efficiently amplify to the same level as the liquid mixes with the same level of sensitivity and reproducibility.

High Performance with Both Standard and Fast Cycling Conditions Provides Greater Assay Flexibility and Reduced Run Times

Lyo-Ready™ Direct DNA qPCR Stool (MDX142)

Standard cycling

Fast cycling

Two mammalian DNA targets ( red ) were amplified using standard qPCR cycling conditions and the fastest cycling conditions available on a Quantstudio ™ 7 Flex Real-Time PCR System in the presence of 6.6 mg/mL human stool. The results illustrate the higher performance and end-fluorescence of Lyo-Ready ™ Direct DNA qPCR Stool (MDX142) in both standard (3 min @ 95ºC, 40 cycles of 10 sec @ 95ºC and 30 sec @ 60ºC) and fast (3 min @ 95ºC, 40 cycles of 1 sec @ 95ºC and 1 sec @ 60ºC) qPCR cycling conditions, without compromising the sensitivity and reproducibility of an assay.