Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Blood-Specific Mixes
PRODUCT
CAT. NO.
VOLUME REACTIONS
Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
5 mL
800
A MDX124 | Lyo-Ready Direct DNA LAMP Blood
B MDX125 | Lyo-Ready Direct RNA/DNA LAMP Blood
Lyo-Ready Direct DNA LAMP Blood Mix
MDX124
50 mL
8,000
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Blood Mix
MDX125
50 mL
8,000
Blood is one of the most common specimens used for laboratory diagnostic testing and is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer, cardiovascular disease, and metabolic disorders including diabetes. Bacterial cell culture is regarded as the gold standard for microbial detection from blood as molecular methods to-date have lacked the sensitivity to detect bacteria at low concentrations due to the presence of assay inhibitors among other factors. Meridian’s Lyo-Ready Direct DNA and RNA/DNA LAMP Blood Mixes are the newest solution for assays requiring high sensitivity and fast time to results and can detect DNA or RNA down to single copies in blood, serum or plasma samples. The mixes are designed to be extremely inhibitor-tolerant, enabling direct detection using crudely processed samples, making them ideal for developing next-generation, culture-free assays detecting bacterial infections such as sepsis. They outperform other commercial LAMP mixes in terms of sensitivity, specificity and speed which is demonstrated by their quicker time-to-result (TTR) and lower non-specific amplification (NTC) across a range of targets. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready Direct DNA LAMP or RNA/ DNA LAMP Blood mix, assay development time for a new ultra-sensitive direct blood assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
MDX124/MDX125 | NEB WarmStart ® | Thermo SuperScript TM
(A) Lyo-Ready Direct DNA LAMP Blood ( MDX124 - red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr Virus (EBV) or Mycoplasma pneumoniae , and (B) Lyo-Ready Direct RNA/DNA LAMP Blood ( MDX125 - red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue Type 1, West Nile Virus (WNV) or Zika. The performance of the mixes was compared against NEB WarmStart ® LAMP Kit (green) and Thermo SuperScript TM IV RT-LAMP Master Mix (blue) using the same primers and DNA/RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo-Ready Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across the range of pathogenic DNA and viral RNA targets.
Higher Specificity in the Presence of Whole Blood K2-EDTA or Plasma
A MDX124 | Lyo-Ready Direct DNA LAMP Blood
B MDX125 | Lyo-Ready Direct RNA/DNA LAMP Blood
MDX124/MDX125 | NEB WarmStart ® | Thermo SuperScript TM
(A) Lyo-Ready Direct DNA LAMP Blood ( MDX124 - red ) was lyophilized with primers for HBV, EBV, P. malaria , P. pneumoniae or HSV2, and (B) Lyo-Ready Direct RNA/DNA LAMP Blood ( MDX125 - red ) was lyophilized with primers for Dengue Type 1, HCV, West Nile Virus (WNV), Zika, or HIV-1. Their performance was tested against NEB WarmStart ® LAMP Kit (green) and Thermo SuperScript TM IV RT-LAMP Master Mix (blue). Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. The data illustrates better performance with faster amplification across the range of inhibitors for both Lyo-Ready Direct LAMP Blood mixes, with earlier TTRs across a broad range of pathogenic targets.
meridianbioscience.com/lifescience
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