Inhibitor-Tolerant LAMP & RT-LAMP Mixes
Saliva-Specific Mixes
High Inhibitor Tolerance and Fast Amplification to Deliver Quicker Time-to-Results (TTR) in Saliva, Sputum and Samples Containing Universal Transport Media (UTM)
PRODUCT
CAT. NO.
VOLUME REACTIONS
5 mL
800
Lyo-Ready Direct DNA LAMP Saliva Mix
MDX134
MDX134 | Lyo-Ready Direct DNA LAMP Saliva
50 mL
8,000
70 60 50 40
70 60 50 40 20 10 0 30
70 60 50 40 20 10 0 30
CMV
HSV2
ADV
5 mL
800
Lyo-Ready Direct RNA/DNA LAMP Saliva Mix
MDX135
50 mL
8,000
30
20 10 0
Saliva is ideal for use as a diagnostic specimen as it contains hormones, antibodies, growth factors, enzymes and microbes that can be used as biomarkers for disease detection. Saliva is easy-to-collect and allows non-invasive and simple sample collection/logistics, making it perfect for point of care testing. However, due to the low concentration of analytes and high concentration of inhibitors in saliva, its use in molecular diagnostics has been limited. Isothermal amplification such as LAMP is very tolerant to inhibitors and the simple workflow allows fast, sensitive, reliable results in clinical diagnosis. Meridian’s new Lyo-Ready Direct DNA and RNA/DNA LAMP Saliva Mixes are the newest advancement in molecular reagent technology and combine the benefits of a highly inhibitor-tolerant mix, with robust isothermal amplification, to enable direct detection using crudely processed samples. The mixes are uniquely designed to overcome saliva and sputum inhibitors and they do not require a DNA or RNA purification step to purify a patient sample. In addition, the 4x concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient-temperature stable assays. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready Direct DNA LAMP or RNA/ DNA LAMP Saliva mix, assay development time for a new ultra-sensitive direct saliva assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
NTC
NTC
NTC
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
0% Inhibitor
30% Saliva
30% UTM + Sputum
30% VTM
MDX134 (Lyophilized) | NEB WarmStart ®
Primers for CMV, ADV or HSV-2 were added to Lyo-Ready Direct DNA LAMP Saliva ( MDX134 - blue ) and lyophilized, or added to NEB WarmStart ® ( green ) and kept as a liquid. The mixes were amplified using inactivated Cytomegalovirus (CMV), Adenovirus (ADV) or Herpes Simplex Virus 2 (HSV2). Reactions were prepared in the presence of 30% human saliva, 30% Universal Transport Medium (UTM) with an artificial sputum swab, and Viral Transport Medium (VTM). All reactions were incubated at 65°C for 60 minutes, and average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. Results show that MDX134 (blue) has a shorter TTR than NEB WarmStart ® (green) amplification across all three sample types. The data suggests that MDX134 performs better with faster amplification across the range of inhibitors.
Superior Specificity Using Samples Containing 15% Sputum
MDX134 | Lyo-Ready Direct DNA LAMP Saliva
Amplification of Mycoplasma pneumoniae genomic DNA (200 copies) was compared using Lyo-Ready Direct DNA LAMP Saliva (MDX134) against NEB WarmStart ® in samples containing 0% or 15% artificial sputum. Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. No NTCs were detected in this assay. Lyo-Ready Direct DNA LAMP Saliva ( MDX134 ) with 0% ( light blue ) and 15% ( dark blue ) artificial sputum demonstrated stronger inhibitor tolerance, retaining its fast TTR in the presence of 15% artificial sputum. In contrast, NEB WarmStart ® with 0% artificial sputum ( orange ) amplified slower, as shown by a later TTR, than either reaction using Lyo-Ready Direct DNA LAMP Saliva. In addition, in samples containing 15% artificial sputum ( red ), NEB WarmStart ® had a delayed amplification of 40 min and the longest TTR of all the reactions.
MDX134 0% Sputum | MDX134 15% Sputum NEB WarmStart ® 0% Sputum | NEB WarmStart ® 15% Sputum
Maintains Consistent Performance in Samples Containing up to 60% Saliva MDX135 | Lyo-Ready Direct RNA/DNA LAMP Saliva
Lyo-Ready Direct RNA/DNA LAMP Saliva ( MDX135 ) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10% - 72%). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo-Ready Direct RNA/DNA LAMP Saliva maintains robust performance up to 60% saliva and demonstrates consistently late NTCs.
25
20
15
10
5
0
0%
10%
20%
30%
50%
60%
Saliva (%)
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