Lyo-Ready 1-Step RT-qPCR Mixes Lyophilization-compatible RT-qPCR master mixes that are ideal for high-throughout and point-of-care diagnostic assays.
Lyo-Ready 1-Step RT-qPCR master mixes are glycerol-free formulations that contain Taq polymerase, reaction buffer, dNTP, MgCl 2 , lyo-excipients and separate reverse transcriptase and dilution buffer. They are ideal for developing room temperature, lyophilized, multiplex RT-qPCR diagnostic tests and are suited for high-throughput, automated platforms requiring milli-second rehydration times.
Optimized 1-Step RT-qPCR Master Mixes for Lyophilization • Provided glycerol-free and pre-formulated with a specialized blend of lyo-excipients • Virus mix (Cat #MDX062) is specifically optimized for amplification of RNA or DNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C) • Suited for multiplex-assays and low-copy number targets • Compatible with lyophilization into beads or cakes • Ideal for developing fast, highly reproducible assays requiring ambient temperature transport and storage
Lyo-Ready ™ 1-Step RT-qPCR Mix MDX024
Lyo-Ready ™ 1-Step RT-qPCR Virus Mix
High efficiency and sensitivity from both DNA and RNA templates Amplification of both Rotavirus A (dsRNA) and Adenovirus (dsDNA) in a single multiplexed RT-qPCR assay, from inactivated crude viral lysates. The result illustrates that both viruses were amplified with high efficiency and sensitivity, demonstrating the ability of Lyo-Ready ™ 1-Step RT-qPCR Virus Mix (Cat #MDX062) to detect
Rotavirus A Adenovirus
low-copy number RNA and DNA targets simultaneously from a single sample.
Inhibitor tolerant enabling amplification directly from blood samples γ -actin was amplified from mouse RNA in the presence of 2% whole blood using Lyo-Ready ™ 1-Step RT-qPCR Mix (purple traces) and a competitor mix (green traces). The results demonstrate the ability of Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024) to amplify from difficult samples such as blood, in contrast to the competitor mix which fail, even with higher concentrations of the template RNA.
Lyo-Ready 1-Step Mix Competitor Mix
Retention of reverse transcriptase activity following lyophilization
Primers and probes were added to Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024), dried down and immediately rehydrated (purple) and tested against a freshly prepared wet mix (orange), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.
Lyophilised | Wet Mix
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