Lyo-Ready 1-Step RT-qPCR Mixes

Lyophilization-compatible RT-qPCR master mixes that are ideal for high-throughout and point-of-care diagnostic assays.

Lyo-Ready 1-Step RT-qPCR Mixes Lyophilization-compatible RT-qPCR master mixes that are ideal for high-throughout and point-of-care diagnostic assays.

Suitable Applications

Food Testing

Environmental

Human Diagnostics

Vet Health

Lyo-Ready 1-Step RT-qPCR master mixes are glycerol-free formulations that contain Taq polymerase, reaction buffer, dNTP, MgCl 2 , lyo-excipients and separate reverse transcriptase and dilution buffer. They are ideal for developing room temperature, lyophilized, multiplex RT-qPCR diagnostic tests and are suited for high-throughput, automated platforms requiring milli-second rehydration times.

Optimized 1-Step RT-qPCR Master Mixes for Lyophilization • Provided glycerol-free and pre-formulated with a specialized blend of lyo-excipients • Virus mix (Cat #MDX062) is specifically optimized for amplification of RNA or DNA viruses with a high secondary structure (reverse transcriptase remains active at 55-60 °C) • Suited for multiplex-assays and low-copy number targets • Compatible with lyophilization into beads or cakes • Ideal for developing fast, highly reproducible assays requiring ambient temperature transport and storage

PRODUCT

CAT NO.

VOLUME REACTIONS

10 mL

1,000 Rxn

Lyo-Ready ™ 1-Step RT-qPCR Mix MDX024

100 mL

10,000 Rxn

10 mL

1,000 Rxn

Lyo-Ready ™ 1-Step RT-qPCR Virus Mix

MDX062

100 mL

10,000 Rxn

www.MeridianLifeScience.com

Product Highlights

High efficiency and sensitivity from both DNA and RNA templates Amplification of both Rotavirus A (dsRNA) and Adenovirus (dsDNA) in a single multiplexed RT-qPCR assay, from inactivated crude viral lysates. The result illustrates that both viruses were amplified with high efficiency and sensitivity, demonstrating the ability of Lyo-Ready ™ 1-Step RT-qPCR Virus Mix (Cat #MDX062) to detect

Rotavirus A Adenovirus

low-copy number RNA and DNA targets simultaneously from a single sample.

Inhibitor tolerant enabling amplification directly from blood samples γ -actin was amplified from mouse RNA in the presence of 2% whole blood using Lyo-Ready ™ 1-Step RT-qPCR Mix (purple traces) and a competitor mix (green traces). The results demonstrate the ability of Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024) to amplify from difficult samples such as blood, in contrast to the competitor mix which fail, even with higher concentrations of the template RNA.

Lyo-Ready 1-Step Mix Competitor Mix

Retention of reverse transcriptase activity following lyophilization

Primers and probes were added to Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024), dried down and immediately rehydrated (purple) and tested against a freshly prepared wet mix (orange), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.

γ -actin

Gapdh

B2mg

Lyophilised | Wet Mix

Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Fax: +1 901-333-8223 Toll Free: +1 800 327 6299

Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.MeridianLifeScience.com

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