High efficiency and sensitivity from both DNA and RNA templates Amplification of both Rotavirus A (dsRNA) and Adenovirus (dsDNA) in a single multiplexed RT-qPCR assay, from inactivated crude viral lysates. The result illustrates that both viruses were amplified with high efficiency and sensitivity, demonstrating the ability of Lyo-Ready ™ 1-Step RT-qPCR Virus Mix (Cat #MDX062) to detect
Rotavirus A Adenovirus
low-copy number RNA and DNA targets simultaneously from a single sample.
Inhibitor tolerant enabling amplification directly from blood samples γ -actin was amplified from mouse RNA in the presence of 2% whole blood using Lyo-Ready ™ 1-Step RT-qPCR Mix (purple traces) and a competitor mix (green traces). The results demonstrate the ability of Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024) to amplify from difficult samples such as blood, in contrast to the competitor mix which fail, even with higher concentrations of the template RNA.
Lyo-Ready 1-Step Mix Competitor Mix
Retention of reverse transcriptase activity following lyophilization
Primers and probes were added to Lyo-Ready ™ 1-Step RT-qPCR Mix (Cat #MDX024), dried down and immediately rehydrated (purple) and tested against a freshly prepared wet mix (orange), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.
Lyophilised | Wet Mix
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