Ambient temperature stable qPCR Master Mixes

Saliva-Specific Mixes Over the last decade, saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection/logistics and similar to serum, saliva contains hormones, antibodies, growth factors, enzymes, and microbes that can be used as biomarkers for disease detection. However, there have been challenges adopting saliva for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors in saliva. Cumbersome sample preparation steps have been required to homogenize the specimen and adequately purify the DNA/RNA for molecular quantification. Meridian’s new Air-Dryable TM and Lyo-Ready TM direct saliva mixes are unique in that they have been designed to specifically overcome the inhibitors found in saliva or sputum samples. As a result, they are capable of highly sensitive amplification using crudely processed samples or extracted DNA or RNA in multiplex detection assays.

Saliva

PRODUCT

CAT NO. VOLUME REACTIONS

5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns

Air-Dryable Direct DNA qPCR Saliva Mixes

MDX130

Air-Dryable Direct RNA/ DNA qPCR Saliva Mixes

MDX131

Lyo-Ready Direct DNA qPCR Saliva Mixes

MDX132

Lyo-Ready Direct RNA/DNA qPCR Saliva Mixes

MDX133

Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or Universal Transport Media (up to 35% UTM)

Air-Dryable TM Direct DNA qPCR Saliva (MDX130)

Mycoplasma pneumoniae

ADV

CMV

0% human saliva | 10% human saliva | 20% human saliva | 60% human saliva

Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( black ), 10% ( red ), 20% ( blue ) and 60% ( grey ) homogenized human saliva. The results illustrate that the performance of the air-dried, Air-Dryable TM Direct DNA qPCR Saliva is not affected by increasing concentrations of human saliva.