NGS SOLUTIONS CATALOG
FRAGMENTATION
STEP 1
Lyophilized NGS Enzymatic DNA Fragmentation Kit
Cat. # MDX224 RXNS POUCHES 8 Fragmentation Mix
Lyophilized NGS Enzymatic DNA Fragmentation Kit (MDX224) is stable at ambient temperatures and can be rehydrated at the point of use, reducing handling steps and supporting consistent performance. Fragmentation variability can impact library quality and sequencing performance. MDX224 offers time-controlled fragmentation, allowing users to produce consistent and adjustable fragment sizes without mechanical shearing instruments.
Product Highlights • Ambient-temperature storage and global shipping • Reliable performance without freeze-thaw cycles • Consistent, time-controlled fragmentation across protocols • Ready-to-rehydrate format enables easy integration into existing workflows
Robust Time-Dependent Enzymatic Fragmentation
TIME (min)
AVG. SIZE (bp)
PEAK (bp)
8 12 15 Time (min)
8 min 12 min 15 min
8
900
960
12
580
562
15
450
143
Libraries were constructed from 1 µ g of human DNA. A fragmentation reaction time titration was conducted with MDX224, at 37°C for 8-, 12- and 15-minutes incubation. Progressive DNA fragmentation was observed, using a TapeStation (Agilent) with increasing incubation time, as indicated by the shift from high molecular weight genomic DNA to lower molecular weight fragments on the electropherograms. The changes in fragment size distribution and overall DNA integrity demonstrate time- dependent degradation of genomic DNA by MDX224.
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