Cardiac & Metabolic Markers Catalog

Cardiac & Metabolic Markers Reagents for Assay Development ISO Certified 13485:2016

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Company Overview

Extensive Capabilities & Services

Molecular Reagents qPCR | RT-qPCR | LAMP

Immuno Reagents Antigens | Antibodies | Blockers

ENZYMES • Hot-Start Taq technologies - chemical, antibody, aptamer • Lyo & Air-Dryable enzymes (glycerol free) Taq, Bst, RTase • Thermostable MMLV RT MASTER MIXES • Lyo & Air-Dryable formats • Inhibitor-tolerant mixes for stool, sputum, saliva, blood, plant, water. • For multiplexing, GC-rich templates

VIRUS MANUFACTURING • Live or inactivated • Proprietary Ag purification

RECOMBINANT PROTEINS •  E. coli, P. pastoris, S. cerevisiae , Sf9, Mammalian (CHO, HEK293) • 10L- 130L fermentation ANTIBODIES – MAbs/PAbs • 500+ MAbs produced in grams • Multi-Kilograms of MIgG / year • Hundreds of liters of GxhIgG • Ascites production (55,000 Mice)

NUCLEOTIDES • dNTPs, Na or Li salts • Ultra high purity, >99%

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Cardiac & Metabolic Markers for Assay Development

Commercial scale manufacturing of antigens and antibodies with protein purification expertise.

Meridian has been providing innovative life science solutions and building trusted partnerships for over 43 years. Meridian’s focus is to offer complete solutions for the development of molecular and immunological assays.

• Full line of immunoassay reagents, including antigens, antibodies and blockers • Large scale production of reagents for molecular assays • Technical support with assay development experience • Dedicated R&D and manufacturing teams • Robust and mature Quality System

ISO Certified 13485:2016

Global presence

WATERLOO, Belgium

LONDON, United Kingdom

QUEBEC, Canada

LUCKENWALDE, Germany

BILLERICA, MA

MANASQUAN, NJ

BEIJING, China

PARIS, France

CINCINNATI, OH (Headquarters)

CHANGZHOU, China

MILAN, Italy

MEMPHIS, TN

BOCA RATON, FL

MODIIN, Israel

SYDNEY, Australia

Diagnostic Manufacturing | Life Science Manufacturing | Sales & Warehouse

MERIDIAN BIOSCIENCE, INC. Parent Company | Founded in 1977 | Nasdaq: VIVO | 750+ Employees Headquartered in Cincinnati, OH | Presence in 70+ Countries.

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Company Overview

Antigens & Antibodies

INFECTIOUS DISEASE EXPERTISE

Gastro •  H. Pylori

Tropical • Zika • Dengue 1, 2, 3, 4 • Chikungunya

ToRCH & Childhood • Toxo • Rubella • CMV

Viral Hepatitis • HAV • HBV • HCV • HDV • HEV

•  C. Difficile • Norovirus • Adenovirus • Rotavirus

• Malaria • Chagas

• HSV-1,2 • Rubeola • EBV • Mumps • Coxsackie • Rotavirus • RSV • Parvo B19 • VZV

• Cryptosporidium • Campylobacter •  E. Coli

• Leishmaniasis • Leptospirosis • Newcastle Disease • Yellow Fever • Nipah Virus • JEV

• Salmonella •  G. Lambia • Astrovirus

STD • HSV-1, 2 • HIV-1, 2 • HPV • Syphilis • Chlamydia • Neisseria

Respiratory • SARS-CoV-2

•  M. Pneumoniae •  C. Pneumoniae • Influenza A, B • Parainfluenza •  L. Pneumophilia • RSV •  M. Tuberculosis • Streptococcus • Staphylococcus • Adenovirus

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Cardiac & Metabolic Markers for Assay Development

Cardiac • Troponin I, T • Myoglobin • BNP • NT-proBNP

Cancer • CA125 • CA15-3 • CA19-9 • CA72-4 • CA50 • CA242 • Cyfra 21-1 • CEA

Microbial Detection • Legionella • Salmonella • Cryptosporidium •  G. Lambia •  C. Jejuni •  E. Coli • Newcastle Disease • Canine Parvovirus • Rabies Virus • Serum Amyloid A (SAA) • Trichomonas foetus • Nipah • Transmissible Gastroenteritis Veterinary • ASFV • Avian Influenza • Borrelia •  Brucella abortus • Canine Distemper • Feline Immunodeficiency • Feline Leukemia • Foot-and-Mouth • Canine Heartworm • Infectious Bursal Disease • Marek Disease

Drug of Abuse • Amphetamine • Barbital • Benzodiazepine • Buprenorphine

• CRP • PCT • CK-MB • D-Dimer

• Cocaine • Cotinine • EDDP

• Fentanyl • Ketamine • K2 • MDMA (Ecstasy) • Methadone • Methamphetamine • Morphine • Norketamine • Opium • Oxycodone • PCP

• Cystatin-C • Galectin-3 • Vitamin D • Apo A, B, E • NSE • FABP • SAH • MPO • Fibrinogen • EGF

• Thyroglobulin • erbB-2/HER2 • AFP • EGFR

• HE4 • NSE • PMA • PAP • PSA

• PSMA • S-100 • PIVKA II • B2M

• Lp-PLA2 • PAPP-A

• Phenobarbital • Propoxyphene • THC

Hormones • LH, FSH, hCG, • hGH, AMH

Immunoglobulins/ Blockers • TRU Block ™ & IgM Diluent • Animal IgGs – Bovine, Chicken, Goat, Mouse, Rabbit, Sheep • Human IgA, IgG, IgM, IgE • Kappa Light chain • Lambda Light chain • Goat Anti-Human IgG, IgM, IgA • Goat Anti-Mouse IgG

Autoimmune • Jo-1 • PCNA

Allergens • Cat & Dog Allergen • Horse Allergen • Dust Mite • Alternaria alternate • Timothy Grass •  Platanus acerifolia • Mugwort • Cortisol • Estradiol • Insulin, C-peptide • Prolactin • Progesterone • PTH • PAPP-A • TSH, T3, T4, ACTH • Thyroglobulin

• pANCA • cANCA • Sm Ag • dsDNA • La(SSA) • Ro(SSA) • Histone • GMB • C1q • Scl-70 • SS-A • BS-Gly-1

•  B. Anthracis • Clostridium • Listeria

• Streptococcus • Staphylococcus

• Cathepsin G • Calprotectin

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Cardiac & Metabolic Diagnostics

Cardiovascular and metabolic diseases are the leading cause of death globally. An aggressive approach to their diagnosis and treatment can substantially reduce the risk of mortality and life-

threatening complications. Cardiovascular disease (CVD) is a major health problem across the world, accounting for a third of all deaths worldwide. It is often grouped with metabolic disorders because it is frequently a consequence of diabetes and dyslipidemia. However the group of disorders specifically related to CVD include coronary heart disease, cerebrovascular disease, and peripheral arterial disease. Cardiac biomarker assays have proven to be particularly useful in providing a rapid diagnosis and assessing risk in people with acute coronary syndrome (ACS). Cardiac markers are proteins, hormones, enzymes or other substances expressed by myocardial cells and they are released into the circulating blood upon cell necrosis. Several biomarkers that have become routinely used in the clinic include creatine kinase (CK), creatine kinase-MB

Retrieved 2013: www.cardiac-biomarkers.com

(CK-MB), myoglobin, cardiac troponin T (cTnT), and cardiac troponin I (cTnI). To date, the troponins have proven to be the most sensitive and specific indicators of cardiac injury. Other useful biomarkers include NT-proBNP which can help predict an increased risk of recurrent events after a heart arythmia. New potential cardiac biomarkers are continually being researched, and advances in functional genomics, proteomics, metabolomics, and bioinformatics have revolutionized the discovery process. In general, using more than one biomarker increases the early predictive value compared to using on a single marker and technological advances will increase the use of multi-marker profiling to improve and possibly individualize treatment of CVD in the future. Metabolic diseases include disorders that disrupt normal metabolism such as diabetes and dyslipidemia (abnormal lipid metabolism). These diseases can be brought on by primary (genetic) factors or secondary factors related to lifestyle, environment, or medication. Metabolic syndrome is a specific collection of conditions that can increase the risk of diabetes, stroke and heart disease and it affects 25% of the adult world population. In the past few years, several expert groups have attempted to set forth simple diagnostic criteria to be used in clinical practice to identify patients with metabolic syndrome. These risk factors include elevated waist circumference, elevated

triglycerides, reduced high-density lipoprotein cholesterol, elevated blood pressure and elevated fasting glucose. The magnitude of the increased risk can vary according to which components of the syndrome are present plus the other, non-metabolic syndrome risk factors in a particular person.

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Cardiac & Metabolic Markers for Assay Development

Product Quickfind List

A Adiponectin..................................... 5 Albumin. ......................................... 5 Angiotensin..................................... 6 Apolipoprotein A & B...................... 6 Apolipoprotein C............................. 7 Apolipoprotein E............................. 7 B BNP, ProBNP & NT-ProBNP........... 8 C Carboxy Methyl Lysine (CML) ....... 8 sCD40 Ligand (sCD40L)................. 9 Collagen Type I & III. ...................... 9 C-peptide........................................ 9 C-Reactive Protein (CRP)................ 9 Creatine Kinase BB (CK-BB)......... 10 Creatine Kinase MB (CK-MB)....... 10 Creatine Kinase MM (CK-MM)..... 10 Cystatin C..................................... 11 D D-Dimer........................................ 11 E Epidermal Growth Factor (EGF) .. 11 F Fatty Acid Binding Protein (FABP).12 Fibrinogen/ Fibrinopeptide A........ 12 G Galectin-3...................................... 12 Ghrelin. ......................................... 12 Glycogen Phosphorylase Isoenzyme BB (GPBB)................. 12 H Hemoglobin A1c (HbA1c)............. 13 I Interleukin 8 (IL-8)......................... 13

L Lectin-like Oxidized LDL

R Relaxin.......................................... 17 Renin............................................. 17 S S-100 ............................................ 18 S-Adenosyl-Homocysteine (SAH)..18 Serum Amyloid A......................... 18 Stromal Cell-Derived Factor 1 alpha (SDF-1 α ).............................. 18 T Thrombomodulin.......................... 19 Tissue Plasminogen Activator (tPA)............................... 19 Troponin Complex . ...................... 19 Troponin C-Cardiac (cTnC)............ 19 Troponin I-Cardiac (cTnI)............... 19 Troponin I-Skeletal (sTnI).............. 20 Troponin T-Cardiac (cTnT)............. 20 Tumor Necrosis Factor alpha (TNF-alpha).................................... 21 U Uroguanylin................................... 21 V Vitamin D...................................... 21

Receptor-1 (sLOX) Soluble Form. ..13 Lipoproteins HDL, LDL & VLDL... 13 Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)........ 14 Low Density Lipoprotein Receptor-Related Protein (LRP)... 14 M Malondialdehyde (MDA) . ............ 14 Monocyte Chemotactic Protein 1 (MCP-1/MCAF)............................. 14 Myeloperoxidase (MPO).............. 14 Myoglobin (MYO)......................... 15 N Neuron Specific Enolase (NSE).... 15 Neutrophil Gelatinase-Associated Lipocalin (NGAL)........................... 15 P Plasminogen Activator Inhibitor 1 (PAI-1).......................... 16 Pregnancy Associated Plasma Protein A (PAPP-A). ...................... 16 Procalcitonin (PCT). ...................... 16 Procollagen Type III...................... 17 Proinsulin...................................... 17

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Leading Cardiac Markers

Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP H86451M M Ab to NT-proBNP a.a. 61-76 (Capture) , ELISA & WB H86912M M Ab to NT-proBNP a.a. 1-12 (Detection) , ELISA & WB * Recognizes BNP, proBNP and NT-proBNP. K4A660R P Ab to BNP (Rabbit) ELISA, RIA, & IHC, lyophilized H01420M M Ab to NT-proBNP a.a. 61-76 ( Capture ), ELISA & WB H01419M M Ab to NT-proBNP a.a. 15-20 ( Detection ), ELISA & WB H01423M M Ab to NT-proBNP a.a. 15-20 ( Alternate Detection ), ELISA & WB * Detects NT-proBNP and proBNP. H01422M M Ab to NT-proBNP a.a. 5-12 (Capture) , ELISA & WB H01424M M Ab to NT-proBNP a.a. 22-36 (Detection) , ELISA & WB * Detects NT-proBNP regardless of glycosylation. Antibodies & Matched Pairs

D-dimer N01273M MAb ( Capture ), ELISA & WB N01274M MAb ( Detection ), ELISA & WB N01270M MAb (Capture) , ELISA, LF & WB N01269H MAb (Detection) , ELISA, LF & WB * Highly specific for D-dimer and other cross linked fibrin degradation products containing D-dimer, but no reactivity with Fragment D or E. No cross-reaction was found with purified intact fibrinogen or fibrinogen degradation products. N86304M MAb (Capture) , ELISA & WB N86352M MAb (Detection) , ELISA & WB * Reacts with high molecular weight fibrin degradation products. Does not cross react with fibrinogen or D-monomer.

Fatty Acid Binding Protein (FABP) H01324M

MAb (Capture) , ELISA & LF MAb (Detection) , ELISA & LF

H01323M

Fibrinogen N86413M MAb, ELISA & WB

Galectin-3 K01381M

C-Reactive Protein (CRP) M86005M MAb (Capture) , ELISA M01239M MAb (Detection) , ELISA * Recognizes antigen in the presence and in the absence of Ca 2+ .

MAb, ELISA * Reacts with human and mouse Galectin-3.

Glycogen Phosphorylase Isoenzyme BB (GPBB) H86807M MAb, ELISA & WB

Creatine Kinase (CK) MAC01-288 MAb to CK-BB, ELISA H31795M

Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) H01390M MAb (Capture) , ELISA H01393M MAb (Detection) , ELISA H01389M MAb (Alternate Detection) , ELISA H01392M MAb (Capture) , ELISA H01393M MAb (Detection) , ELISA

M Ab to CK-MB (Capture/Detection) , ELISA & LF P Ab (Goat) to CK-MM (Capture/Detection) , ELISA & LF

K31315G

* Abs can be used as capture or detection in a sandwich assay. MAC02-186 MAb to CK-MM, ELISA * 50% cross reactivity with CK-MB and < 1% cross reactivity with CK-BB. MAC02-512 MAb to CK-MM, ELISA * 52% cross reactivity with CK-MB, and no cross reactivity with CK-BB.

Myeloperoxidase (MPO) H87207M MAb, ELISA & IHC * Recognizes both native and recombinant human MPO.

Cystatin C H86023M H86218M H86013M

MAb (Capture) , ELISA MAb (Detection) , ELISA MAb (Alternate Detection)

Myoglobin (MYO) H01328M

MAb (Capture) , ELISA & LF MAb (Detection), ELISA & LF

H01327M

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Procalcitonin (PCT) E86561M

MAb (Capture) , ELISA & WB MAb (Detection) , ELISA & WB

E86420M

* Specific to procalcitonin and calcitonin. E86412M

MAb (Capture) , ELISA MAb (Detection) , ELISA

E86813M

* Specific for procalcitonin and katacalcin.

Troponin T-Cardiac (cTnT) H86429M

Pregnancy Associated Plasma Protein A (PAPP-A) E86141M MAb (Capture) , ELISA & WB E86910M MAb (Detection) , ELISA & WB * Detects both heterotetrameric (found in placental blood) and homodimeric (found in atherosclerotic plaques) PAPP-A. E86114M MAb, ELISA & WB S-Adenosyl-Homocysteine (SAH) K01395C PAb (Chicken), ELISA * Affinity purified, treated with Adenine-9--D-Ribofuranoside to remove cross reaction to Adenosine.

M Ab to a.a. 60-70 (Capture) , ELISA &WB M Ab to a.a. 95-181 (Detection) , ELISA & WB

H86111M

* Does not cross react with sTn.

Vitamin D K01214M

MAb to Vitamin D (25 OH), ELISA, LF,

CLIA & ELISA * Pairs with antigens A01697B & A01698B. K01213M

MAb to Vitamin D (25 OH), ELISA & RIA

* Pairs with antigens A01697B & A01698B. K24124M-LQ MAb to 25-OH Vitamin D3, ELISA K24124M MAb to 25-OH Vitamin D3, ELISA K24123M MAb to 1,25 (OH) 2 Vitamin D3, ELISA *Recognizes Native Human 25 OH Vitamin D2 and 25 OH Vitamin D3.

Soluble CD40 Ligand (sCD40L) P86106M MAb, ELISA P42374M MAb, FC & IFA

Troponin I-Cardiac (cTnI) H86285M

M Ab to a.a. 86-90 (Capture) , ELISA &WB

H01347M M Ab to a.a. 24-40 (Detection) , ELISA & WB * Reacts equally with free cTnI and complexed cTnI, does not cross react with sTn. H01340M MAb to a.a. 41-49, ELISA H86241M MAb to a.a. 87-91, ELISA &WB H86596M MAb to a.a. 169-178, ELISA &WB K31015M MAb (Capture) , ELISA K31341G P Ab to a.a. 27-39 (Goat, Detection) , ELISA, WB & IHC K31342G P Ab to a.a. 69-86 (Goat, Alternate Detection) , ELISA & IHC * Cross reactivity with sTn is < 0.1% (ELISA).

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Leading Cardiac Markers

Human Antigens (for use as standards, controls & calibrators)

Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP A24760H P roBNP, recombinant protein (E. coli) of N-terminal (a.a. 1-76, MW 8.39 kDa) A01261H N T-proBNP, recombinant protein (E. coli) of N-terminal (MW 8.589 kDa). Contains an additional methionine residue at the N-terminus (in comparison with native NT-proBNP) N86583H B NP and NT-proBNP Free Plasma, from

Lp-PLA2 A01413H Recombinant (E.coli) ,> 95% pure (SDS-PAGE) Myeloperoxidase (MPO) A38104H F rom human neutrophils, > 96% pure (SDS-PAGE), approximate activity of 1,100 Units/mg Protein Myoglobin A38131H From human heart, 99% pure (SDS-PAGE) Pregnancy Associated Plasma Protein A (PAPP-A) A86864H F rom human retroplacental blood.

normal human plasma, used for preparation of any proBNP-related standards and calibrators

A97201H F rom human fluids and reactive with monospecific goat anti-CRP, 95% pure (SDS-PAGE)

Heterotetrameric complex consisting of PAPP-A and pro-MBP subunits, > 85% pure (SDS-PAGE), lyophilized

Creatine Kinase (CK) VTI830

C K-BB, produced in cell culture (yeast), represents native full length protein and is enzymatically active C K-MB Isoenzyme Type I, produced in cell culture (yeast), enzymatically active CK-MB Isoenzyme Type II, produced in cell culture (yeast), represents native full length protein and is enzymatically active C K-MM Isoenzyme Type I, produced in cell culture (yeast), represents full length protein without the C-terminal lysine on both subunits and is enzymatically active C K-MM Isoenzyme Type III, produced in cell culture (yeast), represents full length protein and is enzymatically active

Procalcitonin (PCT) A01367H Recombinant protein (E. coli) representing a.a.

3-116 which corresponds to the unprocessed human protein, contains a 6-His tag at N-terminal

VTI840

VTI810

Troponin (cTnT & cTnI) A86813H c TnT, from human heart, > 98% pure (SDS-PAGE) A38150H c TnI, from human heart, > 98% pure (SDS-PAGE), single band ~24 kDa Vitamin D A01694D  25-OH Vitamin D3 BSA Conjugate, ≥ 99% Pure (HPLC) A01698B  Vitamin D Biotin Conjugate, > 95.7% Pure (HPLC) A01697B Vitamin D BSA Conjugate A50674H  Vitamin D Binding Protein (Gc-Globulin), > 95% Pure (SDS-PAGE) A01409H 1,25 Dihydroxy Vitamin D3, > 99% Pure (HPLC)

VTI850

VTI820

D-Dimer A01408H From human plasma, > 90% pure A01463H From human plasma, > 95% pure (SDS-PAGE) Fatty Acid Binding Protein (FABP) A86865H From human heart, > 95% pure (SDS-PAGE)

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Product list

Adiponectin A protein hormone that modulates a number of metabolic processes including glucose regulation and fatty acid oxidation. Low levels of adiponectin are associated with the increased prevalence of obesity-linked cardiovascular disease, including ischemic heart disease and peripheral arterial disease. Antibody Pairs

Abbreviations 6-His – Polyhistidine-tag

Aff.Pur. – Affinity Purified, analyte-specific column Alk.Phos. – Alkaline Phosphatase conjugated product C. Eggs – Chicken Eggs CLIA – Chemiluminescence Immunoassay CVD – Cardiovascular Disease DB – Dot Blot DFA – Direct Immunofluorescence Assay FC – Flow Cytometry FITC – Fluorescein conjugated product GST – Glutathione S-transferase HRP – Horseradish peroxidase conjugated product IB – Immunoblot

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION H01263M H01262M H01286M H01285M H01296M H01297M H01297M H01296M

* Detects total Adiponectin * Detects total Adiponectin * Detects total Adiponectin * Detects total Adiponectin

Antigens A01260H

• Native antigen from pooled human plasma • Affinity purified, > 95% pure (SDS-PAGE)

ICC – Immunocytochemistry IEP – Immunoelectrophoresis IFA – Immunofluorescence Assay

Albumin

IgG – Immunoglobin G IgM – Immunoglobin M IHC – Immunohistochemistry IP – Immunoprecipitation LF – Lateral Flow Lysate – Cells which have been lysed MAb – Monoclonal antibody Monospecific – Single band when tested by immunoelectrophoresis Neat – Whole, unpurified, undiluted antisera Neph – Product has been quality controlled by Nephelometry PAb – Polyclonal antibody Purified/IgG – Refer to the Certificate of Analysis regarding the extent of purification and the purification process used. RIA – Radioimmunoassay RID – Radial Immunodiffusion SDS-PAGE – Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis TIA – Product has been quality controlled by Turbidimetry WB – Western blot

Hypoalbuminemia is a medical condition in which blood levels of albumin are abnormally low. It is a common condition in patients with heart failure and becomes more prevalent with increasing age and illness. It can be used as an independent predictor of incident heart failure in end-stage renal disease and elderly patients. Monoclonals • Recognizes human serum albumin in ELISA • Produced in vivo H86424M Antibody Pairs • Suitable for use in ELISA, WB and the quantitative detection of human serum albumin in serum and urine • Does not react with bovine serum albumin or egg white albumin • MAbs produced in vivo CAPTURE DETECTION H86157M H86910M H86157M H86611M Antigens H8P01-767 • Native antigen from aphoresis plasma • Low B12 and low folate

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Product list | Continued

Angiotensin

Apolipoprotein A-II (Apo A-II) Apo A-II is the second most abundant protein of HDL particles and plays an important role in reverse cholesterol transport and lipid metabolism. Apo A-II is mainly produced in the liver and modulates lipoprotein lipase and hepatic triglyceride lipase. The Apo A-II test can be used as an aid in assessing the risk of CVD. Polyclonals CATALOG SOURCE FORMAT APPLICATION K45890G Goat Neat EIA,WB K97211R Rabbit Aff.Pur. EIA,IB G5C21-766 Goat Aff.Pur. IEP K34001G Goat Aff.Pur. EIA,WB K34004G Goat Biotin EIA,WB K74001P Goat HRP EIA,IB Apolipoprotein B (Apo B) Apo B is the primary apolipoprotein in LDL particles and high levels of Apo B and occurs in the plasma in two main isoforms, Apo B48 and Apo B100. Apo B48 is a unique protein to chylomicrons from the small intestine and Apo B100 is found in lipoproteins originating from the liver. It is well established that Apo B100 levels are associated with coronary heart disease, and serve as a better predictor of LDL levels. Monoclonals • Suitable for use in ELISA and RIA • Produced in vivo

Apolipoprotein A-I (Apo A-I) Apo A-I is the major protein component of high density lipoprotein (HDL) in plasma and plays a role in lipid metabolism. Monoclonals • Does not cross-react with Apo A-II or Apo B • Suitable for use in RIA and ELISA • Produced in vivo system, which is a major target for drugs that lower blood pressure. Angiotensin I is converted to Angiotensin II through the enzyme angiotensin-converting enzyme (ACE). ACE inhibitors are widely used in the treatment of arterial hypertension and cardiovascular diseases. Monoclonals • Suitable for use in ELISA • Produced in vivo E01296M (Angiotensin I) * Does not cross-react with II and III E01297M (Angiotensin II) * Cross-reacts with I and III Angiotensin is a peptide hormone that causes vasoconstriction leading to high blood pressure. It is part of the renin-angiotensin

MAC20-001 MAC20-029 H45402M H45404M H45625M Polyclonals CATALOG

MAC23-031 MAC23-035 H45160M H45161M H45640M Polyclonals CATALOG

SOURCE FORMAT

APPLICATION

K45252G Goat K45252P Goat K01234G Goat K23600G Goat K04329G Goat

Aff.Pur.

EIA,WB EIA,WB

HRP

Monospecific IEP

Neat

RID

Monospecific IEP

SOURCE FORMAT

APPLICATION

* K97110S is specific for ApoA only (not ApoA I)

Apo B G5C23-766 Goat K01235G Goat K04323G Goat K62200G Goat

Aff.Pur.

N/A

Monospecific IEP Monospecific IEP

Monospecific EIA,IEP,TIA

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Apolipoprotein B (Apo B) continued

Apolipoprotein CIII

CATALOG

SOURCE FORMAT

APPLICATION

A protein that regulates both lipoprotein lipase and lecithin- cholesterol acyltransferase and may cause hypertriglyceridemia. Plasma levels of lipoprotein Apo CIII predict coronary heart disease and are associated with metabolic syndrome. Polyclonals CATALOG SOURCE FORMAT APPLICATION K74130G Goat Neat EIA,IB K74140G Goat Aff.Pur. EIA,IB K74140P Goat HRP EIA,IB K74170B Goat Biotin EIA,IB K97113R Rabbit Aff.Pur. EIA,IB Antigens A34129H • Native antigen from human plasma (VLDL) • 95% pure (SDS PAGE) Apolipoprotein E (Apo E) A major component of very low-density lipoproteins (VLDLs) and binds to a specific receptor on liver and peripheral cells. There are 3 major alleles: E3, E4, and E2. E3 is the normal form with normal funciton, the E2 variant has a reduced ability bind receptors and is associated with a high risk of CVD and the E4 variant is associated with increased levels of LDL- cholesterol and decreased levels of HDL-cholesterol, which is associated with a higher risk of developing coronary artery disease. Monoclonals

Apo B–48/100 K45253G Goat K34003G Goat K34005G Goat

Aff.Pur.

EIA,WB EIA,WB EIA,WB

Biotin

HRP

Antigens A34300H

• Native Apo B100 from human plasma LDL • Cross-reacts with anti-LDL

Apolipoprotein AI/B (Apo AI/B) Calibrator

Antigens A62934H

• Native antigen from human plasma • Apo AI: 313.0 mg/dL (lot dependent) • Apo B: 307.0 mg/dL (lot dependent)

Apolipoprotein CII (Apo CII)

Apo CII acts as a co-factor for lipoprotein lipase, an enzyme that hydrolyses triglycerides in chylomicrons and VLDL. Can be used as an aid in assessing the risk of cardiovascular disease. Polyclonals CATALOG SOURCE FORMAT APPLICATION K74322G Goat Neat EIA,IB K97112R Rabbit Aff.Pur. EIA,IB Antigens A50302H • Native antigen from human plasma VLDL • 95% pure (SDS-PAGE)

• Reacts with VLDL of human plasma • Suitable for use in ELISA and IHC • Produced in vivo , lyophilized H61529M Polyclonals CATALOG

SOURCE FORMAT APPLICATION

G5C27-766 Goat K34002G Goat K74190G Goat K74180B Goat

Aff.Pur.

N/A

HRP

EIA,WB EIA,WB EIA,WB

Aff.Pur.

Biotin

Antigens A50120H

• Native antigen from human plasma (VLDL) • > 95% pure (SDS-PAGE)

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Product list | Continued

Antigens R01614

BNP, ProBNP & NT-ProBNP BNP (B type natriuretic peptide) is a hormone and important biomarker with an established role in the diagnosis of congestive heart failure. Its utility has also been explored in myocardial ischemia and infarction, and in acute pulmonary embolism. When BNP is secreted it is attached to a 76–amino acid N-terminal fragment called NT-proBNP which is biologically inactive. Once released, BNP binds to and activates the atrial natriuretic factor (ANP) receptors. Monoclonals • Reacts with proBNP, N-terminal • Suitable for proBNP and NT-proBNP immunoassay H86705M a.a. 13-27 H86010M a.a. 28-45 *Also works in WB H86916M a.a. 46-60 *Also works in WB H86288M a.a. 61-76 Antibody Pairs • Suitable for use in ELISA and WB • Specific for Human BNP unless otherwise specified CAPTURE DETECTION H86051M H86262M

• proBNP recombinant (E. coli) • Suitable for enzyme linked fluorescent assay • 95% pure (Silver Staining SDS-PAGE)

A24108H

• proBNP recombinant (E. coli) • > 95% pure (SDS-PAGE)

A01455H

• proBNP recombinant (E. coli) • Suitable for use in ELISA as a calibrator, standard or an immunogen for antibody development • Contains one additional Methionine residue at the N-Terminus (in comparison with native proBNP) • > 95% pure (Tricine SDS-PAGE)

A24810H

• proBNP, C-terminal (synthetic) • Represents amino acids 1-32 • > 95% pure (HPLC), lyophilized

R01616

• NT-proBNP recombinant (E. coli) • > 95% pure (Silver Staining SDS-PAGE) • Suitable for enzyme linked fluorescent assay

A01261H

• NT-proBNP recombinant (E. coli) • Suitable for use in ELISA • > 95% pure (Tricine SDS-PAGE)

H86051M H01365M H01365M H86507M H01365M H86573M H01365M H01366M H86051M H86915M

A24607H

• proBNP, N-terminal recombinant (synthetic) • Represents a.a.1-76 • > 95% pure (HPLC), lyophilized • proBNP, N-terminal recombinant (E. coli) • Represents the N-terminal a.a.1-76 • > 95% pure (SDS-PAGE), lyophilized

*Recognizes BNP & pro-BNP

*Recognizes pro-BNP

H86451M

H86132M

*Recognizes proBNP & NT-proBNP

A24760H

H86451M H86912M H86451M H86915M H86511M H86214M H01420M H01422M H01420M H01419M H01420M H01423M

*Recognizes proBNP & NT-proBNP

*Recognizes proBNP & NT-proBNP

*Recognizes proBNP & NT-proBNP

*Detects NT-proBNP and proBNP

Carboxy Methyl Lysine (CML) A well-characterized glycoxidation product, formed from the oxidation of both carbohydrates and lipids, which accumulates in tissues with age. CML is a biomarker of general oxidative stress which is involved in the pathogenesis of atherosclerosis. Polyclonals CATALOG SOURCE FORMAT APPLICATION K97135G Goat Aff. Pur. EIA,WB

*Detects NT-proBNP and proBNP

*Detects NT-proBNP and proBNP

Polyclonals CATALOG

SOURCE FORMAT APPLICATION

K4A660R Rabbit K4A670R Rabbit K24410R Rabbit

Neat

EIA,IFA,IHC,RIA

Purified

EIA,IHC,RIA

Neat

RIA

8

8 | Cardiac & Metabolic Markers for Assay Development Cardiac & Metabolic Markers for Assay D velopment

Monoclonals • Reacts with human free C-peptide and Proinsulin • Suitable for use in ELISA and RIA • Produced in vivo E86588M • Reacts with C-peptide • Suitable for use in ELISA and RIA E86710M MHP18-601 MHP18-6014 * Biotin conjugated

sCD40 Ligand (sCD40L)

A member of the TNF superfamily primarily expressed on activated T cells. Studies suggest that sCD40L may be at the heart of the atherosclerotic process, specifically playing a role in the inflammatory aspects of atherosclerotic lesion progression, thrombosis and restenosis. Monoclonals • Suitable for use in ELISA and WB • C an be used for detection of human sCD40L in serum P86210M

Collagen Type I & III

C-Reactive Protein (CRP)

Increased myocardial collagen accumulation is present in almost every cardiac disease and plays an important role in the reduced heart function. N-terminal and C-terminal propeptides of Collagen Type I and III are the two major collagen types in the heart and reflect collagen synthesis and degradation. Their role as serum biomarkers for the diagnosis of cardiac fibrosis is being studied. Monoclonals • Specific for both native and heat denatured human Collagen Type I • Suitable for ELISA and IHC M1A340M Polyclonals CATALOG SOURCE FORMAT APPLICATION Collagen Type III T40233R Rabbit Purified EIA,IFA,IHC Antigens A33123H • Native Human Collagen Type III • Sourced from placental villi, > 90% pure (SDS-PAGE) • Suitable as a immunoassay standard and for antibody production

CRP is mainly used as a maker of inflammation. High- sensitivity CRP assays can be used for determining the risk of cardiovascular disease, heart attacks, and stroke. Antibody Pairs • Suitable for use in ELISA, high sensitivity • MAbs produced in vivo CAPTURE DETECTION M86005M M01239M * Recognizes CRP with or without Ca2+. M01243M M01239M * Recognizes CRP with or without Ca2+. M01331M M01330M Monoclonals • Recognizes CRP with or without the presence of Ca 2+ • Suitable for use in ELISA • Produced in vivo M01238M * Can also be used in IHC and WB

M66112M M66113M M86007M M86842B

* Can also be used in IHC

* Biotin conjugated

M86842M • Recognizes CRP • Suitable for use in ELISA (Unless otherwise specified) • Produced in vivo M01319B * Biotin conjugated

C-peptide & Proinsulin

M01319M M01295M

C-peptide is a combination of amino acids that are a by-product of insulin and the level of this protein indicates how much insulin the pancreas is producing. Studies have shown an association between serum C-peptide levels and risk of cardiovascular-related disease and coronary artery-related mortality among adults without diabetes.

* No applications have been tested

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Product list | Continued

C-Reactive Protein (CRP) continued

Creatine Kinase MB (CK-MB)

Polyclonals CATALOG

CK-MB is expressed in heart muscle and it is used in the diagnosis of acute myocardial infarction and possible reinfarction. The half- life of CK-MB in the circulation is relatively short (approximately 12 hours). CK-MB is relatively cardiac-specific, even healthy people may have low concentrations of this isoenzyme in their blood. Antibody Pairs • Suitable for use in ELISA and LF • MAbs produced in vivo CAPTURE DETECTION H31795M K31315G * K31315G is a polyclonal Goat anti-CK-MM K31315G H31795M * K31315G is a polyclonal Goat anti-CK-MM H01330M H01329M Antigens VTI840 • Recombinant (P. pastoris) , Isoenzyme Type I • Suitable for use in ELISA VTI810 • Recombinant (P. pastoris) , Isoenzyme Type I • Suitable for use in ELISA

SOURCE FORMAT APPLICATION

L01237G Goat M01333C C. Egg

Purified Aff. Pur.

IEP N/A

Antigens A01430H

• Native antigen from human fluids • 98% pure (SDS-PAGE)

A01250H

• Recombinant (E. coli) • Pentamer structure

Creatine Kinase BB (CK-BB)

CK-BB is found mostly in the brain and increases in response to brain injury, meningitis, abnormal cell growth, severe shock, stroke, hypothermia, or restricted blood flow to the bowel. It is used in creatine kinase test which determines what percentage of the total creatine kinase is due to the type produced by damaged heart muscles (CK-MB). Monoclonals • Produced in vivo MAC01-288 Antigens VTI830 • Recombinant (P. pastoris) • Full length protein with amino acid sequence identical to the native enzyme

Creatine Kinase MM (CK-MM)

Found in your skeletal muscle and heart. CK-MM generally rises if you have muscle damage in your heart, brain, or skeleton after a crush injury, seizures, muscular dystrophy, muscle inflammation, or another skeletal muscle disorder. It is used in creatine kinase test which determines what percentage of the total creatine kinase is due to the type produced by damaged heart muscles (CK-MB). Monoclonals • Suitable for use in ELISA • Produced in vivo

• > 95% pure (SDS-PAGE) • Suitable for use in ELISA H6C01-323 • Native antigen from human brain

• 52% cross-reactivity to CK-MB • 0% Cross-reactivity to CK-BB

• Activity: 730 Units/mL at 37ºC (lot dependent)

MAC02-186 MAC02-512 Polyclonals CATALOG

SOURCE FORMAT

APPLICATION

G5C02-766 Goat H01310G Goat K31315G Goat

Aff.Pur. Purified Aff.Pur.

N/A N/A

EIA,LF

* K31315G pairs with MAb to CK-MB H31795M

10

10 | Cardiac & Metabolic Markers for Assay Development r ia t lic rk rs f r s y v lo t

Antibody Pairs • Suitable for use in ELISA and WB • Reactive with D-Dimer and other fribin degradation products • MAbs produced in vivo CAPTURE DETECTION N01270M N01269H * Does not cross-react Fragment D or E, also suitable for LF N01273M N01274M * Does not cross-react with Fibrinogen

Creatine Kinase MM (CK-MM) continued

Antigens VTI850

• Recombinant (P. pastoris) , Isoenzyme Type I • Full length protein without the C-terminal lysine on both subunits • Suitable for use in WB and ELISA • Recombinant (P. pastoris) , Isoenzyme Type I • Identical amino acid sequence to native protein • Suitable for use in ELISA

VTI820

N86304M N86925M N86304M N86043M Antigens A01408H

A38925H

• Native antigen from human heart, lyophilized

• Native antigen from human plasma • > 90% pure (SDS-PAGE and gel-scanning) • Native antigen from human plasma • Suitable for ELFA • 95% pure (SDS-PAGE) • Native antigen from human plasma • > 90% pure (SDS-PAGE and gel-scanning), lyophilized

Cystatin C

A01463H

Traditionally used as a biomarker of kidney function, it has recently been studied for its role in predicting new onset or deteriorating CVD. In different cardiovascular conditions, Cystatin C is a sensitive marker for renal dysfunction, a predictor of the development of heart failure, and is independently associated with subsequent CVD and outcome. Antibody Pairs

A86870H

Epidermal Growth Factor (EGF) EGF is a growth factor with potential use as cardiac biomarker due to its role in the proliferation of cardiac stem cells. Research suggests that the human heart has an endogenous reserve of cardiac stem cells (CSCs) which can be activated by various growth factors to reconstitute injured myocardium. EGF has shown to significantly promote the proliferation, migration, and wound healing activities of these CSCs in comparison to the other growth factors. Antigens VTI880 • Recombinant (P. pastoris)

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION H86023M H86013M Antigens A01419H

• Native antigen from human urine

D-Dimer

D-dimer is a marker of fibrin turnover and elevated levels are indicative of the presence of a clot and have been reported in deep vein thrombosis, pulmonary embolism, disseminated intravascular coagulation, acute aortic dissection, myocardial infarction, malignant diseases, obstetrical complications, third trimester of pregnancy, and surgery. It is a marker for the early diagnosis of acute coronary syndromes and can be used in addition to traditional assessment for myocardial infarction. Monoclonals • D oes not cross-react with fibrinogen and D-monomer (unless noted otherwise) • S uitable for use in ELISA and WB N86925M * Reacts with high molecular weight fibrin degradation products N86925B * Biotin conjugated

• Suitable for use in WB • >95% pure (SDS-PAGE)

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Fatty Acid Binding Protein (FABP)

Galectin-3

Heart type FABP is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. It is an early biomarker for myocardial infarction, detected in the blood within one to three hours of acute coronary syndrome. Antibody Pairs

Galectin-3 is a galactoside-binding protein G that binds to and activates myofibroblasts in damaged heart tissue, leading to collagen synthesis and subsequently problematic fibrosis. It has been correlated with the prognosis in patients with coronary heart failure and the first Galectin-3 biomarker assay was approved by the FDA in 2010. Monoclonals K01381M *Suitable for use in FC, produced in vivo K01382M *Suitable for use in FC, produced in cell culture

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE H86505M H01314M H86101M H01314M H01315M H01324M H01323M H86101M

DETECTION

Ghrelin

* Also works in LF assays

Antigens A86865H

Clinical studies suggest a role for ghrelin in the treatment of CHF. Known cardiovascular effects of ghrelin include lowering of peripheral resistance either direct at the vascular level, and/ or by modulating sympathetic nervous activity. Gherlin also has potential as a marker of abnormal metabolism in cardiac failure. Monoclonals • Suitable for use in ELISA • Produced in vivo E86621M Glycogen Phosphorylase Isoenzyme BB (GPBB) GPBB is an isoenzyme of glycogen phosphorylase and a key enzyme of glycogenolysis. It has been suggested as an early marker of acute myocardial infarction and unstable angina. Monoclonals • Suitable for use in ELISA and WB • Produced in vivo H86807M

• Native antigen from human heart • Suitable for use as a immunoassay standard, immunogen for antisera production, or a tracer for iodination • > 95% pure (10-20% gradient SDS-PAGE) • FABP3 Recombinant (E.coli) • Suitable for use in ELISA • 95% pure (silver staining SDS-PAGE)

R01617

Fibrinogen/ Fibrinopeptide A

Fibrinogen is a plasma glycoprotein that is essential for blood blotting and has the ability to promote thromboses, or clots, by causing platelets to clump inside blood vessels. A number of studies have shown elevated fibrinogen levels to be a major risk factor for coronary heart disease. Antibody Pairs • Suitable for use in ELISA

• For specific fibrinogen detection, pair a capture anti-fibrinopeptide A antibody with a detection anti-fibrinogen antibody • MAbs produced in vivo CAPTURE DETECTION N86710M N86413M * N86710M is specific for Fibrinopeptide A

12

12 | Cardiac & Metabolic Markers for Assay Development ardiac etabolic arkers for say evelop ent

Lipoproteins HDL, LDL, VLDL Receptor Lipoprotein diagnostics, such as LDL cholesterol and HDL cholesterol, help to diagnose dyslipidemia which is an important risk factor for cardiovascular disease and type II diabetes. Monoclonals • MAb to LDL Receptor-Related Protein (LRP) • Will not block ligand binding • Suitable for use in ELISA, WB, IHC, and FC

Hemoglobin A1c (HbA1c) As glucose circulates in the blood, some of it spontaneously binds to hemoglobin to form hemoglobin A1c (HbA1c). The development of specific diabetes complications correlates with glycated haemoglobin (HbA1c), the most accepted measure of chronic glycaemia. Monoclonals

• Suitable for use in sandwich ELISA • Does not cross-react with HbA10 • Produced in vivo H01331M

H63650M Antigens A95132H

* Can also work in indirect ELISA (antigen capture)

• Native HDL from human normolipidemic plasma • Devoid of VLDL, IDL and LDL • Ultracentrifugation at density 1.063–1.210 • Native HDL 2 (HDL2) Subfraction from human normolipidemic plasma • Devoid of VLDL, IDL, LDL, and HDL3 • Ultracentrifugation density at 1.063 - 1.125 • Native HDL 3 (HDL3) Subfraction from human normolipidemic plasma • Devoid of VLDL, IDL, LDL, and HDL2 • Ultracentrifugation density at 1.120–1.21g/mL • Reconstituted HDL from human plasma • A complex of human Apolipoprotein A-I and 1-palmitoyl-2-oleoyl phosphatidylcholine prepared at a molar ratio of 1 to 100 by the cholate removal method

H01388M

* Can also work in WB

Antigens A01273H

• Native antigen from human erythrocytes • > 96% HbAlc (HPLC analysis using a Mono S Column)

A95322H

Interleukin 8 (IL-8) IL-8 is a neutrophil chemokine produced by macrophages, lymphocytes, epithelial cells and endothelial cells, and promotes neutrophil chemotaxis. Studies in patients with CAD have demonstrated that IL-8 may predict cardiovascular events independent of the other cytokines and high sensitivity CRP. Antigens A42280H • Recombinant (E. coli), a.a. 1-72 • > 98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE) A42208H • Recombinant (E. coli) , a.a. 1-77 • > 98% pure (HPLP and SDS-PAGE)

A95332H

A34275H

A34013H

• Native VLDL from human plasma • Devoid of IDL, LDL, and HDL • Purification by ultracentrifugation at density 1.006

Lectin-like Oxidized LDL Receptor-1 (sLOX) Soluble Form

A major endothelial receptor for oxidized low-density lipoprotein which is believed to play a role in atherosclerosis. Its expression is induced in pro-atherogenic settings including hypertension, hyperlipidemia, and diabetes, and it accumulates in therosclerotic lesions where it has been strongly linked to plaque rupture. Specifically sLOX-1 levels are elevated in acute coronary syndrome at an early stage, suggesting its usefulness as an early diagnostic marker of acute coronary syndrome. Monoclonals • Specific for human recombinant sLOX-1 (a.a. 58-273) • Suitable for use in ELISA and WB • Produced in vivo H86922M

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Product list | Continued

Malondialdehyde (MDA)

Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Lp-PLA2 is a protein produced by inflammatory cells that circulates mainly with LDL and is responsible for hydrolyzing oxidized phospholipids in LDL. It is highly upregulated in atherosclerotic plaques and is thought to be directly involved in the development of atherosclerosis and plaque rupture. Monoclonals

MDA is a reactive aldehyde produced by the degradation of polyunsaturated lipids and causes oxidative modification of LDL to form malondialdehyde low density lipoprotein (MDA- LDL). MDL-LDL is a sensitive biomarker for ACS patients with unstable angina and acute myocardial infarction. MDA-LDL not only serves as an oxidative stress marker but as a marker of plaque destabilization. Polyclonals CATALOG SOURCE FORMAT APPLICATION K97120P Goat HRP, Lyophilized EIA,WB

• Suitable for use in ELISA • Produced in cell culture H01389M Antibody Pairs • Suitable for use in ELISA • Produced in cell culture CAPTURE DETECTION H01390M H01393M H01390M H01389M H01392M H01393M Polyclonals CATALOG

Monocyte Chemotactic Protein 1 (MCP-1/MCAF)

Myeloperoxidase (MPO) MPO is a peroxidase enzyme and a marker of inflammation and neutrophil activation during strenuous exercise. In addition, it has been implicated in the oxidation of lipids contained in low-density lipoproteins, infiltration of macrophages and neutrophils, formation of unstable coronary plaques, and plaque rupture. MPO is elevated in patients with coronary artery disease and can serve as a prognostic marker to identify patients with unstable plaques before complete microvascular obstruction. Monoclonals • Suitable for use in ELISA • Produced in vivo (unless stated otherwise) H87207M *Also works in WB, IHC, FC and produced in cell culture K86005M *Also works in WB Specifically, high levels of MCP-1 have been associated with a poor prognosis and increased risk for death independent of other risk factors in patients with ACS. Antigens A01251H • Recombinant (E. coli) • Non-glycosylated polypeptide chain containing 76 a.a. • > 98% pure (RP-HPLC and SDS-PAGE), lyophilized MCP-1 is a cytokine produced by macrophages, smooth muscle cells and endothelial cells within atherosclerotic plaques and an important indicator of atherosclerotic plaque burden.

SOURCE FORMAT APPLICATION

H01333S Sheep

Aff.Pur.

EIA,IHC,WB

Antigens A01413H

• Recombinant (E. coli) • > 95% pure (SDS-PAGE) • Contains a His-tag

Low Density Lipoprotein Receptor-Related Protein (LRP) LRP is a large multifunctional receptor mediating the clearance of diverse ligands, including selected lipoproteins, various protease inhibitor complexes, and thrombospondin. The range of ligands recognized by LRP suggests that it plays a role in diverse processes including lipid metabolism, cell growth, migration, and tissue invasion. Monoclonals • Suitable for use in WB, IHC, and FC • Produced in vivo H63180M * Specifically reacts with the 515 kDa alpha-chain of human LRP/alpha2 H63080M * Specific for human light chain of LRP, 85 kDa

14

14 | Cardiac & Metabolic Markers for Assay Development Cardiac & Metabolic Markers for A say Develop ent

Myeloperoxidase (MPO) continued

Neuron Specific Enolase (NSE) NSE is one of three recognized forms of enolase, an enzyme in the glycolysis pathway. Studies have shown NSE concentrations rise in the cerebrospinal fluid following an ischemic stroke. Antibody Pairs • Specific for the gamma subunit without detectable cross- reactivity with the alpha or beta subunits

Antigens A01462H

• Native antigen from human neutrophils • Suitable for use in ELISA • Native antigen from human neutrophils • > 96% pure (SDS-PAGE) • Native antigen from human neutrophils • 95% pure (SDS-PAGE), lyophilized

A38104H

A50181H

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION M86520M M86101M M86406M M86101M M86416M M86101M M86141M M86201M M86101M M86520M Antigens A86803H

Myoglobin (MYO) Myoglobin is the primary oxygen-carrying pigment of muscle tissue and it is present in high levels in serum when muscle tissue is damaged, such as after a heart attack. Myoglobin achieves its maximal diagnostic sensitivity within 5 hours of symptom onset and is an early marker of acute myocardial infarction. However, in patients with concurrent trauma or renal failure it exhibits poor specificity. Antibody Pairs • Suitable for use in ELISA • MAbs produced in vivo (unless noted otherwise) CAPTURE DETECTION

• Native antigen from human brain • > 95% pure (SDS-PAGE) • Suitable for use in ELISA and WB

A01359H

• Recombinant (E. coli) , a.a. 2-434 • > 95% pure (SDS-PAGE) • Suitable for use in ELISA, LF, WB, DB and IHC

H86142M H86703M H86703M H86142M Polyclonals CATALOG H01370C C. Eggs

A01448H

• Recombinant (E. coli) , a.a. 1-434 • 95% pure (SDS-PAGE) • Suitable for use in ELISA

SOURCE FORMAT APPLICATION

Aff.Pur.

N/A

Neutrophil Gelatinase-Associated Lipocalin (NGAL) / Lipocalin-2

Antigens A38131H

• Native antigen from human heart tissue • 99% pure (SDS-PAGE, reduced) • Native antigen from human heart tissue • > 95% pure (SDS-PAGE), lyohphilized • Suitable for use as a reference or standard in immunoassays

A glycoprotein involved in the regulation of cellular apoptosis and the transport of iron across cell membranes. It has recently been implicated in atherosclerosis through its effects on MMP-9, an important mediator of vascular remodelling and plaque instability. Increased levels of NGAL have been found in patients with atherosclerotic plaques and myocardial infarction. Polyclonals CATALOG SOURCE FORMAT APPLICATION K01392C C. Eggs Aff.Pur. N/A Antigens 9278

A86850H

• Recombinant (CHO cells) • 95% pure (SDS-PAGE) • Suitable for use in WB

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