Cardiac & Metabolic Markers Catalog

Cardiac & Metabolic Markers for Assay Development ISO Certified

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Cardiac & Metabolic Diagnostics

Cardiovascular and metabolic diseases are the leading cause of death globally. An aggressive approach to their diagnosis and treatment can substantially reduce the risk of mortality and life-threatening complications.

Cardiovascular disease (CVD) is a major health problem across the world, accounting for a third of all deaths worldwide. It is often grouped with metabolic disorders because it is frequently a consequence of diabetes and dyslipidemia. However the group of disorders specifically related to CVD include coronary heart disease, cerebrovascular disease, and peripheral arterial disease. Cardiac biomarker assays have proven to be particularly useful in providing a rapid diagnosis and assessing risk in people with acute coronary syndrome (ACS). Cardiac markers are proteins, hormones, enzymes or other substances expressed by myocardial cells and they are released into the circulating blood upon cell necrosis. Several biomarkers that have become routinely used in the clinic

Retrieved 2013: www.cardiac-biomarkers.com

include creatine kinase (CK), creatine kinase-MB (CK-MB), myoglobin, cardiac troponin T (cTnT), and cardiac troponin I (cTnI). To date, the troponins have proven to be the most sensitive and specific indicators of cardiac injury. Other useful biomarkers include NT-proBNP which can help predict an increased risk of recurrent events after a heart arythmia. New potential cardiac biomarkers are continually being researched, and advances in functional genomics, proteomics, metabolomics, and bioinformatics have revolutionized the discovery process. In general, using more than one biomarker increases the early predictive value compared to using on a single marker and technological advances will increase the use of multi-marker profiling to improve and possibly individualize treatment of CVD in the future. Metabolic diseases include disorders that disrupt normal metabolism such as diabetes and dyslipidemia (abnormal lipid metabolism). These diseases can be brought on by primary (genetic) factors or secondary factors related to lifestyle, environment, or medication. Metabolic syndrome is a specific collection of conditions that can increase the

risk of diabetes, stroke and heart disease and it affects 25% of the adult world population. In the past few years, several expert groups have attempted to set forth simple diagnostic criteria to be used in clinical practice to identify patients with metabolic syndrome. These risk factors include elevated waist circumference, elevated triglycerides, reduced high-density lipoprotein cholesterol, elevated blood pressure and elevated fasting glucose. The magnitude of the increased risk can vary according to which components of the syndrome are present plus the other, non-metabolic syndrome risk factors in a particular person.

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Cardiac & Metabolic Markers for Assay Development

Product Quickfind List

A Adiponectin..................................... 8 Albumin. ......................................... 8 Angiotensin..................................... 9 Apolipoprotein A & B...................... 9 Apolipoprotein C........................... 10 Apolipoprotein E........................... 10 B BNP, ProBNP & NT-ProBNP......... 11 C Carboxy Methyl Lysine (CML) ..... 12 sCD40 Ligand (sCD40L)............... 12 Collagen Type I & III. .................... 12 C-peptide...................................... 12 C-Reactive Protein (CRP).............. 12 Creatine Kinase BB (CK-BB)......... 13 Creatine Kinase MB (CK-MB)....... 13 Creatine Kinase MM (CK-MM)..... 13 CX3CR1........................................ 14 Cystatin C..................................... 14 D D-Dimer........................................ 14 E Endothelial Nitric Oxide Synthase (eNOS).......................... 15 Endothelin-1.................................. 15 Epidermal Growth Factor (EGF) .. 15 F Fatty Acid Binding Protein (FABP).15 Fibrinogen/ Fibrinopeptide A........ 16 G Galectin-3...................................... 16 Ghrelin. ......................................... 16 Glycogen Phosphorylase Isoenzyme BB (GPBB)................. 16 H Hemoglobin A1c (HbA1c)............. 17 I Interleukin 8 (IL-8)......................... 17

L Lectin-like Oxidized LDL

R Relaxin.......................................... 22 Renin............................................. 22 S S-100 ............................................ 23 S-Adenosyl-Homocysteine (SAH)..23 Serum Amyloid A......................... 23 Stromal Cell-Derived Factor 1 alpha (SDF-1 α ).............................. 23 T Thrombin. ..................................... 24 Thrombospondin ......................... 24 Tissue Plasminogen Activator (tPA)............................... 24 Troponin Complex . ...................... 24 Troponin C-Cardiac (cTnC)............ 24 Troponin I-Cardiac (cTnI)............... 24 Troponin I-Skeletal (sTnI).............. 25 Troponin T-Cardiac (cTnT)............. 26 Tumor Necrosis Factor alpha (TNF-alpha).................................... 26 U Uroguanylin................................... 26 V Vitamin D...................................... 26 Von Willebrand Factor (VEF)......... 27

Receptor-1 (sLOX) Soluble Form. ..17 Leptin............................................ 17 Lipoproteins HDL, LDL & VLDL... 17 Lipoprotein-Associated Phospholipase A2 (Lp-PLA2)........ 18 Low Density Lipoprotein Receptor-Related Protein (LRP)... 19 M Malondialdehyde (MDA) . ............ 19 Monocyte Chemotactic Protein 1 (MCP-1/MCAF)............................. 19 Myeloperoxidase (MPO).............. 19 Myoglobin (MYO)......................... 19 N Neuron Specific Enolase (NSE).... 20 Neutrophil Gelatinase-Associated Lipocalin (NGAL)........................... 20 P Plasminogen Activator Inhibitor 1 (PAI-1). .......................................... 20 Platelet Factor IV (PF-IV)............... 21 Pregnancy Associated Plasma Protein A (PAPP-A). ...................... 21 Procalcitonin (PCT). ...................... 21 Procollagen Type III...................... 22 Proinsulin...................................... 22

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Company Overview

Meridian Life Science, Inc. is a leading large scale manufacturer of: • Antibodies • Viral antigens • Recombinant proteins • PCR enzymes • Nucleotides • Critical assay reagents Meridian has been providing innovative life science solutions and building trusted partnerships for over 40 years. Meridian’s focus is to offer products and services that help to advance the development of diagnostic assays and vaccine development. • Commercial scale manufacturing of antigens and antibodies with protein purification expertise • Full line of immunoassay reagents, including antigens, antibodies and blockers

• Large scale production of reagents for molecular assays • Technical support with assay development experience • Dedicated R&D and manufacturing teams • Robust and mature Quality System ISO certified

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Cardiac & Metabolic Markers for Assay Development

Extensive Capabilities and Services Immunodiagnostics • Antigens & Antibodies • Recombinant Proteins • Blocking reagents Molecular Diagnostics • Nucleotides • enzymes • qPCR/PCR reagents • NGS reagents Contract Services • Antigens & Antibodies • Cell & Viral Banking • PCR/qPCR Assay Development

Global Presence MERIDIAN BIOSCIENCE, INC. Parent Company | Founded in 1977 | Nasdaq: VIVO Headquartered in Cincinnati, OH | 750+ Employees | Presence in 70+ Countries.

North America

EMEA

Asia Pacific

SYDNEY, AUSTRALIA Warehouse & Sales BEIJING, CHINA Wholly Owned Subsidiary

MEMPHIS, TN Viral Antigens Recombinant Proteins In Vitro Antibodies PCR Reagents BILLERICA, MA

LONDON, UK PCR Manufacturing & Sales PCR /qPCR Molecular Reagents LUCKENWALDE, GERMANY Large Scale Nucleotides PCR Enzymes Manufacturing PARIS, FRANCE EU Diagnostics Sales & Admin WATERLOO, BELGIUM EU Diagnostics Sales & Admin MILAN, ITALY EU Diagnostics Sales & Admin MODI’IN, ISRAEL BreathID ® Breath Test Systems

Magellan, Leadcare BOCA RATON, FL In Vivo Antibodies QUEBEC, CANADA GenePOC, Molecular Diagnostics

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Company Overview

• 3400+ products • High specificity & sensitivity • Antibody pairing • Large lot sizes

Antigens & Antibodies

Infectious disease

ToRCH • Toxo • Rubella • CMV

CHILDHOOD • Mumps • Rubeola • EBV • Coxsackie • Rotavirus • RSV • Parvo B19 • VZV RESPIRATORY • RSV

• HSV-1, 2 TROPICAL • Nipah • Dengue 1, 2, 3, 4

• Chikungunya • Mayaro Virus • Malaria • Chagas • Lassa Virus

GASTROINTESTINAL • Norovirus • Astrovirus • Adenovirus • Rotavirus •  Clostridium difficile

FOOD & WATER • Hepatitis A •  Campylobacter jejuni •  E. coli •  Legionella pneumophila •  Salmonella •  Shigella •  Bacillus anthracis •  Clostridium •  Listeria •  Streptococcus •  Staphylococcus •  Giardia lamblia •  Cryptosporidium

• Leishmaniasis • Leptospirosis • Newcastle disease • Yellow Fever • Zika • Lyme disease • Ebola STDs

• Influenza A,B • Parainfluenza •  Mycoplasma pneumoniae •  Chlamydia pneumoniae •  Legionella pneumophila •  Mycobacterium tuberculosis •  Streptococcus •  Staphylococcus • SARS Coronavirus • Adenovirus

•  Cryptosporidium •  Campylobacter •  E. coli •  Salmonella •  Giardia Iamblia •  H. pylori HEPATITIS

• HSV-1, 2 • HIV-1, 2 • HPV • Chlamydia • Neisseria • Syphilis

• HAV • HBV • HCV • HEV

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Cardiac & Metabolic Markers for Assay Development

Cancer

Cardiac

• PIVKA-II • CA125 • CA15-3 • CA19-9 • CA72-4 • CA50 • CA242 • CEA • Cyfra 21-1 • erbB-2/HER2 • AFP

• EGFR • HE4 • NSE • P53 • PMA

• Troponin I, T • Myoglobin • BNP • NT-proBNP • CRP • PCT • CK-MB • Apo A,B,E • NSE • FABP • SAH • Galectin-3

• Cystatin-C • D-Dimer • MPO • Fibrinogen • EGF • sCD40L • CD54 • Lp-PLA2 • PAPP-A • Vitamin D • Renin

• PAP • PSA

• PSMA • S-100 • B2M • Thyroglobulin

Immunoglobulins/Blockers

Hormones

• TRU Block™ & IgM Diluent • Animal IgGs – Bovine, Chicken, Goat, Mouse, Rabbit, Sheep • Human IgA, IgG, IgM, IgE

• LH, FSH, hCG, hGH • AMH • Cortisol, Estradiol • Insulin, C-peptide • Prolactin • Progesterone • PTH

• PAPP-A • TSH, T4, T3, ACTH • Thyroglobulin

• Kappa Light chain • Lambda Light chain • Goat Anti-Human IgG, IgM, IgA • Goat Anti-Mouse IgG

Drugs of Abuse • Amphetamine • Barbital • Benzodiazepine • Buprenorphine

• Fentanyl • Ketamine • K2 • MDMA (Ecstasy) • Methadone • Methamphetamine • Morphine

• Norketamine • Opium • Oxycodone • PCP • Phenobarbital • Propoxyphene • THC

• Cocaine • Cotinine • EDDP

Autoimmune

Allergens

• Jo-1 • PCNA • pANCA • cANCA • Sm Ag • dsDNA

• Histone • GMB • C1q • ScI-70 • B2-Gly-1

• Animal • Dust Mite • Mold • Pollen

• Cathepsin G • Calprotectin

• La (SS-B) • Ro (SS-A)

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Leading Cardiac Markers

Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP H86245M MAb to BNP ( Capture ), ELISA & WB H86507M MAb to BNP ( Detection ), ELISA & WB * Recognizes BNP. H86451M M Ab to NT-proBNP a.a. 61-76 (Capture) , ELISA & WB H86912M M Ab to NT-proBNP a.a. 1-12 (Detection) , ELISA & WB * Recognizes BNP, proBNP and NT-proBNP. K4A660R P Ab to BNP (Rabbit) ELISA, RIA, & IHC, lyophilized H01420M M Ab to NT-proBNP a.a. 61-76 ( Capture ), ELISA & WB H01419M M Ab to NT-proBNP a.a. 15-20 ( Detection ), ELISA & WB H01423M M Ab to NT-proBNP a.a. 15-20 ( Alternate Detection ), ELISA & WB * Detects NT-proBNP and proBNP. H01422M M Ab to NT-proBNP a.a. 5-12 (Capture) , ELISA & WB H01424M M Ab to NT-proBNP a.a. 22-36 (Detection) , ELISA & WB * Detects NT-proBNP regardless of glycosylation. Antibodies & Matched Pairs

Cystatin C H86023M H86218M H86013M

MAb (Capture) , ELISA MAb (Detection) , ELISA MAb (Alternate Detection)

D-dimer N01265M MAb, ELISA

N01273M MAb ( Capture ), ELISA & WB N01274M MAb ( Detection ), ELISA & WB N01270M MAb (Capture) , ELISA, LF & WB N01269H MAb (Detection) , ELISA, LF & WB * Highly specific for D-dimer and other cross linked fibrin degradation products containing D-dimer, but no reactivity with Fragment D or E. No cross-reaction was found with purified intact fibrinogen or fibrinogen degradation products. N86304M MAb (Capture) , ELISA & WB N86352M MAb (Detection) , ELISA & WB * Reacts with high molecular weight fibrin degradation products. Does not cross react with fibrinogen or D-monomer.

Fatty Acid Binding Protein (FABP) H01324M

MAb (Capture) , ELISA & LF MAb (Detection) , ELISA & LF

H01323M

Fibrinogen N86413M MAb, ELISA & WB

C-Reactive Protein (CRP) M86005M MAb (Capture) , ELISA M01239M MAb (Detection) , ELISA * Recognizes antigen in the presence and in the absence of Ca 2+ .

Galectin-3 K01361M

MAb, ELISA, WB & IHC

Creatine Kinase (CK) MAC01-288 MAb to CK-BB, ELISA H31795M

* Reacts with human and mouse Galectin-3. K01381M MAb, ELISA * Reacts with human and mouse Galectin-3.

M Ab to CK-MB (Capture/Detection) , ELISA & LF P Ab (Goat) to CK-MM (Capture/Detection) , ELISA & LF

Glycogen Phosphorylase Isoenzyme BB (GPBB) H86807M MAb, ELISA & WB

K31315G

* Abs can be used as capture or detection in a sandwich assay. MAC02-186 MAb to CK-MM, ELISA * 50% cross reactivity with CK-MB and < 1% cross reactivity with CK-BB. MAC02-512 MAb to CK-MM, ELISA * 52% cross reactivity with CK-MB, and no cross reactivity with CK-BB.

Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) H01390M MAb (Capture) , ELISA H01393M MAb (Detection) , ELISA H01389M MAb (Alternate Detection) , ELISA H01392M MAb (Capture) , ELISA H01393M MAb (Detection) , ELISA

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Myeloperoxidase (MPO) H87207M MAb, ELISA & IHC * Recognizes both native and recombinant human MPO.

Myoglobin (MYO) H86142M

Troponin I-Cardiac (cTnI) H86285M

MAb (Capture) , ELISA MAb (Detection) , ELISA

M Ab to a.a. 86-90 (Capture) , ELISA &WB

H86703M

H01347M M Ab to a.a. 24-40 (Detection) , ELISA & WB * Reacts equally with free cTnI and complexed cTnI, does not cross react with sTn. H01340M MAb to a.a. 41-49, ELISA H86241M MAb to a.a. 87-91, ELISA &WB H86596M MAb to a.a. 169-178, ELISA &WB K31015M MAb (Capture) , ELISA K31341G P Ab to a.a. 27-39 (Goat, Detection) , ELISA, WB & IHC K31342G P Ab to a.a. 69-86 (Goat, Alternate Detection) , ELISA & IHC * Cross reactivity with sTn is < 0.1% (ELISA).

H01328M H01327M

MAb (Capture) , ELISA & LF MAb (Detection), ELISA & LF

Procalcitonin (PCT) E86561M

MAb (Capture) , ELISA & WB MAb (Detection) , ELISA & WB

E86420M

* Specific to procalcitonin and calcitonin. E86412M

MAb (Capture) , ELISA MAb (Detection) , ELISA

E86813M

* Specific for procalcitonin and katacalcin.

Pregnancy Associated Plasma Protein A (PAPP-A) E86141M MAb (Capture) , ELISA & WB E86910M MAb (Detection) , ELISA & WB * Detects both heterotetrameric (found in placental blood) and homodimeric (found in atherosclerotic plaques) PAPP-A. E86114M MAb, ELISA & WB S-Adenosyl-Homocysteine (SAH) K01395C PAb (Chicken), ELISA * Affinity purified, treated with Adenine-9--D-Ribofuranoside to remove cross reaction to Adenosine.

Troponin T-Cardiac (cTnT) H86429M

M Ab to a.a. 60-70 (Capture) , ELISA &WB M Ab to a.a. 95-181 (Detection) , ELISA & WB

H86111M

* Does not cross react with sTn.

Vitamin D K01214M

MAb to Vitamin D (25 OH), ELISA, LF,

CLIA & ELISA * Pairs with antigens A01697B & A01698B. K01213M

MAb to Vitamin D (25 OH), ELISA & RIA

* Pairs with antigens A01697B & A01698B. K24124M-LQ MAb to 25-OH Vitamin D3, ELISA K24124M MAb to 25-OH Vitamin D3, ELISA K24123M MAb to 1,25 (OH) 2 Vitamin D3, ELISA *Recognizes Native Human 25 OH Vitamin D2 and 25 OH Vitamin D3.

Soluble CD40 Ligand (sCD40L) P86106M MAb, ELISA P42374M MAb, FC & IFA

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Leading Cardiac Markers

Human Antigens (for use as standards, controls & calibrators)

Brain Natriuretic Peptide (BNP), ProBNP + NT-ProBNP A24760H P roBNP, recombinant protein (E. coli) of N-terminal (a.a. 1-76, MW 8.39 kDa) A01261H N T-proBNP, recombinant protein (E. coli) of N-terminal (MW 8.589 kDa). Contains an additional methionine residue at the N-terminus (in comparison with native NT-proBNP) N86583H B NP and NT-proBNP Free Plasma, from

Fibrinogen A95150H F rom human plasma, MW 340kDa, isoelectric point of 5.1–6.3

Lp-PLA2 A01413H Recombinant (E.coli) ,> 95% pure (SDS-PAGE) Myeloperoxidase (MPO) A38104H F rom human neutrophils, > 96% pure (SDS-PAGE), approximate activity of 1,100 Units/mg Protein Myoglobin A38131H From human heart, 99% pure (SDS-PAGE) Pregnancy Associated Plasma Protein A (PAPP-A) A86864H F rom human retroplacental blood.

normal human plasma, used for preparation of any proBNP-related standards and calibrators

A97201H F rom human fluids and reactive with monospecific goat anti-CRP, 95% pure (SDS-PAGE)

Creatine Kinase (CK) VTI830

C K-BB, produced in cell culture (yeast), represents native full length protein and is enzymatically active C K-MB Isoenzyme Type I, produced in cell culture (yeast), enzymatically active CK-MB Isoenzyme Type II, produced in cell culture (yeast), represents native full length protein and is enzymatically active C K-MM Isoenzyme Type I, produced in cell culture (yeast), represents full length protein without the C-terminal lysine on both subunits and is enzymatically active C K-MM Isoenzyme Type III, produced in cell culture (yeast), represents full length protein and is enzymatically active

Heterotetrameric complex consisting of PAPP-A and pro-MBP subunits, > 85% pure (SDS-PAGE), lyophilized

VTI840

Procalcitonin (PCT) A01367H Recombinant protein (E. coli) representing a.a.

VTI810

3-116 which corresponds to the unprocessed human protein, contains a 6-His tag at N-terminal

VTI850

Troponin (cTnT & cTnI) A86111H I-T-C Complex, from human heart, formed by combining cardiac Troponin T, I and C in a molar ratio of 1:1:1 A86813H c TnT, from human heart, > 98% pure (SDS-PAGE) A38150H c TnI, from human heart, > 98% pure (SDS-PAGE), single band ~24 kDa Vitamin D A01694D  25-OH Vitamin D3 BSA Conjugate, ≥ 99% Pure (HPLC) A01698B  Vitamin D Biotin Conjugate, > 95.7% Pure (HPLC) A01697B Vitamin D BSA Conjugate A50674H  Vitamin D Binding Protein (Gc-Globulin), > 95% Pure (SDS-PAGE) A01409H 1,25 Dihydroxy Vitamin D3, > 99% Pure (HPLC)

VTI820

Cystatin-C A01259H From human urine, 95.5% pure A01435H F rom human urine, 95% pure (SDS-PAGE), major band at ~13 kDa, suitable for TIA applications D-Dimer A01408H From human plasma, > 90% pure A01463H From human plasma, > 95% pure (SDS-PAGE) Fatty Acid Binding Protein (FABP) A86865H From human heart, > 95% pure (SDS-PAGE)

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Product list

Adiponectin A protein hormone that modulates a number of metabolic processes including glucose regulation and fatty acid oxidation. Low levels of adiponectin are associated with the increased prevalence of obesity-linked cardiovascular disease, including ischemic heart disease and peripheral arterial disease. Antibody Pairs

Abbreviations 6-His – Polyhistidine-tag

Aff.Pur. – Affinity Purified, analyte-specific column Alk.Phos. – Alkaline Phosphatase conjugated product C. Eggs – Chicken Eggs CLIA – Chemiluminescence Immunoassay CVD – Cardiovascular Disease DB – Dot Blot DFA – Direct Immunofluorescence Assay FC – Flow Cytometry FITC – Fluorescein conjugated product GST – Glutathione S-transferase HRP – Horseradish peroxidase conjugated product IB – Immunoblot

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION H01263M H01262M H01286M H01285M H01289M H01262M H01290M H01263M H01296M H01297M H01297M H01296M

* Detects total Adiponectin * Detects total Adiponectin

* Detects low molecular weight form * Detects low molecular weight form

* Detects total Adiponectin * Detects total Adiponectin

Antigens A01260H

ICC – Immunocytochemistry IEP – Immunoelectrophoresis IFA – Immunofluorescence Assay

• Native antigen from pooled human plasma • Affinity purified, > 95% pure (SDS-PAGE)

IgG – Immunoglobin G IgM – Immunoglobin M IHC – Immunohistochemistry IP – Immunoprecipitation LF – Lateral Flow Lysate – Cells which have been lysed MAb – Monoclonal antibody Monospecific – Single band when tested by immunoelectrophoresis Neat – Whole, unpurified, undiluted antisera Neph – Product has been quality controlled by Nephelometry PAb – Polyclonal antibody Purified/IgG – Refer to the Certificate of Analysis regarding the extent of purification and the purification process used. RIA – Radioimmunoassay RID – Radial Immunodiffusion SDS-PAGE – Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis TIA – Product has been quality controlled by Turbidimetry WB – Western blot

Albumin

Hypoalbuminemia is a medical condition in which blood levels of albumin are abnormally low. It is a common condition in patients with heart failure and becomes more prevalent with increasing age and illness. It can be used as an independent predictor of incident heart failure in end-stage renal disease and elderly patients. Monoclonals • Recognizes human serum albumin in ELISA • Produced in vivo H86424M Antibody Pairs • Suitable for use in ELISA, WB and the quantitative detection of human serum albumin in serum and urine • Does not react with bovine serum albumin or egg white albumin • MAbs produced in vivo CAPTURE DETECTION H86157M H86910M H86157M H86611M

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Product list continued

Polyclonals CATALOG

Albumin continued

SOURCE FORMAT

APPLICATION

Polyclonals CATALOG

K45252G Goat K45252P Goat K01234G Goat K23600G Goat K04329G Goat K97110S Sheep

Aff.Pur.

EIA,WB EIA,WB

SOURCE FORMAT

APPLICATION

HRP

Monospecific IEP

L01242G Goat G5P01-0484 Goat K01263G Goat K04301G Goat K62110G Goat K90032P Sheep

Monospecific TIA,Neph,IEP

Neat

RID

Biotin

EIA

Monospecific IEP

Monospecific IEP Monospecific IEP Monospecific IEP

Aff.Pur.

EIA, WB

* K04329G & K97110S are specific for ApoA only (not ApoA I)

HRP

EIA

Apolipoprotein A-II (Apo A-II) Apo A-II is the second most abundant protein of HDL particles and plays an important role in reverse cholesterol transport and lipid metabolism. Apo A-II is mainly produced in the liver and modulates lipoprotein lipase and hepatic triglyceride lipase. The Apo A-II test can be used as an aid in assessing the risk of CVD. Polyclonals CATALOG SOURCE FORMAT APPLICATION K45890G Goat Neat EIA,WB K97211R Rabbit Aff.Pur. EIA,IB G5C21-766 Goat Aff.Pur. IEP K34001G Goat Aff.Pur. EIA,WB K45890S Sheep Neat EIA,WB K34004G Goat Biotin EIA,WB K74001P Goat HRP EIA,IB Antigens A50216H • Native antigen from human plasma HDL • > 95% pure (SDS-PAGE) Apolipoprotein B (Apo B) Apo B is the primary apolipoprotein in LDL particles and high levels of Apo B and occurs in the plasma in two main isoforms, Apo B48 and Apo B100. Apo B48 is a unique protein to chylomicrons from the small intestine and Apo B100 is found in lipoproteins originating from the liver. It is well established that Apo B100 levels are associated with coronary heart disease, and serve as a better predictor of LDL levels.

Antigens H8P01-767 • Native antigen from aphoresis plasma • Low B12 and low folate

Angiotensin

Apolipoprotein A-I (Apo A-I) Apo A-I is the major protein component of high density lipoprotein (HDL) in plasma and plays a role in lipid metabolism. Monoclonals • Does not cross-react with Apo A-II or Apo B • Suitable for use in RIA and ELISA • Produced in vivo system, which is a major target for drugs that lower blood pressure. Angiotensin I is converted to Angiotensin II through the enzyme angiotensin-converting enzyme (ACE). ACE inhibitors are widely used in the treatment of arterial hypertension and cardiovascular diseases. Monoclonals • Suitable for use in ELISA • Produced in vivo E01296M (Angiotensin I) * Does not cross-react with II and III E01297M (Angiotensin II) * Cross-reacts with I and III Antigens A01310S • Synthetic antigen (Asn-Arg-Val-Tyr-Val-His-Pro-Phe-OH) • > 98% pure (HPLC), lyophilized Angiotensin is a peptide hormone that causes vasoconstriction leading to high blood pressure. It is part of the renin-angiotensin

MAC20-001 MAC20-029 H45402M H45404M H45625M

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Antigens A50302H

Apolipoprotein B (Apo B) continued

• Native antigen from human plasma VLDL • 95% pure (SDS-PAGE)

Monoclonals • Suitable for use in ELISA and RIA • Produced in vivo

Apolipoprotein CIII

MAC23-031 MAC23-035 H45160M H45161M H45640M H45606M Polyclonals CATALOG

A protein that regulates both lipoprotein lipase and lecithin- cholesterol acyltransferase and may cause hypertriglyceridemia. Plasma levels of lipoprotein Apo CIII predict coronary heart disease and are associated with metabolic syndrome. Polyclonals CATALOG SOURCE FORMAT APPLICATION K74130G Goat Neat EIA,IB K74140G Goat Aff.Pur. EIA,IB K74140P Goat HRP EIA,IB K74170B Goat Biotin EIA,IB K97113R Rabbit Aff.Pur. EIA,IB Antigens A34129H • Native antigen from human plasma (VLDL) • 95% pure (SDS PAGE) Apolipoprotein E (Apo E) A major component of very low-density lipoproteins (VLDLs) and binds to a specific receptor on liver and peripheral cells. There are 3 major alleles: E3, E4, and E2. E3 is the normal form with normal funciton, the E2 variant has a reduced ability bind receptors and is associated with a high risk of CVD and the E4 variant is associated with increased levels of LDL- cholesterol and decreased levels of HDL-cholesterol, which is associated with a higher risk of developing coronary artery disease. Monoclonals

SOURCE FORMAT

APPLICATION

Apo B G5C23-766 Goat K01235G Goat K04323G Goat K62200G Goat Apo B–48/100 K45253G Goat K34003G Goat K34005G Goat

Aff.Pur.

N/A

Monospecific IEP Monospecific IEP

Monospecific EIA,IEP,TIA

Aff.Pur.

EIA,WB EIA,WB EIA,WB

Biotin

HRP

Antigens A50220H

• Native Apo B100 from human plasma LDL • > 95% pure (SDS-PAGE) and lyophilized • Native Apo B100 from human plasma LDL • Cross-reacts with anti-LDL

A34300H

Apolipoprotein AI/B (Apo AI/B) Calibrator

Antigens A62934H

• Reacts with VLDL of human plasma • Suitable for use in ELISA and IHC • Produced in vivo , lyophilized H61529M Polyclonals CATALOG

• Native antigen from human plasma • Apo AI: 313.0 mg/dL (lot dependent) • Apo B: 307.0 mg/dL (lot dependent)

Apolipoprotein CII (Apo CII)

SOURCE FORMAT APPLICATION

Apo CII acts as a co-factor for lipoprotein lipase, an enzyme that hydrolyses triglycerides in chylomicrons and VLDL. Can be used as an aid in assessing the risk of cardiovascular disease. Polyclonals CATALOG SOURCE FORMAT APPLICATION K74322G Goat Neat EIA,IB K97112R Rabbit Aff.Pur. EIA,IB

G5C27-766 Goat K34002G Goat K74190G Goat K62710G Goat K74180B Goat H01378G Goat

Aff.Pur.

N/A

HRP

EIA,WB EIA,WB

Aff.Pur.

Monospecific IEP,TIA

Biotin

EIA,WB

Purified

IEP

Antigens A50120H

• Native antigen from human plasma (VLDL) • > 95% pure (SDS-PAGE)

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Product list continued

Polyclonals CATALOG

BNP, ProBNP & NT-ProBNP BNP (B type natriuretic peptide) is a hormone and important biomarker with an established role in the diagnosis of congestive heart failure. Its utility has also been explored in myocardial ischemia and infarction, and in acute pulmonary embolism. When BNP is secreted it is attached to a 76–amino acid N-terminal fragment called NT-proBNP which is biologically inactive. Once released, BNP binds to and activates the atrial natriuretic factor (ANP) receptors. Monoclonals • Reacts with proBNP, N-terminal • Suitable for proBNP and NT-proBNP immunoassay H86705M a.a. 13-27 H86010M a.a. 28-45 *Also works in WB H86916M a.a. 46-60 *Also works in WB H86288M a.a. 61-76 Antibody Pairs • Suitable for use in ELISA and WB • Specific for Human BNP unless otherwise specified CAPTURE DETECTION H86051M H86245M H86051M H86262M

SOURCE FORMAT APPLICATION

K4A660R Rabbit K4A670R Rabbit K24410R Rabbit

Neat

EIA,IFA,IHC,RIA

Purified

EIA,IHC,RIA

Neat

RIA

Antigens R01614

• proBNP recombinant (E. coli) • Suitable for enzyme linked fluorescent assay • 95% pure (Silver Staining SDS-PAGE)

A24108H

• proBNP recombinant (E. coli) • > 95% pure (SDS-PAGE)

A01455H

• proBNP recombinant (E. coli) • Suitable for use in ELISA as a calibrator, standard or an immunogen for antibody development • Contains one additional Methionine residue at the N-Terminus (in comparison with native proBNP) • > 95% pure (Tricine SDS-PAGE)

A24810H

• proBNP, C-terminal (synthetic) • Represents amino acids 1-32 • > 95% pure (HPLC), lyophilized

H86245M H86507M H86245M H86573M H86051M H01365M H01365M H86507M H01365M H86573M H86245M H01255M H01365M H01366M H86051M H86915M

R01616

• NT-proBNP recombinant (E. coli) • > 95% pure (Silver Staining SDS-PAGE) • Suitable for enzyme linked fluorescent assay

A01261H

• NT-proBNP recombinant (E. coli) • Suitable for use in ELISA • > 95% pure (Tricine SDS-PAGE)

*Recognizes BNP & pro-BNP

*Recognizes BNP & pro-BNP

*Recognizes pro-BNP

A24607H

• proBNP, N-terminal recombinant (synthetic) • Represents a.a.1-76 • > 95% pure (HPLC), lyophilized • proBNP, N-terminal recombinant (E. coli) • Represents the N-terminal a.a.1-76 • > 95% pure (SDS-PAGE), lyophilized

H86451M

H86132M

*Recognizes proBNP & NT-proBNP

H86451M H86912M H86451M H86915M H86511M H86214M H01420M H01422M H01420M H01419M H01420M H01423M

*Recognizes proBNP & NT-proBNP

*Recognizes proBNP & NT-proBNP

A24760H

*Recognizes proBNP & NT-proBNP

*Detects NT-proBNP and proBNP

*Detects NT-proBNP and proBNP

*Detects NT-proBNP and proBNP

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11 | Cardiac & Metabolic Markers for Assay Development Cardiac & Metabolic Markers for A say Develop ent

Antigens A33123H

Carboxy Methyl Lysine (CML) A well-characterized glycoxidation product, formed from the oxidation of both carbohydrates and lipids, which accumulates in tissues with age. CML is a biomarker of general oxidative stress which is involved in the pathogenesis of atherosclerosis. Polyclonals CATALOG SOURCE FORMAT APPLICATION K97135G Goat Aff. Pur. EIA,WB

• Native Human Collagen Type III • Sourced from placental villi, > 90% pure (SDS-PAGE) • Suitable as a immunoassay standard and for antibody production

C-peptide & Proinsulin

C-peptide is a combination of amino acids that are a by-product of insulin and the level of this protein indicates how much insulin the pancreas is producing. Studies have shown an association between serum C-peptide levels and risk of cardiovascular-related disease and coronary artery-related mortality among adults without diabetes. Monoclonals • Reacts with human free C-peptide and Proinsulin • Suitable for use in ELISA and RIA • Produced in vivo

sCD40 Ligand (sCD40L)

A member of the TNF superfamily primarily expressed on activated T cells. Studies suggest that sCD40L may be at the heart of the atherosclerotic process, specifically playing a role in the inflammatory aspects of atherosclerotic lesion progression, thrombosis and restenosis. Monoclonals • Suitable for use in ELISA and WB • C an be used for detection of human sCD40L in serum P86210M

E01336M E86588M • Reacts with C-peptide • Suitable for use in ELISA and RIA E86710M MHP18-601 MHP18-6014 * Biotin conjugated

Collagen Type I & III

Increased myocardial collagen accumulation is present in almost every cardiac disease and plays an important role in the reduced heart function. N-terminal and C-terminal propeptides of Collagen Type I and III are the two major collagen types in the heart and reflect collagen synthesis and degradation. Their role as serum biomarkers for the diagnosis of cardiac fibrosis is being studied. Monoclonals • Specific for both native and heat denatured human Collagen Type I • Suitable for ELISA and IHC M1A340M Polyclonals CATALOG SOURCE FORMAT APPLICATION Collagen Type I T33131G Goat Aff.Pur. EIA,IB,ICC,IHC Collagen Type III T40233R Rabbit Purified EIA,IFA,IHC

C-Reactive Protein (CRP)

CRP is mainly used as a maker of inflammation. High- sensitivity CRP assays can be used for determining the risk of cardiovascular disease, heart attacks, and stroke. Antibody Pairs • Suitable for use in ELISA, high sensitivity • MAbs produced in vivo CAPTURE DETECTION M86005M M01239M * Recognizes CRP with or without Ca2+. M01243M M01239M * Recognizes CRP with or without Ca2+. M01331M M01330M

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Product list continued

Antigens VTI830

C-Reactive Protein (CRP) continued

• Recombinant (P. pastoris) • Full length protein with amino acid sequence identical to the native enzyme

Monoclonals • Recognizes CRP with or without the presence of Ca 2+ • Suitable for use in ELISA • Produced in vivo M01238M * Can also be used in IHC and WB

• > 95% pure (SDS-PAGE) • Suitable for use in ELISA H6C01-323 • Native antigen from human brain

M66112M M66113M M86007M M86842B

• Activity: 730 Units/mL at 37ºC (lot dependent)

* Can also be used in IHC

Creatine Kinase MB (CK-MB)

* Biotin conjugated

M86842M • Recognizes CRP • Suitable for use in ELISA (Unless otherwise specified) • Produced in vivo M01319B * Biotin conjugated

CK-MB is expressed in heart muscle and it is used in the diagnosis of acute myocardial infarction and possible reinfarction. The half- life of CK-MB in the circulation is relatively short (approximately 12 hours). CK-MB is relatively cardiac-specific, even healthy people may have low concentrations of this isoenzyme in their blood. Monoclonals • Suitable for use in ELISA and LF • Produced in vivo

M01319M M01295M

* No applications have been tested

Polyclonals CATALOG

H01298M H01299M Antibody Pairs • Suitable for use in ELISA and LF • MAbs produced in vivo CAPTURE DETECTION H31795M K31315G

SOURCE FORMAT APPLICATION

L01237G Goat L01238G Goat M01333C C. Egg

Purified Purified Aff. Pur.

IEP IEP N/A

Antigens A01430H

• Native antigen from human fluids • 98% pure (SDS-PAGE) • Native antigen from human fluids • 95% pure (SDS-PAGE, Non-Reduced) • Suitable for use in IEP and RID

* K31315G is a polyclonal Goat anti-CK-MM * K31315G is a polyclonal Goat anti-CK-MM

K31315G H31795M

A97201H

H01330M H01329M Antigens VTI840

A01250H

• Recombinant (E. coli) • Pentamer structure

• Recombinant (P. pastoris) , Isoenzyme Type I • Suitable for use in ELISA • Recombinant (P. pastoris) , Isoenzyme Type I • Suitable for use in ELISA

VTI810

Creatine Kinase BB (CK-BB)

CK-BB is found mostly in the brain and increases in response to brain injury, meningitis, abnormal cell growth, severe shock, stroke, hypothermia, or restricted blood flow to the bowel. It is used in creatine kinase test which determines what percentage of the total creatine kinase is due to the type produced by damaged heart muscles (CK-MB). Monoclonals • Produced in vivo MAC01-288

Creatine Kinase MM (CK-MM)

Found in your skeletal muscle and heart. CK-MM generally rises if you have muscle damage in your heart, brain, or skeleton after a crush injury, seizures, muscular dystrophy, muscle inflammation, or another skeletal muscle disorder. It is used in creatine kinase test which determines what percentage of the total creatine kinase is due to the type produced by damaged heart muscles (CK-MB).

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13 | Cardiac & Metabolic Markers for Assay Development ardiac etabolic arkers for say evelop ent

Creatine Kinase MM (CK-MM) continued

Cystatin C

Monoclonals • Suitable for use in ELISA • Produced in vivo

Traditionally used as a biomarker of kidney function, it has recently been studied for its role in predicting new onset or deteriorating CVD. In different cardiovascular conditions, Cystatin C is a sensitive marker for renal dysfunction, a predictor of the development of heart failure, and is independently associated with subsequent CVD and outcome. Antibody Pairs

• 52% cross-reactivity to CK-MB • 0% Cross-reactivity to CK-BB

MAC02-186 MAC02-512 Polyclonals CATALOG

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION H86023M H86013M Polyclonals CATALOG

SOURCE FORMAT

APPLICATION

G5C02-766 Goat H01310G Goat K31315G Goat

Aff.Pur. Purified Aff.Pur.

N/A N/A

EIA,LF

SOURCE FORMAT APPLICATION

* K31315G pairs with MAb to CK-MB H31795M Antigens VTI850

H01371C C. Egg

Aff.Pur.

N/A

Antigens A01259H

• Recombinant (P. pastoris) , Isoenzyme Type I • Full length protein without the C-terminal lysine on both subunits • Suitable for use in WB and ELISA • Recombinant (P. pastoris) , Isoenzyme Type I • Identical amino acid sequence to native protein • Suitable for use in ELISA • Native antigen from human skeletal muscle • > 95% pure (SDS-PAGE)

• Native antigen from human urine • 95.5% Pure

A01419H

• Native antigen from human urine

VTI820

D-Dimer

D-dimer is a marker of fibrin turnover and elevated levels are indicative of the presence of a clot and have been reported in deep vein thrombosis, pulmonary embolism, disseminated intravascular coagulation, acute aortic dissection, myocardial infarction, malignant diseases, obstetrical complications, third trimester of pregnancy, and surgery. It is a marker for the early diagnosis of acute coronary syndromes and can be used in addition to traditional assessment for myocardial infarction. Monoclonals • D oes not cross-react with fibrinogen and D-monomer (unless noted otherwise) • S uitable for use in ELISA and WB N86925M * Reacts with high molecular weight fibrin degradation products N86925B * Biotin conjugated

A31315H

A38925H

• Native antigen from human heart, lyophilized

CX3CR1

A receptor for fractalkine, a novel membrane-bound chemokine that is mainly expressed in endothelial cells in myocardial ischaemia and heart failure. When bound to CX3CR1, fractalkine acts as an adhesion molecule and can enable leucocyte adhesion. Under inflammatory conditions, this bound form can be cleaved to release a soluble chemokine implicated in a number of disease states, including atherosclerosis, glomerulonephritis, and cerebral ischaemia. Polyclonals CATALOG SOURCE FORMAT APPLICATION K88201R Rabbit Aff.Pur. IHC,WB * Extracellular Loop V28 K88093R Rabbit Aff.Pur. FC,IHC(p),WB * N-Terminal V28

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Product list continued

D-Dimer continued

Endothelin-1

Antibody Pairs • Suitable for use in ELISA and WB • Reactive with D-Dimer and other fribin degradation products • MAbs produced in vivo CAPTURE DETECTION N01270M N01269H * Does not cross-react Fragment D or E, also suitable for LF N01273M N01274M * Does not cross-react with Fibrinogen

Endothelin-1 is produced by the endothelium in response to Angiotensin II, inflammatory mediators, and vascular shear stress, this protein is responsible for vasoconstriction, activation of reactive oxygen species, and ventricular remodeling. Clinical trials have demonstrated the potential benefits of endothelin antagonists for patients with essential hypertension, pulmonary hypertension and heart failure. Monoclonals • Suitable for IRMA assays and IHC on frozen sections • Specific for Endothelin ET-1 • Produced in cell culture H54085M

N86304M N86925M N86304M N86043M Polyclonals CATALOG H01371C C. Egg

SOURCE FORMAT APPLICATION

Aff.Pur.

N/A

Epidermal Growth Factor (EGF)

Antigens A01408H

EGF is a growth factor with potential use as cardiac biomarker due to its role in the proliferation of cardiac stem cells. Research suggests that the human heart has an endogenous reserve of cardiac stem cells (CSCs) which can be activated by various growth factors to reconstitute injured myocardium. EGF has shown to significantly promote the proliferation, migration, and wound healing activities of these CSCs in comparison to the other growth factors. Antigens VTI880 • Recombinant (P. pastoris)

• Native antigen from human plasma • > 90% pure (SDS-PAGE and gel-scanning) • Native antigen from human plasma • Suitable for ELFA • 95% pure (SDS-PAGE) • Native antigen from human plasma • > 90% pure (SDS-PAGE and gel-scanning), lyophilized

A01463H

A86870H

• Suitable for use in WB • >95% pure (SDS-PAGE)

Endothelial Nitric Oxide Synthase (eNOS)

A42115H

• Recombinant (E. coli) • >98% pure (RP-HPLC and SDS-PAGE), lyphilized

Nitric oxide synthases are a family of enzymes catalyzing the production of nitric oxide (NO) from L-arginine. eNOS specifically generates NO in blood vessels which serves as a vasoprotective factor enabling endothelial cells to fight off vascular disease. In patients with cardiovascular risk factors such as hypertension, hypercholesterolemia, diabetes mellitus, or chronic smoking, the production of reactive oxygen species is stimulated in the vascular wall leading to eNOS dysfunction and subsequent CVD. Monoclonals • Specifically recognizes eNOS phosphorylated at serine 1177 • Suitable for EIA and WB • Produced in vivo Q67250M

Fatty Acid Binding Protein (FABP)

Heart type FABP is a small cytoplasmic protein (15 kDa) released from cardiac myocytes following an ischemic episode. It is an early biomarker for myocardial infarction, detected in the blood within one to three hours of acute coronary syndrome. Antibody Pairs

• Suitable for use in ELISA • MAbs produced in vivo CAPTURE H86505M H01314M H86101M H01314M H01315M H01324M H01323M H86101M

DETECTION

* Also works in LF assays

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15 | Cardiac & Metabolic Markers for Assay Development ardiac etabolic arkers for say evelop ent

Fatty Acid Binding Protein (FABP) continued

Galectin-3

Antigens A86865H

Galectin-3 is a galactoside-binding protein G that binds to and activates myofibroblasts in damaged heart tissue, leading to collagen synthesis and subsequently problematic fibrosis. It has been correlated with the prognosis in patients with coronary heart failure and the first Galectin-3 biomarker assay was approved by the FDA in 2010. Monoclonals K01361M *Suitable for use in WB,IHC,IP and ICC, produced in vivo K01381M *Suitable for use in FC, produced in vivo K01382M *Suitable for use in FC, produced in cell culture Antigens A01421H • Recombinant (E.coli) • 95% pure (SDS-PAGE)

• Native antigen from human heart • Suitable for use as a immunoassay standard, immunogen for antisera production, or a tracer for iodination • > 95% pure (10-20% gradient SDS-PAGE) • FABP3 Recombinant (E.coli) • Suitable for use in ELISA • 95% pure (silver staining SDS-PAGE)

R01617

Fibrinogen/ Fibrinopeptide A Fibrinogen is a plasma glycoprotein that is essential for blood blotting and has the ability to promote thromboses, or clots, by causing platelets to clump inside blood vessels. A number of studies have shown elevated fibrinogen levels to be a major risk factor for coronary heart disease. Antibody Pairs • Suitable for use in ELISA

Ghrelin

Clinical studies suggest a role for ghrelin in the treatment of CHF. Known cardiovascular effects of ghrelin include lowering of peripheral resistance either direct at the vascular level, and/ or by modulating sympathetic nervous activity. Gherlin also has potential as a marker of abnormal metabolism in cardiac failure. Monoclonals • Suitable for use in ELISA • Produced in vivo E86621M Glycogen Phosphorylase Isoenzyme BB (GPBB) GPBB is an isoenzyme of glycogen phosphorylase and a key enzyme of glycogenolysis. It has been suggested as an early marker of acute myocardial infarction and unstable angina. Monoclonals • Suitable for use in ELISA and WB • Produced in vivo H86807M

• For specific fibrinogen detection, pair a capture anti-fibrinopeptide A antibody with a detection anti-fibrinogen antibody • MAbs produced in vivo CAPTURE DETECTION N86710M N86413M * N86710M is specific for Fibrinopeptide A Polyclonals CATALOG

SOURCE FORMAT APPLICATION

K01394C C. Eggs

Aff.Pur.

N/A

Antigens A95150H

• Native antigen from human plasma • Isoelectric point: 5.1 - 6.3

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Hemoglobin A1c (HbA1c) As glucose circulates in the blood, some of it spontaneously binds to hemoglobin to form hemoglobin A1c (HbA1c). The development of specific diabetes complications correlates with glycated haemoglobin (HbA1c), the most accepted measure of chronic glycaemia. Monoclonals

Lectin-like Oxidized LDL Receptor-1 (sLOX) Soluble Form

A major endothelial receptor for oxidized low-density lipoprotein which is believed to play a role in atherosclerosis. Its expression is induced in pro-atherogenic settings including hypertension, hyperlipidemia, and diabetes, and it accumulates in therosclerotic lesions where it has been strongly linked to plaque rupture. Specifically sLOX-1 levels are elevated in acute coronary syndrome at an early stage, suggesting its usefulness as an early diagnostic marker of acute coronary syndrome. Monoclonals • Specific for human recombinant sLOX-1 (a.a. 58-273) • Suitable for use in ELISA and WB • Produced in vivo H86922M

• Suitable for use in sandwich ELISA • Does not cross-react with HbA10 • Produced in vivo H01331M

* Can also work in indirect ELISA (antigen capture)

H01388M

* Can also work in WB

Antibody Pairs • Suitable for use in ELISA • MAbs produced in vivo CAPTURE DETECTION H01291M H01292M H01367M H01369M H01367M H01368M

Leptin

* H01291M is an MAb to hemoglobin

A 16-kDa protein hormone that plays a key role in regulating energy intake and energy expenditure, including appetite/ hunger and metabolism. It has been proposed that leptin is an important link between obesity and the development of cardiovascular embolism. An association between serum leptin concentrations and various cardiovascular risks, including stroke, chronic heart failure and myocardial infarction has been

* H01367M is an MAb to hemoglobin

* H01367M is an MAb to hemoglobin

Antigens A01273H

• Native antigen from human erythrocytes • > 96% HbAlc (HPLC analysis using a Mono S Column)

observed. Antigens A42327H

Interleukin 8 (IL-8) IL-8 is a neutrophil chemokine produced by macrophages, lymphocytes, epithelial cells and endothelial cells, and promotes neutrophil chemotaxis. Studies in patients with CAD have demonstrated that IL-8 may predict cardiovascular events independent of the other cytokines and high sensitivity CRP. Antigens A42280H • Recombinant (E. coli), a.a. 1-72 • > 98% pure (HPLC, FPLC and reducing and non-reducing SDS-PAGE) A42208H • Recombinant (E. coli) , a.a. 1-77 • > 98% pure (HPLP and SDS-PAGE)

• Recombinant (E. coli) • > 95% pure (RP-HPLC and SDS-PAGE)

Lipoproteins HDL, LDL, VLDL Receptor

Lipoprotein diagnostics, such as LDL cholesterol and HDL cholesterol, help to diagnose dyslipidemia which is an important risk factor for cardiovascular disease and type II diabetes. Monoclonals • MAb to LDL Receptor • R ecognizes an epitope in the region of repeat #1 of the ligand binding region • Suitable for WB, FC, IFA H44070M • MAb to LDL Receptor-Related Protein (LRP) • Will not block ligand binding • Suitable for use in ELISA, WB, IHC, and FC H63650M

17

17 | Cardiac & Metabolic Markers for Assay Development Cardiac & Metabolic Markers for A say Develop ent

Lipoprotein-Associated Phospholipase A2 (Lp-PLA2) Lp-PLA2 is a protein produced by inflammatory cells that circulates mainly with LDL and is responsible for hydrolyzing oxidized phospholipids in LDL. It is highly upregulated in atherosclerotic plaques and is thought to be directly involved in the development of atherosclerosis and plaque rupture. Monoclonals

Lipoproteins HDL, LDL, VLDL Receptor continued

Antigens A94005H

• Native HDL from human plasma • > 95% of total lipoprotein content by electrophoresis

A95132H

• Native HDL from human normolipidemic plasma • Devoid of VLDL, IDL and LDL • Ultracentrifugation at density 1.063–1.210 • Native HDL 2 (HDL2) Subfraction from human normolipidemic plasma • Devoid of VLDL, IDL, LDL, and HDL3 • Ultracentrifugation density at 1.063 - 1.125 • Native HDL 3 (HDL3) Subfraction from human normolipidemic plasma • Devoid of VLDL, IDL, LDL, and HDL2 • Ultracentrifugation density at 1.120–1.21g/mL • Reconstituted HDL from human plasma • A complex of human Apolipoprotein A-I and 1-palmitoyl-2-oleoyl phosphatidylcholine prepared at a molar ratio of 1 to 100 by the cholate removal method • Native LDL from human plasma • > 95% pure (total lipoprotein content by electrophoresis) • Suitable for use in IEP • Native VLDL from human plasma • Devoid of IDL, LDL, and HDL • Purification by ultracentrifugation at density 1.006 • Native VLDL from human plasma • > 95% of total lipoprotein content (SDS-PAGE) • Suitable for use in ELISA and IEP

• Suitable for use in ELISA • Produced in cell culture H01389M Antibody Pairs • Suitable for use in ELISA • Produced in cell culture CAPTURE DETECTION H01390M H01393M H01390M H01389M H01392M H01393M Polyclonals CATALOG

A95322H

A95332H

A34275H

SOURCE FORMAT APPLICATION

H01332S Sheep H01333S Sheep

Purified Aff.Pur.

EIA,IHC,WB EIA,IHC,WB

A95124H

Antigens A01413H

• Recombinant (E. coli) • > 95% pure (SDS-PAGE) • Contains a His-tag

A34013H

A62924H

• Lp(a) Calibrator • Sourced from defibrinated human plasma • Suitable for use in Turbidimetry

A50261H

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