Glycerol-Free Taq Hot-Start DNA Polymerase
Meridian’s Glycerol-Free Taq DNA polymerase is a high-concentration (50 U/ µ L) enzyme optimized to deliver robust amplification without glycerol. It is supplied as a separate 10x glycerol‑free Taq DNA Polymerase, glycerol‑free Taq antibody, and glycerol‑free enzyme dilution buffer, making it compatible with lyophilization for assay manufacturers developing room-temperature stable assays. The high enzyme concentration allows for bulk production of PCR master mixes and the glycerol-free format is favorable when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial. > High concentration (50 U/ µ L) enzyme supplied as a separate Taq, hot-start antibody, and enzyme dilution buffer, all glycerol-free, for the ultimate flexibility in assay development > Ideal for efficient large-scale production of PCR master mixes and applications using automated pipetting robots or where accurate pipetting of small volumes is critical > Compatible with lyophilization for developing ambient-temperature stable assays
Figure 3. Glyercol-Free Hot Start Polymerase: Efficient and sensitive amplification used as wet mix or after lyophilization
Figure 4. Enzyme maintains stability after freeze-thaw cycles
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F/T Cycles
Glycerol is a cryoprotectant and is normally part of the storage buffer, where it serves to protect Taq polymerase during freezing conditions. To test the resistance of Glycerol‑Free Taq Hot‑Start to freezing and thawing, a freeze‑thaw test was performed (10 and 15 cycles of freeze/thawing) and compared to fresh product (0 cycles of freeze/thawing). The results illustrate that Glycerol‑Free Taq Hot‑Start offers the same protection to freeze/thawing as a polymerase that contains glycerol.
A 10-fold serial dilution of human DNA (10 3 copies down to 10 copies) using Glycerol-Free Taq HS 50U/ μ L as a wet mix ( black ) or lyophilized mix ( blue ) to illustrate that lyophilization does not have an effect on the quality of the Glycerol-Free Taq HS.
PRODUCT
CAT NO.
VOLUME
REACTIONS
0.02 mL
1,000 Rxns
Glycerol-Free Taq HS, 50 U/ µ L
MDX011
5 mL
25,000 Rxns
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