55C MMLV-RT. Robust thermostable reverse transcriptase ideal for ambient temperature stable molecular assays.
55C MMLV-RT
GC-rich Regions
Robust thermostable reverse transcriptase ideal for ambient temperature stable molecular assays.
R everse transcriptase activity up to 60°C
Ideal for RNA with high secondary structure such as viral genomes
S ensitive detection of low copy number RNA targets
Helices
Ideal for developing fast, highly reproducible RT-qPCR assays
Challenges with RNA Secondary Structures
Product Information
PRODUCT
CAT NO.
VOLUME
REACTIONS
50 µL
10,000 Units
55C MMLV-RT Thermostable Moloney Murine Leukemia Virus (concentration 200 U/µL)
200 µL
40,000 Units
MDX117
1 mL
200,000 Units
10 mL
2,000,000 Units
Traditional Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) is not thermostable and can only maintain its enzymatic activity at relatively low temperatures (up to 50°C). However for cDNA synthesis, a higher reaction temperature is desirable as it reduces RNA secondary structures which can inhibit reverse transcription and it minimizes nonspecific primer binding. Meridian has developed 55C MMLV-RT a new reverse transcriptase that has higher thermal stability and reduced RNase H activity. The enzyme can be used to synthesize first-strand cDNA at temperatures up to 60°C, which improves the cDNA yield from difficult RNA targets that require higher temperature to denature strong secondary structures.
Comparison of thermostability of 55C MMLV-RT vs other RTases
Price comparison (March 2023)
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0.04
0.03
0.02
0.01
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TEMPERATURE (ºC)
PACK SIZE
MMLV-RT | SuperScript III (Thermo) | 55C MMLV-RT
SuperScript III (Thermo) | 55C MMLV-RT
Performance of Meridian’s 55C MMLV-RT (teal) , standard MMLV-RT (grey) and SuperScript III thermostable reverse transcriptase (Thermo, black) after pre-incubation at 40°C to 70°C for 10 mins, in a multiplex one-step RT-qPCR assay. ∆ ct values were calculated against the ct value produced by the same enzyme stored at -20 °C. The data illustrates the increased thermal stability of 55C MMLV-RT when compared to other MMLV-RT enzymes and its ability to efficiently synthesize cDNA at temperatures up to 60°C.
55C MMLV-RT can help you save on your raw material costs which is critical in today’s challenging business environment.
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Product Performance Data
Higher Enzyme Efficiency and Sensitivity
55C MMLV-RT is designed for greater efficiency of the reverse transcription reaction, improving cDNA yield and enabling a lower limit of detection (LOD) with higher sensitivity in one-step RT-qPCR assays.
B2MG
Rps18L
55C MMLV-RT | SuperScript III (Thermo)
The sensitivity of 55C MMLV-RT (teal) was compared to SuperScript III (Thermo, black) in a multiplex one-step RT-qPCR assay using a 10-fold serial dilution of mammalian total RNA. The results demonstrate that 55C MMLV-RT has higher performance with better sensitivity and end-fluorescence.
Ideal for High-Throughput, Multiplex One-Step RT-qPCR Assays
55C MMLV-RT exhibits robust performance in one-step RT-qPCR assays detecting RNA viruses with strong secondary structures.
Flu A
MERS-CoV
RSV
55C MMLV-RT
55C MMLV-RT was tested in a triplex one-step RT-qPCR assay on respiratory RNA virus targets (Influenza A, MERS-CoV and RSV). The results demonstrate the sensitivity and reproducibility of 55C MMLV-RT even in challenging conditions such as multiplex RT-qPCR reactions where targets may differ greatly in abundance.
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience. com www.MeridianLifeScience.com
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