Product Performance Data
Higher Enzyme Efficiency and Sensitivity
55C MMLV-RT is designed for greater efficiency of the reverse transcription reaction, improving cDNA yield and enabling a lower limit of detection (LOD) with higher sensitivity in one-step RT-qPCR assays.
55C MMLV-RT | SuperScript III (Thermo)
The sensitivity of 55C MMLV-RT (teal) was compared to SuperScript III (Thermo, black) in a multiplex one-step RT-qPCR assay using a 10-fold serial dilution of mammalian total RNA. The results demonstrate that 55C MMLV-RT has higher performance with better sensitivity and end-fluorescence.
Ideal for High-Throughput, Multiplex One-Step RT-qPCR Assays
55C MMLV-RT exhibits robust performance in one-step RT-qPCR assays detecting RNA viruses with strong secondary structures.
55C MMLV-RT was tested in a triplex one-step RT-qPCR assay on respiratory RNA virus targets (Influenza A, MERS-CoV and RSV). The results demonstrate the sensitivity and reproducibility of 55C MMLV-RT even in challenging conditions such as multiplex RT-qPCR reactions where targets may differ greatly in abundance.
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