Commercial Assays & Recommended Products
Toxoplasma IgM
Detection System
Recommended Products
Assay Type
Solid Phase
Antigen
Mouse anti-human IgM, F(ab’)2 to Toxo & Rec. p30 antigen
Z01235M, C01523M and R01573 Z01235M, C01523M and 8200 Z01235M, C01523M and 8200 Z01235M, C01523M and 8200 W01259G, C01523M and 8200
Abbott ARCHITECT
CMIA IgM-capture sandwich method
Paramagnetic microparticles
Direct, CL
Abbott AxSYM
MEIA IgM-capture sandwich method
Microparticles Mouse anti-human IgM, MAb to Toxo p30 & native lysate Indirect, MUB
Siemens IMMULITE
CLIA IgM-capture sandwich method
Paramagnetic particles
Mouse anti-human IgM, MAb to Toxo & p30 antigen
Direct, CL
DiaSorin LIAISON
CLIA IgM-capture sandwich method
Paramagnetic particles
Mouse anti-human IgM, MAb to Toxo & native antigen Goat anti-human IgM, MAb to Toxo p30 & native antigen (RH Sabin strain)
Indirect, CL
BioMérieux VIDAS
ELFA IgM-capture sandwich method
Solid phase receptacles
Direct, MUB
Toxoplasma IgG
Detection System
Recommended Antigens
Assay Type
Solid Phase
Antigen
Abbott ARCHITECT ARCHITECT Avidity
Paramagnetic microparticles Paramagnetic microparticles
Rec. antigens to 30 and p35 (GRA8), anti-human IgG Rec. antigens to 30 and p35 (GRA8), anti-human IgG
CMIA
Indirect, CL R01573 and R01581
CMIA 2 assays with and without blocking buffer to dissociate low-avidity antibodies
Indirect, CL R01573 and R01581
Siemens AVIDA Centaur
Paramagnetic particles
CLIA
Native p30 antigen
Direct, CL
8200
Siemens IMMULITE 2000
Paramagnetic particles Paramagnetic microparticles Paramagnetic microparticles
Partially purified antigen, mouse anti-human IgG
CLIA
Indirect, CL 8200
DiaSorin LIAISON DiaSorin LIAISON Avidity
Native antigen, anti-human IgG Native antigen, anti-human IgG
CLIA
Indirect, CL 8200
CLIA 2 assays with and without a 6M urea elution step to dissociate low-avidity antibodies
Indirect, CL 8200
BioMérieux VIDAS BioMérieux VIDAS Avidity
Solid phase receptacles Solid phase receptacles
ELFA
Native antigen
Direct, MUB 8200
ELFA 2 assays (reference and test) with and without a disassociation wash step to remove low-avidity
Native antigen (RH Sabin strain), anti-human IgG
Direct, CL
8200
ELFA: Enzyme-linked fluorescence assay CLIA: Chemiluminescent Immunoassay CMIA: Chemiluminescent Microparticle Immunoassay MUB: Methyl-umbellifery CL: Chemiluminescence MEIA: Microparticle Enzyme Immunoassay ECLIA: Electrochemiluminescence Immunoassay
6 | ToRCH REAGENTS - IgG and IgM Assay Development
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