ToRCH IgG/IgM Assay Development Guide

Commercial Assays & Recommended Products continued

CMV IgG

Detection System

Recommended Antigens

Assay Type

Solid Phase

Antigen

Abbott ARCHITECT i2000SR Abbott ARCHITECT Avidity

Paramagnetic microparticles Viral lysate

CMIA

Indirect, CL 7600 or 7511

CMIA, two assays with and without liquid CMV antigen to neutralize high-avidity CMV antibodies

Paramagnetic microparticles Viral lysate, anti-human IgG Indirect, CL 7600 or 7511

Abbott AxSYM MEIA

Microparticles Viral lysate

Indirect, MUB 7600 or 7511

Siemens IMMULITE 2000 Roche ELECSYS Roche ELECSYS Avidity BioMérieux VIDAS

Paramagnetic particles Paramagnetic microparticles Paramagnetic microparticles

CLIA

Purified Antigen

Indirect, CL 7600 or 7511

ECLIA, one step double sandwich method

Rec. antigens CMV pp150, pp52, pp28, pp38 Rec. antigens CMV pp150, pp52, pp28, pp38

Direct, CL R01561 or R01563

ECLIA, two assays with and without chaotropic conditions to dissociate low-avidity antibodies

Direct, CL R01561 or R01563

ELFA two step sandwich method Solid phase receptacles

Viral lysate, anti-human IgG Direct, MUB 7600 or 7511

ELFA two assays with and without 6 M urea to dissociate low-avidity antibodies

BioMérieux VIDAS Avidity

Solid phase receptacles

Viral lysate, anti-human IgG Direct, MUB 7600 or 7511

HSV-1 & HSV-2 IgG/IgM

Detection System

Recommended Antigens

Assay Type

Solid Phase

Antigen

DiaSorin LIAISON HSV-1 Type Specific DiaSorin LIAISON HSV-2 Type Specific

Magnetic particles

Rec. HSV-1 gG 1 and mouse anti-human IgG

CLIA, two steps

Indirect, CL VTI520

Magnetic particles

Rec. HSV-2 gG 1 and mouse anti-human IgG

CLIA, two steps

Indirect, CL VTI530

Focus PLEXUS HerpesSelect® 1 and 2 IgG Biorad BIOPLEX 2200 HSV-1 & HSV-2 IgG

Rec. HSV-1 gG 1, HSV-2 gG 1 and goat anti-human IgG Rec. gG1 and synthetic peptide p8C:BSA derived from gG2 sequence (patented) and mouse anti-human IgG and mouse anti-human FXIII

Indirect, Fluorescent

VTI520, VTI530 and L15406G

EIA multiplex flow three steps

Beads

Carboxy-coated dyed beads

Direct, Fluorescent

EIA multiplex flow three steps

VTI520 and VTI530

Roche ELECSYS HSV-1 IgG

ECLIA, two step antigen sandwich

Paramagnetic microparticles Rec. HSV-1, HSV-1 viral lysate Direct, CL VTI520 and 7305

Siemens Immunlite 2000 Herpes 1/2 IgG

HSV-1 (strain MacIntyre), HSV-2 (strain G) viral lysate and anti-human IgG

Paramagnetic particles

CLIA

Indirect, CL 7305 and 7705

Life Science Division 5171 Wilfong Road | Memphis, TN 38134 +1 901-382-8716 www.MeridianLifeScience.com

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