ToRCH IgG/IgM Assay Development Guide

Rubella IgM

Detection System

Recommended Products

Assay Type

Solid Phase

Antigen

Abbott ARCHITECT i2000SR Roche ELECSYS Siemens IMMULITE 2000 Siemens AVIDA Centaur XP

Paramagnetic microparticles

Viral lysate (strain HPV77) and mouse anti-human IgM MAb Indirect, CL 6076/6123

CMIA

Recombinant rubella like particles and rubella-specific antibodies

CLIA IgM-capture sandwich method Magnetic beads

Indirect, CL EV9525 or EV9526

CLIA IgM-capture sandwich method Polystyrene beads

Viral lysate (strain HPV77)

Indirect, CL 6076/6123

Viral lysate and rubella specific antibody

CLIA IgM-capture sandwich method Paramagnetic particles

Indirect, CL 6076/6123 and EV9525 or EV9526

Rubella IgG

Detection System

Recommended Products

Assay Type

Solid Phase

Antigen

Abbott ARCHITECT i200SR

Partially purified viral lysate (strain HPV77), mouse anti-human IgG MAb

Paramagnetic microparticles

CMIA

Indirect, CL 6075

Abbott AxSYM

MEIA

Microparticles Purified viral lysate and anti-human IgG

Indirect, MUB 6075

Siemens AVIDA Centaur XP Siemens IMMULITE 2000 Beckman ACCESS 2 Roche ELECSYS BioMérieux VIDAS Ortho VITROS Eci

Purified viral lysate (strain HPV77) and mouse anti- human IgG FC

Paramagnetic particles

CLIA moderate complexity

Indirect, CL 6075

Paramagnetic particles Paramagnetic particles

CLIA moderate complexity

Viral lysate

Indirect, CL 6075

EIA

Viral lysate

Indirect, CL 6075

Recombinant E1 antigen, rubella like particles and rubella specific antibodies Viral lysate (strain MR383) and anti-human IgG Viral lysate and mouse-anti- human IgG

CLIA, double antigen sandwich with an IgG-capture method ELFA, sandwich immunoassay method

Magnetic beads

Indirect, CL EV9525 or EV9526

Solid phase receptacles

Indirect, MUB 6075

Indirect, Luminescence 6075

CLIA high complexity

Plastic wells

CMV IgM

Detection System

Recommended Products

Assay Type

Solid Phase

Antigen

Viral lysate (strain AD169), rec. antigen pp150 and pp52, and anti-human IgM Rec. antigen CMV pp150, pp52, pp65, pp38, and anti-human IgM Purified antigen (strain AD169), Goat anti-human IgG, goat anti-human IgM

Abbott ARCHITECT

Paramagnetic microparticles

CMIA

Indirect, CL R01561, R01563 and W01259G

R01561, R01563, R01567 and W01259G 7600 or 7511 and W01259G and L15406G R01561, R01563 and Z01235M 7600 or 7511 and Z01235M

Abbott AxSYM

MEIA

Microparticles

Indirect, MUB

Siemens IMMULITE 2000 Roche ELECSYS BioMérieux VIDAS

CLIA 3 step assay, IgM-capture sandwich method ECLIA IgM-capture sandwich method

Paramagnetic particles Paramagnetic microparticles

Direct, CL

Rec. antigens CMV pp150 and pp52, and anti-human IgM Direct, CL Viral lysate (strain AD169), mouse anti-human IgM Indirect, MUB

ELFA IgM-capture sandwich method Solid phase receptacles

www.MeridianLifeScience.com | 7

Powered by