Ambient temperature stable qPCR Master Mixes

Sensitive Detection in Multiplex Assays in the Presence of Stool Inhibitors

Lyo-Ready™ Direct DNA qPCR Stool (MDX142)

Escherichia coli O157

Salmonella typhimurium

Campylobacter jejuni

Lyo-Ready TM Direct DNA qPCR Stool | KAPA Probe Force | Ultraplex TM

E. coli O157 (Shiga-like/Verotoxin 2 gene), Salmonella typhimurium (16S-23S rRNA gene internal transcribed spacer) and Campylobacter jejuni (16S-23S rRNA gene internal transcribed spacer) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Stool (MDX142, red ) and universal mixes, KAPA Probe Force (Roche, green ) and Ultraplex TM (QuantaBio, grey ) in the presence of 6.6 mg/mL human stool. The results illustrate that Lyo-Ready™ Direct DNA qPCR Stool is not affected by high concentrations of human stool, unlike the other mixes, allowing higher multiplexing capacity and greater reproducibility.

Ideal for Liquid Format or Lyophilized Assays – No Impact on Reaction Efficiency

A) Lyo-Ready TM Direct DNA qPCR Stool (MDX142)

B) Lyo-Ready TM Direct RNA/DNA qPCR Stool (MDX143)

10,000 copies 1,000 copies

10,000 copies 1,000 copies

100 copies 10 copies

100 copies 10 copies

lyophilized | liquid

Liquid ( blue ) and lyophilized ( red ) formats of A) Lyo-Ready TM Direct DNA qPCR Stool (MDX142) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100, and 10 copies respectively) in presence of 20% artificial stool and B) Lyo-Ready TM Direct RNA/DNA qPCR Stool (MDX143) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively) in presence of 5% artificial stool. The results illustrate that the lyophilized mixes retain the ability to efficiently amplify to the same level as the liquid mixes with the same level of sensitivity and reproducibility.

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