Superior reaction efficiency using crude urine samples in multiplex reactions
Lyo-Ready™ Direct DNA qPCR Urine (MDX152)
MDX152 | QuantaBio ToughMix® | Roche KAPA | ThermoFisher TaqPath TM
Four sexually transmitted pathogens ( M. genitalium, T. pallidum (Syphilis), C. trachomatis and N. gonorrhoeae) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready TM Direct DNA qPCR Urine (MDX152) in a lyophilized format ( red ) and compared to QuantaBio ToughMix® PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath TM ProAmp TM Multiplex Master Mix ( black ) in a liquid format. The results demonstrate that Lyo-Ready TM Direct DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
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