More confidence when low-copy targets matter. Residual E. coli DNA associated with recombinant enzymes produced in bacterial systems can create background signal and increase false-positive risk. Meridian non-bacterial (Non-Bac) qPCR solutions use a eukaryotic expression system to support sensitive detection of low-copy bacterial targets.
Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
Background bacterial DNA does not have to be an accepted limitation.
More confidence when low-copy targets matter Residual E. coli DNA associated with recombinant enzymes produced in bacterial systems can create background signal and increase false-positive risk. Meridian non-bacterial (Non-Bac) qPCR solutions use a eukaryotic expression system to support sensitive detection of low-copy bacterial targets. From development to commercialization Start with the format that suits your development workflow today, while retaining a path to stabilization later. Choose MDX252 for custom formulation or MDX362 for a complete Lyo-Ready qPCR mix.
Choose the format that matches your workflow
MDX252 Custom formulation Design control for custom PCR/qPCR development.
MDX362 Ready-to-use Complete 4x qPCR mix for ready-to-use simplicity.
MDX252
Lyo-Ready Non-Bac DNA qPCR Mix • Complete 4x mix: Hot-start qPCR mix for sensitive low-copy multiplex detection. • Non-Bac format: Designed to help reduce bacterial DNA background. • Liquid or lyophilized: Supports ambient-stable assay formats. MDX362 Glycerol-Free Non-Bac Taq HS Polymerase (HC) • High concentration: 50 U/ µ L hot-start Taq polymerase. • Custom formulation: Glycerol-free format for PCR and qPCR mix development. • Dry-format compatible: Supports lyophilized assay development with appropriate excipients.
Both support wet-format development today with a path to lyophilization later.
ORDERING INFORMATION Product CAT. No
Size
1 mL (200 reactions) 5 mL (1,000 reactions) 50 mL (10,000 reactions) 20 µ L (1,000 units) 500 µ L (25,000 units) 5 mL (250,000 units)
Lyo-Ready Non-Bac DNA qPCR Mix
MDX362
Glycerol-Free Non-Bac Taq HS Polymerase (HC)
MDX252
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data
Designed for Sensitive Bacterial DNA Detection The completed 4x hot-start mix containing optimized buffer chemistry, PCR enhancers, dNTPs (including dUTP), MgCl 2 and Glycerol-Free Non-Bac HS Taq polymerase, supports low-copy and multiplex qPCR testing. It can be used as a liquid mix or lyophilized for ambient-stable assay formats.
10,000 copies 1,000 copies 100 copies 10 copies 1 copy
Lyo-Ready Non-Bac DNA qPCR Mix in liquid (blue) and lyophilized (red) format s were compared for their ability to amplify a 10-fold serial dilution of a bacterial DNA target (10,000, 1,000, 100, 10 and 1 copies respectively). The results illustrate that the lyophilized mix maintains the same high application efficiency as the liquid mix, with high sensitivity and reproducibility. NTCs are shown in (black) .
No Detectable Bacterial DNA Contamination
Using a pan-bacterial 16S assay, MDX362 showed high sensitivity and reproducibility while the no-template control remained without detectable amplification. Lyo-Ready Non-Bac DNA qPCR Mix was used to amplify a 10-fold serial dilution of bacterial DNA target (10,000, 1,000, 100, 10, and 1 copies, respectively) in liquid (blue) format using a pan-bacterial 16S assay. No detectable amplification was observed in the no-template control (black) . The Lyo-Ready Non-Bac DNA qPCR Mix demonstrates high
10,000 copies 1,000 copies 100 copies 10 copies 1 copy
sensitivity and reproducibility, supporting reliable detection of low-copy bacterial DNA targets with no detectable bacterial background amplification under the conditions tested.
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX362 Performance Data continued
High Multiplexing Capability
P. aeruginosa
M. tuberculosis
E. coli
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
Three bacterial DNA targets were amplified using Lyo-Ready Non-Bac DNA qPCR Mix in liquid (blue) format, under standard qPCR cycling conditions. The results demonstrate the high sensitivity of Lyo-Ready Non-Bac DNA qPCR Mix, enabling reliable amplification of bacterial targets down to single-copy levels in multiplex reactions. NTCs are shown in (black) .
Lyophilized Assay Stability Lyo-Ready Non-Bac DNA qPCR Mix was lyophilized, and the stability of the dried assays (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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Non-Bac DNA qPCR Solutions Cleaner background for sensitive bacterial DNA detection
MDX252 Performance Data
High Multiplexing Capability 16S (Entero)
Mip
ToxA
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
1,000 copies 100 copies 10 copies 1 copy
The activity of the Glycerol-Free Non-Bac HS Taq Polymerase (HC) was tested in a triplex qPCR assay targeting marker genes from three bacteria (16S rRNA gene from Enterococcus , Mip gene from Legionella pneumophila and ToxA gene from Pseudomonas aeruginosa ), using a 10-fold serial dilution of the bacterial genomic DNA. The result shows that Glycerol-Free Non-Bac HS Taq Polymerase (HC) can successfully detect multiple targets down to 1 copy of DNA in multiplex assays.
Lyophilized Assay Stability Glycerol-Free Non-Bac HS Taq Polymerase (HC) was lyophilized in a lyo-ready DNA qPCR mix and the stability of the dried assay (red) was tested after accelerated stability against fresh liquid mix (blue) in a triplex qPCR assay. The lyophilized mix retained performance comparable to the liquid mix, supporting projected stability of more than 12 months at ambient temperature.
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