Lyo-Ready ™ & Air-Dryable ™ qPCR and RT-qPCR Master Mixes
Currently, qPCR/RT-qPCR is regarded as the ‘gold standard’ for the quantitative analysis of nucleic acids and Meridian’s Lyo-Ready TM and Air-Dryable TM qPCR and RT-qPCR master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. Lyo-Ready TM and Air-Dryable TM qPCR and RT-qPCR master mixes are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As ready-to-use mixes, they require no further optimization besides the addition of primers and probes. Within the Lyo-Ready TM and Air-Dryable TM range, Meridian offers universal inhibitor-tolerant qPCR and RT-qPCR formulations as well as Specimen-specific TM master mixes designed for crude blood, saliva, urine or stool samples. The universal mixes are highly resistant to various qPCR inhibitors and can be used across a range of patient sample types. The Specimen-specific TM range is more specialized and designed for inhibitor-rich samples or direct detection from a crude lysate, avoiding the need for DNA or RNA extraction and purification. These mixes are ultra-sensitive and are capable of detecting DNA down to a single copy and RNA down to 50 copies.
Product Features
• Available in universal or specimen-specific formulations for blood, saliva, urine or stool and suitable for direct detection assays using crude samples • High efficiency and sensitivity in multiplex reactions • Just add primers and probes, no other optimization required • Stable shelf-life for up to 24 months • Reduces cost and complexity of shipping and storage
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Specimen-specific ™ qPCR & RT-qPCR Mixes
Blood-Specific Mixes
Blood
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer (liquid biopsy),
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Blood Mix, 4x
MDX092
Air-Dryable Direct RNA/ DNA qPCR Blood Mix, 4x cardiovascular disease, and metabolic disorders including diabetes. However, there are several PCR inhibitors such as immunoglobulin G, hemoglobin, lactoferrin and leukocyte DNA that are present in blood which impact a reaction’s efficiency. In addition, anticoagulants used to stabilize blood samples (e.g., EDTA, citrate or heparin) contain a number of inhibitors which cause interference in a PCR reaction. Over the past decade, many diagnostic assays that use blood as a specimen have improved in terms of turnaround time, sensitivity and specificity as traditional techniques and biomarkers have been replaced by newer, faster and more sensitive methods. The traditional approaches to overcoming inhibition however have relied on removing inhibitors through DNA or RNA extraction prior to testing, but these methods are not 100% effective, cause sample loss, and reduce reproducibility. Lyo-Ready Direct DNA qPCR Blood Mixes Lyo-Ready Direct RNA/DNA qPCR Blood Mixes
MDX121
MDX122
MDX123
Meridian’s Air-Dryable TM and Lyo-Ready TM direct blood mixes are unique by enabling direct amplification of target DNA or RNA from high concentrations of crudely processed blood, serum or plasma samples. They have been designed to efficiently amplify in the presence of inhibitors found in blood – no further optimization is required. Furthermore, these mixes can be used in a wet format or lyophilized to create ambient-temperature stable assays.
High tolerance to whole blood stabilized with anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)
A) Air-Dryable™ Direct DNA qPCR Blood (MDX092)
B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121)
QuantaBio
TaqPath™ 1-Step
KAPA Probe Force
TaqPath™
K2-EDTA | Sodium heparin | Sodium citrate
Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. 0% 2%, 5% and 20% human whole blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) and sodium citrate ( grey ) was tested with (A) Air-Dryable TM Direct DNA qPCR Blood (MDX092) against mixes from KAPA Probe Force (Roche) and TaqPath™ (Thermo) and (B) Air-Dryable TM Direct RNA/DNA qPCR Blood (MDX121) against mixes from Ultraplex™ (QuantaBio) and TaqPath™ 1-Step (Thermo). The results demonstrate that the reaction efficiencies of the Air-Dryable TM Direct Blood mixes are higher both in the presence of anticoagulants and higher concentrations of blood than of other suppliers’ mixes.
Significantly greater tolerance to inhibitors found in whole blood and anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)
A) Lyo-Ready™ Direct DNA qPCR Blood (MDX122)
B) Lyo-Ready™ Direct RNA/DNA qPCR Blood (MDX123)
45
45
40
40
35
35
30
30
25
25
10%
2%
5%
10
2%
5%
2%
5%
10%
0%
2%
5%
0%
2%
5%
0%
2%
5%10%
%
10%
10%
®
TaqPath™
TaqPath™
MDX122
KAPA Probe Force (Roche)
MDX123
UltraPlex (Quantabio)
(ThermoFisher)
(ThermoFisher)
EDTA | Heparin | Citrate
Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. In this experiment, 0%, 2%, 5% and 10% whole human blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) or sodium citrate ( grey ) was tested with (A) Lyo-Ready TM Direct DNA qPCR Blood (MDX122) against KAPA Probe Force (Roche) and TaqPath™ (Thermo) and (B) Lyo-Ready™ Direct RNA/DNA qPCR Blood (MDX123) against UltraPlex™ 1-Step ToughMix® (QuantaBio) and TaqPath™ 1-Step Multiplex Master Mix (Thermo). The results demonstrate that the reaction efficiencies of the Lyo-Ready TM Direct Blood mixes are higher in the presence of inhibitors found in anticoagulants and high concentrations of blood.
High multiplexing capacity enables the detection of multiple analytes from crude, inhibitor- rich plasma, serum and whole blood samples
Lyo-Ready™ Direct DNA qPCR Blood (MDX122) S. aureus
P. falciparum
Epstein-Bar virus
Lyo-Ready TM Direct DNA qPCR Blood | KAPA Probe Force |TaqPath TM ProAMP TM Mix
Three diagnostic DNA targets ( Staphylococcus aureus , Plasmodium falciparum and Epstein-Bar virus) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Blood (MDX122, red ), KAPA Probe Force ( green ) or TaqPath™ ProAmp TM Mix ( black ) in the presence of 10% EDTA plasma or 10% serum. The results illustrate that Lyo-Ready™ Direct DNA qPCR Blood has higher multiplexing capacity and performance than KAPA Probe Force and TaqPath™ ProAmp™ Mix.
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Specimen-specific ™ qPCR & RT-qPCR Mixes
Saliva-Specific Mixes Over the last decade, saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection/logistics and similar to serum, saliva contains hormones, antibodies, growth factors, enzymes, and microbes that can be used as biomarkers for disease detection. However, there have been challenges adopting saliva for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors in saliva. Cumbersome sample preparation steps have been required to homogenize the specimen and adequately purify the DNA/RNA for molecular quantification. Meridian’s new Air-Dryable TM and Lyo-Ready TM direct saliva mixes are unique in that they have been designed to specifically overcome the inhibitors found in saliva or sputum samples. As a result, they are capable of highly sensitive amplification using crudely processed samples or extracted DNA or RNA in multiplex detection assays.
Saliva
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Saliva Mixes
MDX130
Air-Dryable Direct RNA/ DNA qPCR Saliva Mixes
MDX131
Lyo-Ready Direct DNA qPCR Saliva Mixes
MDX132
Lyo-Ready Direct RNA/DNA qPCR Saliva Mixes
MDX133
Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or UniversalTransport Media (up to 35% UTM)
Air-Dryable TM Direct DNA qPCR Saliva (MDX130)
Mycoplasma pneumoniae
ADV
CMV
0% human saliva | 10% human saliva | 20% human saliva | 60% human saliva
Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( black ), 10% ( red ), 20% ( blue ) and 60% ( grey ) homogenized human saliva. The results illustrate that the performance of the air-dried, Air-Dryable TM Direct DNA qPCR Saliva is not affected by increasing concentrations of human saliva.
Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or UniversalTransport Media (up to 35% UTM)
Air-Dryable TM Direct RNA/DNA qPCR Saliva (MDX131)
Influenza A
MERS-CoV
RSV
Air-dried | TaqPath TM (Thermo) | Ultraplex TM (QuantaBio)
Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 35% Universal Transport Media (UTM) with artificial sputum swab. The results illustrate that a higher performance was achieved with Air-Dryable TM Direct RNA/DNA qPCR Saliva (MDX131, red ) compared to the inhibitor-tolerant RT-qPCR mixes TaqPath TM (Thermo, black ) or Ultraplex TM (QuantaBio, grey ).
Efficient amplification using the mix in a wet or dried down format
Lyo-Ready TM Direct DNA qPCR Saliva (MDX132)
Lyo-Ready TM Direct DNA qPCR Saliva (MDX132) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100 and 10 copies respectively), in presence of 20% artificial sputum in both liquid ( blue ) and lyophilized ( red ) formats. The results illustrate that the lyophilized mix retains the ability to efficiently amplified to the same level as the liquid mix with the same level of sensitivity and reproducibility.
10,000 copies 1,000 copies 100 copies 10 copies
Lyophilized | Liquid
Lyo-Ready TM Direct RNA/DNA qPCR Saliva (MDX133)
Lyo-Ready TM Direct DNA/RNA qPCR Saliva (MDX133) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively), in the presence of 5% artificial sputum in both liquid ( blue ) and lyophilized ( red ) formats. The results illustrate that the lyophilized mix retains the ability to efficiently amplify to the same level as the liquid mix with the same level of sensitivity and reproducibility.
10,000 copies 1,000 copies 100 copies 10 copies
Lyophilized | Liquid
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Specimen-specific ™ qPCR & RT-qPCR Mixes
Stool-specific Mixes
Stool
PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed serious challenges to developing assays that can directly amplify DNA or RNA. In addition, due to the high complexity and heterogeneity of fecal matter, sample preparation has traditionally been required to remove possible interfering substances such as food debris, microorganisms, desquamated epithelial cells and mucus from the specimen. Air-Dryable ™ and Lyo-Ready ™ Stool mixes are designed for direct amplification from stool samples, and require only minimal sample processing. The mixes contain an optimized blend of additives to negate inhibitor effects while maintaining the quality and integrity of the patient sample. As the need for fast, non-invasive testing for gastrointestinal conditions increases, short turn-around times, higher sensitivity and a long shelf-life become important distinguishing features.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Stool Mixes
MDX140
Air-Dryable Direct RNA/ DNA qPCR Stool Mixes
MDX141
Lyo-Ready Direct DNA qPCR Stool Mixes
MDX142
Lyo-Ready Direct RNA/DNA qPCR Stool Mixes
MDX143
Sensitive detection in multiplex assays in the presence of stool inhibitors
Air-Dryable TM Direct RNA/DNA qPCR Stool (MDX141)
Adenovirus
Rotavirus
Campylobacter jejuni
Norovirus
Air-dried | TaqPath TM (Thermo) | Ultraplex TM (QuantaBio)
Two RNA targets (Rotavirus and Norovirus) and two DNA targets (Adenovirus and Campylobacter jejuni ) were amplified in a quadruplex reaction using Air-Dryable TM Direct RNA/DNA qPCR Stool (MDX141, red ) or mixes from TaqPath TM (Thermo, black ) or Ultraplex TM (QuantaBio, grey ) in the presence of 1.5 mg/mL bile salts. The results illustrate that Air-Dryable TM Direct RNA/DNA qPCR Stool is less affected by increased concentrations of bile, unlike the other mixes.
Sensitive Detection in Multiplex Assays in the Presence of Stool Inhibitors
Lyo-Ready™ Direct DNA qPCR Stool (MDX142)
Escherichia coli O157
Salmonella typhimurium
Campylobacter jejuni
Lyo-Ready TM Direct DNA qPCR Stool | KAPA Probe Force | Ultraplex TM
E. coli O157 (Shiga-like/Verotoxin 2 gene), Salmonella typhimurium (16S-23S rRNA gene internal transcribed spacer) and Campylobacter jejuni (16S-23S rRNA gene internal transcribed spacer) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Stool (MDX142, red ) and universal mixes, KAPA Probe Force (Roche, green ) and Ultraplex TM (QuantaBio, grey ) in the presence of 6.6 mg/mL human stool. The results illustrate that Lyo-Ready™ Direct DNA qPCR Stool is not affected by high concentrations of human stool, unlike the other mixes, allowing higher multiplexing capacity and greater reproducibility.
Ideal for Liquid Format or Lyophilized Assays – No Impact on Reaction Efficiency
A) Lyo-Ready TM Direct DNA qPCR Stool (MDX142)
B) Lyo-Ready TM Direct RNA/DNA qPCR Stool (MDX143)
10,000 copies 1,000 copies
10,000 copies 1,000 copies
100 copies 10 copies
100 copies 10 copies
lyophilized | liquid
Liquid ( blue ) and lyophilized ( red ) formats of A) Lyo-Ready TM Direct DNA qPCR Stool (MDX142) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100, and 10 copies respectively) in presence of 20% artificial stool and B) Lyo-Ready TM Direct RNA/DNA qPCR Stool (MDX143) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively) in presence of 5% artificial stool. The results illustrate that the lyophilized mixes retain the ability to efficiently amplify to the same level as the liquid mixes with the same level of sensitivity and reproducibility.
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Specimen-specific ™ qPCR & RT-qPCR Mixes
Urine-Specific Mixes
Urine
Urine is an ideal clinical specimen because it is excreted in large quantities, non-invasive, and can be self-sampled. Currently, urine specimens are used in the diagnosis and management of infectious diseases (including STDs), hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. However, urine contains substances such as urea and nucleases that can damage DNA or inhibit a PCR reaction. Air-Dryable ™ and Lyo-Ready ™ urine mixes are inhibitor-
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Urine Mixes
MDX150
Air-Dryable Direct RNA/ DNA qPCR Urine Mixes
MDX151
Lyo-Ready Direct DNA qPCR Urine
MDX152
tolerant and designed for very fast, highly sensitive amplification of DNA and RNA directly from high
Lyo-Ready Direct RNA/DNA qPCR Urine
MDX153
concentrations of urine and are sensitive enough to detect arboviruses, such as Chikungunya virus. Patient urine samples can be used directly on the dried assay, and do not require nucleic acid purification.
Enables highly sensitive detection of cancer markers in urine samples
Air-DryableTM Direct RNA/DNA qPCR Urine (MDX151)
CDC2
IGFBP5
MDK
MDK
Air-dried | Ultraplex TM (QuantaBio)
Three tumor-related mRNA markers (CDC2 kinase, IGFBP5 and MDK) were amplified from total RNA (from a bladder cancer patient) in a triplex reaction using air-dried Air-Dryable TM Direct RNA/DNA qPCR Urine (MDX151, red ) or Ultraplex TM (QuantaBio, grey ) in 10% human urine. The results illustrate that Air-Dryable TM Direct RNA/DNA qPCR Urine is able to detect cancer related RNA markers from urine faster and with higher sensitivity compared to the other mix.
Superior reaction efficiency using crude urine samples in multiplex reactions
Lyo-Ready™ Direct DNA qPCR Urine (MDX152)
Mycoplasma genitalium
Treponema pallidum
Chlamydia trachomatis
Neisseria gonorrheae
MDX152 | QuantaBio ToughMix® | Roche KAPA | ThermoFisher TaqPath TM
Four sexually transmitted pathogens ( M. genitalium, T. pallidum (Syphilis), C. trachomatis and N. gonorrhoeae) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready TM Direct DNA qPCR Urine (MDX152) in a lyophilized format ( red ) and compared to QuantaBio ToughMix® PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath TM ProAmp TM Multiplex Master Mix ( black ) in a liquid format. The results demonstrate that Lyo-Ready TM Direct DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
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Specimen-specific ™ qPCR & RT-qPCR Mixes
Urine-Specific Mixes (continued)
Urine
Superior reaction efficiency using crude urine samples in multiplex reactions (continued)
Lyo-Ready™ Direct RNA/DNA qPCR Urine (MDX153)
Dengue
Zika
Chikungunya
Malaria
MDX153 | ThermoFisher TaqPath TM | QuantaBio ToughMix® | BioRad Reliance 1-Step
Three viral RNA (Dengue, Zika and Chikungunya) and one DNA (Malaria) mosquito-borne infectious disease targets were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready TM Direct RNA/DNA qPCR Urine (MDX153) in a lyophilized format ( red ) and compared to ThermoFisher TaqPath TM 1-Step Multiplex Master Mix ( black ), QuantaBio Ultraplex TM 1-Step ToughMix® ( grey ) and BioRad Reliance 1-Step Multiplex RT-qPCR Supermix ( pink ) in liquid formats. The results illustrate that dry Lyo-Ready TM Direct RNA/DNA qPCR Urine has higher multiplexing capacity and reproducibility than the liquid competitor mixes.
Universal Lyo-Ready TM and Air-Dryable TM qPCR and RT-qPCR Mixes
Meridian’s Lyo-Ready™ and Air-Dryable™ qPCR and RT-qPCR master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol- free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient- temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various qPCR inhibitors and can be used across a wide range of patient sample types.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 500 Rxns 100 mL 10,000 Rxns 8 mL 1,000 Rxns 100 mL 12,500 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns
MDX082
Air-Dryable qPCR Mix, 4x
Air-Dryable RT-qPCR Mix, 4x
MDX095
MDX021
Lyo-Ready qPCR Mix
MDX023
Lyo-Ready qPCR Mix, 2.6x
Lyo-Ready 1-Step RT-qPCR Mix
MDX024
Lyo-Ready 1-Step RT-qPCR Virus Mix
MDX062
Full enzyme activity following air-drying
Air-Dryable™ RT-qPCR Mix (MDX095)
Activity of Air-Dryable™ 1-Step RT-qPCR Mix (MDX095) in both wet and air-dried formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mixup to the assay limit of detection.
Wet | Air-dried
Retention of reverse transcriptase activity following lyophilization
Air-Dryable™ RT-qPCR Mix (Air-Dryable™ RT-qPCR Mix (MDX095))
y-actin
GAPDH
B2mg
Primers and probes were added to Lyo-Ready™ 1-Step RT-qPCR Mix (MDX024), dried down and immediately rehydrated ( purple ) and tested against a freshly prepared wet mix ( orange ), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.
Lyophilized | Wet Mix
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Product List
• Air-Dryable ™ Direct DNA & DNA/RNA qPCR Blood (MDX092 & MDX121) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Blood (MDX122 & MDX123)) Blood
Air-Dryable mixes are a new alternative to lyophilization. Create ambient temperature stable assays using only an oven. What is Air-Dryable?
• Air-Dryable ™ Direct DNA & DNA/RNA qPCR Saiva (MDX130 & MDX131) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Saliva (MDX132 & MDX133) Saliva
• Air-Dryable ™ Direct DNA & DNA/RNA qPCR Stool (MDX140 & MDX141) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Stool (MDX142 & MDX143) Stool
• Air-Dryable ™ Direct DNA & DNA/RNA qPCR Urine (MDX150 & MDX151) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Urine (MDX152 & MDX153) Urine
• A ir-Dryable ™ qPCR Mix (MDX082) • A ir-Dryable ™ RT-qPCR Mix (MDX095) • L yo-Ready ™ qPCR Mix (MDX021) • L yo-Ready ™ qPCR Mix, 2.6X (MDX023) • L yo-Ready ™ 1-Step RT-qPCR Mix (MDX024) • L yo-Ready ™ 1-Step RT-qPCR Virus Mix ( MDX062) Universal
Learn more
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
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