Meridian is a primary manufacturer of specialized high-quality molecular reagents and offers solutions to a wide range of industries to diagnose and treat diseases, discover new therapeutics or develop tests for environmental, food and cosmetic safety.
Specimen-specific ™ and Universal qPCR & LAMP Master Mixes for Ultra-Sensitive Detection Compatible with lyophilization and air-drying to create ambient-temperature stable assays
Meridian’s Specimen-specific™ qPCR/RT-qPCR and LAMP /RT-LAMP master mixes are designed for ultra-sensitive amplification from crude blood, saliva, urine stool and plant material. They are 4x concentrated, glycerol-free and highly inhibitor-tolerant making them ideal for direct detection assays using crude lysate. Their ultra-sensitive detection enables amplification of DNA from a single copy or RNA from less than 50 copies. The mixes also contain a specialized blend of excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays. As ready-to- use mixes, they require no further optimization besides the addition of primers and probes. Molecular diagnostic tests are progressively moving towards lyophilized and air-dried formats. There are several advantages for this, including room-temperature shipping and storage, extended shelf-life, and increased flexibility in sample volume. Meridian’s Lyo-Ready™ and Air-dryable™ technologies simplify the development of ambient-temperature stable, multiplex molecular assays, allowing customers to control their entire manufacturing workflow, speed up the time spent on assay development, and significantly reduce the cost per test.
Product Features
• Ready-to-use, glycerol-free PCR and LAMP master mixes formulated with a specialized blend of excipients for lyophilization or air-drying. • Available in universal or Specimen-specific ™ formulations for blood, saliva, urine or stool that are suitable for direct detection assays from crude samples. • Specimen-specific ™ mixes are highly inhbitor-tolerant and enable ultra-sensitive detection down to single copies of DNA. • Mixes can be used as a liquid or lyophilized/air-dried to create ambient-temperature stable assays.
Blood Saliva Stool
Urine Universal
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qPCR and RT-qPCR Mixes
Currently, qPCR/RT-qPCR is regarded as the ‘gold standard’ for the quantitative analysis of nucleic acids with diverse applications such as gene expression analysis for molecular diagnostics, detection of genetically modified organisms in food, and cancer phenotyping. Meridian’s Lyo-Ready™ and Air-Dryable™ qPCR and RT-qPCR master mixes are the market-leading solution delivering fast cycling times, superior sensitivity, and high inhibitor tolerance for all assay types.
Specimen-specific™ Mixes
Blood-Specific Mixes
Blood
Blood is one of the most common specimens used for laboratory diagnostic testing and it is useful for evaluating the function of vital organs and for diagnosing diseases such as bacterial and viral infections, cancer (liquid biopsy), cardiovascular disease, and metabolic disorders including diabetes. However, there are several PCR inhibitors such as immunoglobulin G, hemoglobin, lactoferrin and leukocyte DNA that are present in blood which impact a reaction’s efficiency. In addition, anticoagulants used to stabilize blood samples (e.g., EDTA, citrate or heparin) contain a number of inhibitors which cause interference in a PCR reaction.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Blood Mix, 4x
MDX092
Air-Dryable Direct RNA/ DNA qPCR Blood Mix, 4x
MDX121
Lyo-Ready Direct DNA qPCR Blood Mixes
MDX122
Lyo-Ready Direct RNA/DNA qPCR Blood Mixes
MDX123
Meridian’s Air-Dryable™ and Lyo-Ready™ direct blood mixes are unique by enabling direct amplification of target DNA or RNA from crudely processed blood, serum or plasma samples. They have been designed to efficiently amplify in the presence of inhibitors found in blood – no further optimization is required.
High reaction efficiency on plasma, serum and whole blood samples containing anticoagulants
Air-Dryable™ Direct DNA qPCR Blood (MDX092)
Plasma
Serum
Air-dried | KAPA Probe Force | TaqPath™ ProAmp™
Plasmid DNA containing Epstein-Barr virus was spiked into 10% serum or 10% K2-EDTA plasma and amplified in a triplex reaction using air-dried MDX092 format ( red ) and kits from KAPA Probe Force ( green ) andTaqPath™ ProAmp™ ( black ).The results illustrate higher end florescence and better sensitivity with MDX092 than with mixes from KAPA andThermo.
High tolerance to whole blood stabilized with anticoagulants (K2-EDTA, sodium heparin, and sodium citrate)
A) Air-Dryable™ Direct DNA qPCR Blood (MDX092)
B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121)
KAPA Probe Force
TaqPath™
QuantaBio
TaqPath™ 1-Step
K2-EDTA | Sodium heparin | Sodium citrate
Anticoagulants and high concentrations of whole blood are known to inhibit qPCR efficiencies. 0% 2%, 5% and 20% human whole blood in the presence of K2-EDTA ( orange ), sodium heparin ( green ) and sodium citrate ( grey ) was tested with (A) Air-Dryable™ Direct DNA qPCR Blood (MDX092) against mixes from KAPA Probe Force (Roche) and TaqPath™ (Thermo) and (B) Air-Dryable™ Direct RNA/DNA qPCR Blood (MDX121) against mixes from UltraPlex™ (QuantaBio) and TaqPath™ 1-Step (Thermo). The results demonstrate that the reaction efficiencies of the Air-Dryable™ Direct Blood mixes are higher both in the presence of anticoagulants and high concentrations of blood than of other suppliers’ mixes. High multiplexing capacity enables the detection of multiple analytes from crude, inhibitor-rich plasma, serum and whole blood samples
Lyo-Ready™ Direct DNA qPCR Blood (MDX122)
S. aureus
P. falciparum
Epstein-Bar virus
Lyo-Ready™ Direct DNA qPCR Blood | KAPA Probe Force | TaqTath™ ProAmp™ Mix
Three diagnostic DNA targets ( Staphylococcus aureus , Plasmodium falciparum and Epstein-Bar virus) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Blood (MDX122, red ), KAPA Probe Force ( green ) or TaqPath™ ProAmp™ Mix ( black ) in the presence of 10% EDTA plasma or 10% serum. The results illustrate that Lyo-Ready™ Direct DNA qPCR Blood has higher multiplexing capacity and performance than KAPA Probe Force and TaqPath™ ProAmp™ Mix.
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qPCR and RT-qPCR Mixes
Saliva-specific Mixes
Saliva
Saliva has been extensively studied as a diagnostic specimen due to its non-invasive and simple sample collection/logistics. Similar to serum, saliva contains hormones, antibodies, growth factors, enzymes, and microbes that can be used as biomarkers for disease detection. However, there have been challenges adopting saliva for molecular diagnostics due to the low concentration of analytes and high concentration of PCR inhibitors. Meridian’s new Air-Dryable™ and Lyo-Ready™ direct saliva mixes are unique in that they have been designed to specifically overcome the inhibitors found in saliva or sputum samples. As a result, they are capable of highly sensitive amplification using crudely processed samples or extracted DNA or RNA in multiplex detection assays.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Saliva Mixes
MDX130
Air-Dryable Direct RNA/ DNA qPCR Saliva Mixes
MDX131
Lyo-Ready Direct DNA qPCR Saliva Mixes
MDX132
Lyo-Ready Direct RNA/DNA qPCR Saliva Mixes
MDX133
Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or UniversalTransport Media (up to 35% UTM)
Air-Dryable™ Direct DNA qPCR Saliva (MDX130)
Mycoplasma pneumoniae
ADV
CMV
0% human saliva | 10% human saliva | 20% human saliva | 60% human saliva
Mycoplasma pneumoniae genomic DNA, adenovirus (ADV) and cytomegalovirus (CMV) were amplified in a triplex qPCR assay in the presence of 0% ( black ), 10% ( red ), 20% ( blue ) and 60% ( grey ) homogenized human saliva. The results illustrate that the performance of the air-dried, Air-Dryable™ Direct DNA qPCR Saliva (MDX130) is not affected by increasing concentrations of human saliva.
Sensitive detection in multiplex assays using saliva samples (up to 60% human saliva) or UniversalTransport Media (up to 35% UTM)
Air-Dryable™ Direct RNA/DNA qPCR Saliva (MDX131)
Influenza A
MERS-CoV
RSV
Air-dried | TaqPath ™ (Thermo) | UltraPlex™ (QuantaBio)
Three respiratory pathogens, Influenza A, Middle East Respiratory syndrome coronavirus (MERS-CoV) and Respiratory Syncytial Virus (RSV) were amplified in a triplex qPCR assay in the presence of 35% Universal Transport Media (UTM) with artificial sputum swab. The results illustrate that a higher performance was achieved with Air-Dryable™ Direct RNA/DNA qPCR Saliva (MDX131, red ) compared to the inhibitor-tolerant RT-qPCR mixes TaqPath™ (Thermo, black) or UltraPlex™ (QuantaBio, grey ).
Efficient amplification using the mix in a wet or dried down format
Lyo-Ready™ Direct DNA qPCR Saliva (MDX132)
Lyo-Ready™ Direct DNA qPCR Saliva (MDX132) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100 and 10 copies respectively),in presence of 20% artificial sputum in both liquid ( blue ) and lyophilized ( red ) formats. The results illustrate that the lyophilized mix retains the ability to efficiently amplified to the same level as the liquid mix with the same level of sensitivity and reproducibility.
10,000 copies 1,000 copies 100 copies 10 copies
Lyophilized | Liquid
Lyo-Ready™ Direct RNA/DNA qPCR Saliva (MDX133)
Lyo-Ready™ Direct DNA/RNA qPCR Saliva (MDX133) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively), in the presence of 5% artificial sputum in both liquid ( blue ) and lyophilized ( red ) formats. The results illustrate that the lyophilized mix retains the ability to efficiently amplify to the same level as the liquid mix with the same level of sensitivity and reproducibility.
10,000 copies 1,000 copies 100 copies 10 copies
Lyophilized | Liquid
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qPCR and RT-qPCR Mixes
Stool-specific Mixes
Stool
PCR inhibitors found in stool specimens, such as bile salts, polysaccharides, hematin and catabolic substances, have posed serious challenges to developing assays that can directly amplify DNA or RNA. In addition, due to the high complexity and heterogeneity of fecal matter, sample preparation has traditionally been required to remove possible interfering substances such as food debris, microorganisms, desquamated epithelial cells and mucus from the specimen. Air-Dryable ™ and Lyo-Ready ™ Direct Stool mixes are designed for direct amplification from stool samples, and require only minimal sample processing. The mixes contain an optimized blend of additives to negate inhibitor effects while maintaining the quality and integrity of the patient sample. As the need for fast, non-invasive testing for gastrointestinal conditions increases, short turn-around times, higher sensitivity and a long shelf-life become important distinguishing features.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Stool Mixes
MDX140
Air-Dryable Direct RNA/ DNA qPCR Stool Mixes
MDX141
Lyo-Ready Direct DNA qPCR Stool Mixes
MDX142
Lyo-Ready Direct RNA/DNA qPCR Stool Mixes
MDX143
Sensitive detection in multiplex assays in the presence of stool inhibitors
Air-Dryable ™ Direct RNA/DNA qPCR Stool (MDX141)
Adenovirus
Rotavirus
Campylobacter jejuni
Norovirus
Air-dried | TaqPath ™ (Thermo) | UltraPlex™ (QuantaBio)
Two RNA targets (Rotavirus and Norovirus) and two DNA targets (Adenovirus and Campylobacter jejuni ) were amplified in a quadruplex reaction using Air-Dryable™ Direct RNA/DNA qPCR Stool (MDX141, red ) or mixes from TaqPath™ (Thermo, black ) or UltraPlex™ (QuantaBio, grey ) in the presence of 1.5 mg/mL bile salts. The results illustrate that Air-Dryable™ Direct RNA/DNA qPCR Stool is less affected by increased concentrations of bile, unlike the other mixes.
Sensitive Detection in Multiplex Assays in the Presence of Stool Inhibitors
Lyo-Ready™ Direct DNA qPCR Stool (MDX142)
Escherichia coli O157
Salmonella typhimurium
Campylobacter jejuni
Lyo-Ready™ Direct DNA qPCR Stool | KAPA Probe Force | UltraPlex™
E. coli O157 (Shiga-like/Verotoxin 2 gene), Salmonella typhimurium (16S-23S rRNA gene internal transcribed spacer) and Campylobacter jejuni (16S-23S rRNA gene internal transcribed spacer) were amplified in a triplex reaction using lyophilized Lyo-Ready™ Direct DNA qPCR Stool ( red ) and universal mixes, KAPA Probe Force (Roche, green ) and UltraPlex™ (QuantaBio, grey ) in the presence of 6.6 mg/mL human stool. The results illustrate that Lyo-Ready™ Direct DNA qPCR Stool is not affected by high concentrations of human stool, unlike the other mixes, allowing higher multiplexing capacity and greater reproducibility.
Ideal for Liquid Format or Lyophilized Assays – No Impact on Reaction Efficiency
A) Lyo-Ready ™ Direct DNA qPCR Stool (MDX142)
B) Lyo-Ready ™ Direct RNA/DNA qPCR Stool (MDX143)
10,000 copies 1,000 copies
10,000 copies 1,000 copies
100 copies 10 copies
100 copies 10 copies
lyophilized | liquid
Liquid ( blue ) and lyophilized ( red ) formats of A) Lyo-Ready™ Direct DNA qPCR Stool (MDX142) was used in a 10-fold serial dilution of DNA (10,000, 1,000, 100, and 10 copies respectively) in presence of 20% artificial stool and B) Lyo-Ready™ Direct RNA/DNA qPCR Stool (MDX143) was used in a 10-fold serial dilution of RNA (10,000, 1,000, 100 and 10 copies respectively) in presence of 5% artificial stool. The results illustrate that the lyophilized mixes retain the ability to efficiently amplify to the same level as the liquid mixes with the same level of sensitivity and reproducibility.
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qPCR and RT-qPCR Mixes
Urine-Specific Mixes
Urine
Urine is an ideal clinical specimen because it is excreted in large quantities, non-invasive, and can be self-sampled. Currently, urine specimens are used in the diagnosis and management of infectious diseases (including STDs), hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. However, urine contains substances such as urea and nucleases that can damage DNA or inhibit a PCR reaction. Air-Dryable ™ and Lyo-Ready ™ urine mixes are inhibitor-
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns
Air-Dryable Direct DNA qPCR Urine Mixes
MDX150
Air-Dryable Direct RNA/ DNA qPCR Urine Mixes
MDX151
Lyo-Ready Direct DNA qPCR Urine
MDX152
tolerant and designed for very fast, highly sensitive amplification of DNA and RNA directly from high
Lyo-Ready Direct RNA/DNA qPCR Urine
MDX153
concentrations of urine and are sensitive enough to detect arboviruses, such as Chikungunya virus. Patient urine samples can be used directly on the dried assay, and do not require nucleic acid purification.
Enables highly sensitive detection of cancer markers in urine samples
Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151)
CDC2
IGFBP5
MDK
MDK
(QuantaBio) ™
Air-dried | Ultraplex
Three tumor-related mRNA markers (CDC2 kinase, IGFBP5 and MDK) were amplified from total RNA (from a bladder cancer patient) in a triplex reaction using air-dried Air-Dryable™ Direct RNA/DNA qPCR Urine ( red ) or UltraPlex™ (QuantaBio, grey ) in 10% human urine. The results illustrate that Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151) is able to detect cancer related RNA markers from urine faster and with high sensitivity compared to the other mix.
Superior reaction efficiency with crude urine samples in a multiplex reaction
Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151)
Dengue
Zika
Chikungunya
MDX151 (Air-dried) | Reliance 1-Step Multiplex RT-qPCR Supermix | UltraPlex™ 1-Step ToughMix
Three viral RNA targets (Dengue, Zika and Chikungunya) were amplified in a triplex reaction using air-dried Air-Dryable™ Direct RNA/DNA qPCR Urine (MDX151, red ) and kits from Reliance 1-Step Multiplex RT-qPCR Supermix ( green ) and UltraPlex™ 1-Step ToughMix ( grey ) in the presence of 10% human urine. The results illustrate that dry Air-Dryable Direct RNA/DNA qPCR Urine has higher multiplexing capacity and reproducibility in the presence of 10% urine.
Superior reaction efficiency using crude urine samples in multiplex reactions
Lyo-Ready™ Direct DNA qPCR Urine (MDX152)
Mycoplasma genitalium
Treponema pallidum
Chlamydia trachomatis
Neisseria gonorrheae
MDX152 | Quantabio ToughMix | Roche KAPA | ThermoFisher TaqPath ™
Four sexually transmitted pathogens ( M. genitalium, T. pallidum (Syphilis), C. trachomatis and N. gonorrhoeae ) were amplified in a quadruplex reaction in the presence of 20% human urine using Lyo-Ready ™ Direct DNA qPCR Urine in a lyophilized format ( red ) and compared to Quantabio ToughMix PCR Master Mix ( grey ), Roche KAPA Probe Force qPCR kit ( green ) and ThermoFisher TaqPath ™ ProAmp ™ Multiplex Master Mix ( black ) in a liquid format. The results demonstrate that Lyo-Ready ™ Direct DNA qPCR Urine has higher multiplexing capabilities and better reproducibility than other supplier qPCR mixes.
High amplification sensitivity with low copy templates using either liquid and dried-down assay formats
A) Lyo-Ready Direct DNA qPCR Urine (MDX152)
B) Lyo-Ready Direct RNA/DNA qPCR Urine (MDX153)
10,000 copies
10,000 copies
1,000 copies
1,000 copies
100 copies
100 copies
10 copies
10 copies
Lyophilized | Liquid
Activity of A) Lyo-Ready ™ Direct DNA qPCR Urine in lyophilized ( red ) and liquid ( blue ) format was compared in the ability to amplify a 10-fold serial dilution of synthetic DNA (10,000, 1,000, 100 and 10 copies respectively) and B) Activity of Lyo-Ready ™ Direct RNA/DNA qPCR Urine in lyophilized ( red ) and wet ( blue ) format was compared in the ability to amplify a 10-fold synthetic RNA target (10,000, 1,000, 100 and 10 copies respectively) in the presence of 10% pooled human urine. The results illustrate that the dried mix retains the ability to efficiently amplified to the same level as the wet mix with the same level of sensitivity and reproducibility for both.
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qPCR and RT-qPCR Mixes
Universal Mixes
Universal
Meridian’s Lyo-Ready™ and Air-Dryable™ qPCR and RT-qPCR master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol- free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient- temperature stable assays, or they can be used as a wet mix. They are highly inhibitor-tolerant and ultra-sensitive making them ideal for point-of-care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various qPCR inhibitors and can be used across a wide range of patient sample types.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 1,000 Rxns 50 mL 10,000 Rxns 5 mL 500 Rxns 100 mL 10,000 Rxns 8 mL 1,000 Rxns 100 mL 12,500 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns 10 mL 1,000 Rxns 100 mL 10,000 Rxns
Air-Dryable qPCR Mix, 4x
MDX082
Air-Dryable RT-qPCR Mix, 4x
MDX095
Lyo-Ready qPCR Mix
MDX021
Lyo-Ready qPCR Mix, 2.6x
MDX023
Lyo-Ready 1-Step RT-qPCR Mix
MDX024
Lyo-Ready 1-Step RT-qPCR Virus Mix
MDX062
Full enzyme activity following lyophilization/air-drying
Air-Dryable™ RT-qPCR Mix (MDX095)
Activity of Air-Dryable™ 1-Step RT-qPCR Mix (MDX095) in both wet and air-dried formats were compared on 10-fold dilution mouse RNA template. The air-dried mix showed no loss of activity and sensitivity when compared to freshly prepared wet mixup to the assay limit of detection.
Wet | Air-dried
Retention of reverse transcriptase activity following lyophilization
Lyo-Ready™ 1-Step RT-qPCR Mix (MDX024)
y-actin
GAPDH
B2mg
Primers and probes were added to Lyo-Ready™ 1-Step RT-qPCR Mix (MDX024), dried down and immediately rehydrated ( purple ) and tested against a freshly prepared wet mix ( orange ), in a triplex probe RT-qPCR assay on mouse RNA. The results demonstrate the same reverse transcriptase enzyme activity before and following lyophilization.
Lyophilized | Wet Mix
LAMP & RT-LAMP Mixes
Over the past decade, isothermal amplification techniques such as HCA, MDA and loop-mediated isothermal amplification (LAMP) have played an increasingly important role in point-of-care (POC) diagnostics. LAMP offer several advantages over qPCR and other amplification techniques in terms of sample-to-answer time, sensitivity, specificity, cost and robust amplification at a constant temperature. It is also simpler to operate, using less expensive, portable equipment, making it ideal for field-deployable diagnostics in resource-limited settings. LAMP based assays have been used for numerous applications including the detection of pathogens such as salmonella and malaria, genetically modified crop contamination and in forensics to specifically detect human DNA. In addition, RT-LAMP is widely used for screening for a number of pathogens, particularly RNA viruses, such as influenza, rotavirus, hepatitis C, West Nile fever, Dengue virus, Ebola virus and most recently, SARS-CoV-2. Meridian has developed a blend of enzymes, dNTPs, Mg 2+ , excipients and stabilizers that can be used with customer-selected primers and probes for creating fast, robust DNA or RNA LAMP assays. However, a major factor limiting the accessibility of POC testing is cold chain management which is required to preserve the integrity of the assay reagents. Meridian’s Lyo-Ready ™ and Air-Dryable ™ LAMP Mixes have been designed to overcome this challenge.
Key Features
• Ideal for POC diagnostic or automated high-throughput instruments • Available in universal or Specimen-specific ™ formulations for blood, saliva, urine or stool and suitable for direct detection assays from crude samples
• Fully optimized, concentrated 4x master mixes, just add primers and probe • Contains all the required excipients for subsequent lyophilization or air-drying • Shelf-life greater than 12 months after lyophilization or air-drying
Blood-specific Mixes
Blood
Meridian’s Lyo-Ready ™ Direct DNA and DNA/RNA LAMP Blood Mixes are the newest solution for assays requiring high sensitivity and fast time to results and can detect DNA or RNA down to single copies in blood, serum or plasma samples. The mixes are designed to be extremely inhibitor-tolerant, enabling direct detection using crudely processed samples, making them ideal for developing next-generation, culture-free assays detecting bacterial infections such as sepsis. They outperform other commercial LAMP mixes in terms of sensitivity, specificity and speed which is demonstrated by their quicker time-to- result (TTR) and lower non-specific amplification (NTC) across a range of targets.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Blood Mix
MDX124
Lyo-Ready Direct RNA/ DNA LAMP Blood Mix
MDX125
By using innovative ready-to-use isothermal master mixes such as Lyo-Ready ™ Direct DNA LAMP or RNA/DNA LAMP Blood mix, assay development time for a new ultra-sensitive direct blood assay is rapid, simple and efficient. Perfect for increasing your competitive edge and capturing value faster in the rapidly growing point-of-care market.
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LAMP & RT-LAMP Mixes
Superior Specificity and Quicker Time to Detection for Viral and Bacterial Targets
A) Lyo-Ready™ DNA LAMP Blood (MDX124)
B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
Sample
NTC
HBV
HSV2
EBV
T. vaginalis
M. pneumoniae
HCV
HIV-1
DENGUE 1
WNV
Zika
Lyo-Ready™ Direct DNA LAMP Blood | NEB WarmStart® | Thermo SuperScript™
(A) Lyo-Ready™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for Trichomonas vaginalis , Hepatitis B Virus (HBV), Herpes Simplex Virus 2 (HSV2), Epstein-Barr virus (EBV), Mycoplasma pneumoniae and (B) Lyo-Ready™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Hepatitis C Virus (HCV), HIV-1, Dengue type 1, West Nile Virus (WNV) or Zika. The performance of the mixes were compared against NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/ RNA. Reactions were incubated at 65°C for 60 minutes. Error bars represent the standard deviation across three technical replicates. Both Lyo- Ready™ Direct LAMP Blood mixes delivered faster target amplification (TTR) with higher specificity, as demonstrated by the longest delay in no-template control (NTC) amplification across range of pathogenic DNA and viral RNA targets.
High InhibitorTolerance in the Presence of Whole Blood K2-EDTA or Plasma
(A) Lyo-Ready™ DNA LAMP Blood (MDX124)
(B) Lyo-Ready™ RNA/DNA LAMP Blood (MDX125)
60
60
50
50
40
40
30
30
20
20
10
10
0
0
HBV + 5% Blood
EBV + 20% Blood
P. malaria + 5% Blood
HSV2 + 20% Plasma
M. pneumoniae + 5% Blood
Dengue 1 + 10% Blood
HCV + 10% Blood
WNV + 5% Blood
ZIKV + 5% Blood
HIV-1 Plasma extract
Lyo-Ready™ Direct DNA LAMP Blood | NEB WarmStart® | Thermo SuperScript™
Lyo-Ready™ Direct DNA LAMP Blood (MDX124, red ) was lyophilized with primers for HSV2, EBV, HBV, P. malaria and M. pneumonia and Lyo- Ready™ Direct RNA/DNA LAMP Blood (MDX125, red ) was lyophilized with primers for Dengue Type-1, HCV, WNV, Zika or HIV-1 and has it performance tested against WarmStart® LAMP kit ( orange ) and SuperScript™ IV RT-LAMP Master Mix ( black ). Reactions were prepared in the presence of 5-20% Blood K2-EDTA or plasma extract. The data illustrates better performs with faster amplification across the range of inhibitors for both Lyo-Ready™ Direct LAMP Blood mixes, with earlier TTRs across a broad range of pathogenic targets.
Saliva-Specific Mixes
Saliva
Meridian’s new Lyo-Ready ™ Direct DNA LAMP and RNA/DNA LAMP Saliva mixes are the newest advancement in molecular reagent technology and combine the benefits of a highly inhibitor-tolerant mix, with robust isothermal amplification, to enable direct detection using crudely processed samples. The mixes are uniquely designed to overcome saliva and sputum inhibitors and they do not require a DNA or RNA purification step to and purify a patient sample. In addition, the 4x
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Saliva Mix
MDX134
Lyo-Ready Direct RNA/ DNA LAMP Saliva Mix
MDX135
concentrated master mixes are formulated with lyo-excipients which are optimized for freeze-drying to create ambient- temperature stable assays. By using innovative ready-to-use isothermal master mixes such as Lyo-Ready ™ Direct DNA LAMP or RNA/DNA LAMP Saliva mix, assay development time for a new ultra-sensitive direct saliva assay is rapid, simple and efficient.
Superior assay specificity using samples containing 15% sputum
Lyo-Ready™ Direct DNA LAMP Saliva (MDX134)
Amplification of Mycoplasma pneumoniae genomic DNA (200 copies) was compared using Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) against NEB WarmStart® in samples containing 0% or 15% artificial sputum. Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. No NTCs were detected in this assay. Lyo-Ready™ Direct DNA LAMP Saliva (MDX134) with 0% ( light blue ) and 15% ( dark blue ) artificial sputum demonstrated stronger inhibitor tolerance, retaining its fast TTR in the presence of 15% artificial sputum. In contrast, NEB WarmStart® with 0% artificial sputum ( orange ) amplified slower, as shown by a later TTR, than either reaction using Lyo-Ready™ Direct DNA LAMP Saliva. In addition, in samples containing 15% artificial sputum ( red ), NEB WarmStart® had a delayed amplification of 40 min and the longest TTR of all the reactions.
MDX134 0% sputum | MDX134 15% sputum | NEB WarmStart® 0% sputum | NEB WarmStart® 15% sputum
Maintains consistent performance in samples containing up to 60% saliva
Lyo-Ready ™ Direct RNA/DNA LAMP Saliva (MDX135)
25
Lyo-Ready™ Direct RNA/DNA LAMP Saliva (MDX135) was lyophilized with Mycobacterium tuberculosis (MTB) primers and resuspended with increasing amounts of human saliva containing M. tuberculosis (from 10%- 72%). Reactions were incubated at 65 °C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. The results illustrate that Lyo-Ready™ Direct RNA/ DNA LAMP Saliva maintains a robust performance up 60% saliva and demonstrates consistently late NTCs.
20
15
10
5
0
0% 10%
20%
30%
50%
60%
Saliva (%)
LAMP & RT-LAMP Mixes
Stool-Specific Mixes
Stool
Diarrhoeal disease is the 8th leading cause of death globally and the 2nd leading cause of death in children under the age of five. It is caused by a variety of bacterial, viral and parasitic organisms and is usually spread through the fecal-oral route or by foodborne transmission. Stool has been used a diagnostic specimen to detect the presence of these organisms, and also to diagnose certain cancers and inform of overall gut health. In order to provide the right treatment and prevent serious complications, rapid identification of gastrointestinal infections
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Stool Mix
MDX144
Lyo-Ready Direct RNA/DNA LAMP Stool Mix
MDX145
is crucial. This can be carried out using point-of-care (POC) diagnostic tests that are simple to use, involve minimal-to-no- sample pre-processing, do not require cold room storage and are as sensitive and specific as laboratory-based tests. Assays using stool as a specimen can pose a substantial challenge for POC assays as stool samples can be complex, containing food debris, desquamated epithelial cells and mucus, and potential LAMP inhibitors such as bile salts, polysaccharides, hematin and catabolic substances. Meridian has developed Lyo-Ready ™ Direct DNA LAMP Stool and Lyo-Ready ™ RNA/DNA LAMP Stool mixes to combine high inhibitor tolerance with fast, robust isothermal amplification to enable highly sensitive direct detection using crudely processed stool samples. The mixes contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than two-year’s shelf-life; and are uniquely designed to overcome very high concentrations of inhibitors and so do not require a DNA or RNA purification step to purify a patient sample.
Significantly Higher Tolerance to Inhibitors Found in Crude Stool Material
Lyo-Ready ™ Direct DNA LAMP Stool (MDX144)
The impact of stool inhibitors on LAMP performance was tested for Lyo-Ready™ Direct DNA LAMP Stool (MDX144) in liquid format ( blue ), NEB WarmStart® ( orange ) and Thermo SuperScript™ IV ( black ). Mixes were prepared with primers for Salmonella typhimurium , Adenovirus, Campylobacter jejuni or E. coli O157 in the presence of 0 mg/mL or 6.6 mg/mL human stool and genomic DNA from Salmonella , Campy or E. coli or inactivated adenovirus. All reactions were incubated at 65°C for 60 minutes, and the average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. Results show that MDX144 has the shortest TTR even in the presence of 6.6mg/ml stool, whereas NEB WarmStart® and Thermo SuperScript™ IV have significantly delayed TTRs. The results demonstrate that MDX144 has higher tolerance to inhibitors found in stool compared to other commercial mixes.
Adenovirus
Salmonella typhimurium
60
60
50
50
40
40
30
30
20
20
10
10
0
0
6.6 mg/mL
6.6 mg/mL
0 mg/mL
0 mg/mL
Campylobacter jejuni
Escherichia coli O157
60
60
50
50
40
40
30
30
20
20
10
10
0
0
6.6 mg/mL
6.6 mg/mL
0 mg/mL
0 mg/mL
MDX144 | NEB WarmStart® | Thermo SuperScript ™ IV
Lyo-Ready ™ Direct RNA/DNA LAMP Stool (MDX145)
Rotavirus
RSV virus
Zika virus
60
60
60
50
50
50
40
40
40
30
30
30
20
20
20
10
10
10
0
0
0
0 mg/mL
0 mg/mL
0 mg/mL
12.5 mg/mL
12.5 mg/mL
12.5 mg/mL
Norovirus
Adenovirus
60
60
50
50
40
40
30
30
20
20
10
10
0
0
0 mg/mL
0 mg/mL
12.5 mg/mL
12.5 mg/mL
MDX145 | NEB WarmStart® | Thermo SuperScript™ IV
The impact of stool inhibitors on LAMP performance was tested for Lyo-Ready™ Direct RNA/DNA LAMP Stool (MDX145) liquid format ( blue ), NEB WarmStart® ( orange ) and Thermo SuperScript™ IV ( black ). Mixes were prepared using inactivated viruses (Rotavirus, Norovirus, RSV, Zika (RNA viruses) and Adenovirus (ADV) (DNA virus)), the corresponding inactivated virus and with 0 mg/mL or 12.5 mg/mL stool. All reactions were incubated at 65 °C for 60 minutes, and the average time to results (TTR) was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across six technical replicates. Results show that MDX145 has a shorter TTR than NEB WarmStart® and Thermo SuperScript™ IV in the presence of human stool. MDX145 also demonstrated better performance with faster amplification across all RNA and DNA templates in the presence and absence of human stool. Superior Assay Sensitivity in Challenging Environments such as Low Copy Input and High Bile Salt Concentration
A) Lyo-Ready™ Direct DNA LAMP Stool (MDX144)
B) Lyo-Ready™ Direct RNA/DNA LAMP Stool (MDX145)
Respiratory Syncytial Virus
Salmonella typhimurium
60
60
50
50
40
40
30
30
20
20
10
10
0
0
1000
100
10
1
MDX144 | NEB WarmStart® | Thermo SuperScript™ IV
MDX145 | NEB WarmStart® | Thermo SuperScript™ IV
A) Lyo-Ready™ Direct DNA LAMP Stool (MDX144) liquid format ( blue ), was used for the amplification of 1,000, 100 and 10 and 1 copies of Salmonella DNA in the presence of 3 mg/mL bile salts and B) Lyo-Ready™ Direct RNA/DNA LAMP Stool (MDX145) liquid format ( blue ) was used for the amplification of Respiratory Syncytial Virus (RSV) in the presence of 0 mg/mL, 3 mg/mL and 5 mg/mL bile salts. The results were compared using the same primers and DNA/RNA template for NEB WarmStart® ( orange ) and Thermo SuperScript ( black ). Reactions were incubated at 65°C for 60 minutes, and TTR was measured at 1:10 of end fluorescence. Error bars represent the standard deviation across four technical replicates. The results demonstrate the high sensitivity of Lyo-Ready™ Direct DNA LAMP Stool, even at 1 copy of DNA, and both mixes’ high tolerance to bile salt.
www.meridianbioscience.com/lifescience
LAMP & RT-LAMP Mixes
Urine-specific Mixes
Urine
Urine has long been used as a diagnostic sample type for sexually transmitted infections (STIs) such as chlamydia, gonorrhoea, HSV and HPV, among many others. It has also been used to monitor hormone and metabolic disorders, renal diseases, bladder cancer, urinary tract infections (UTIs) and for monitoring recreational drug use. Urine is an ideal clinical specimen because it is excreted in large quantities, it is non- invasive, and it can be self-sampled. Lyo-Ready ™ Direct DNA LAMP and Direct RNA/DNA LAMP
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Lyo-Ready Direct DNA LAMP Urine Mix
MDX154
Lyo-Ready Direct RNA/DNA LAMP Urine Mix
MDX155
Urine mixes are designed to deliver fast and sensitive amplification of DNA or RNA using direct detection methods. Samples do not require nucleic acid extraction and can be used directly on the assay. The mixes also contain excipients and an optimized buffer system that is compatible with lyophilization to create ambient-temperature stable assays which have more than two-year shelf-life.
Results Delivered in Half the Time with Samples Containing 20-30% Urine
Lyo-Ready™ DNA LAMP Urine (MDX154)
HSV1 + 30% Urine
60
50
40
30
N. gonorrhoea + 30% Urine
20
10
0
N.gonorrhoeae +20% Urine
L. interoggans +30% Urine
M. genitalium +20% Urine
C. trachomatis +30% Urine
T. vaginalis +30% Urine
HSV1 +20% Urine
Lyophilized | NEB WarmStart® | SuperScript™
Lyo-Ready™ Direct DNA LAMP Urine (MDX154, red ) was lyophilized with primers for Neisseria gonorrhoeae, Leptospira interrogans, Mycoplasma genitalium, Chlamydia trachomatis, Trichomonas vaginalis and Herpes Simplex Virus 1 (HSV1) The performance of the mixes was compared against liquid formats of NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/ RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX154 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready™ Direct DNA LAMP Urine (MDX154) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
Results Delivered in Half the Time with Samples Containing 20-30% Urine
Dengue Type-1 + 20% Urine
Lyo-Ready™ RNA/DNA LAMP Urine (MDX155)
Chikungunya + 5% Urine
CHIKV + 5% Urine
HCV + 5% Urine
ZIKV + 20% Urine
WNV + 5% Urine
DENV + 20% Urine
Lyophilized | NEB WarmStart® | SuperScript™
Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155, red ) was lyophilized with primers for either Chikungunya Virus (CHIKV), Hepatitis C Virus (HCV), Zika, West Nile Virus (WNV) or Dengue Type-1. The performance of the mixes was compared against liquid formats of NEB WarmStart® LAMP kit ( orange ) and Thermo SuperScript™ IV RT-LAMP Master Mix ( black ) using the same primers and DNA/RNA. Reactions were prepared in the presence of 5-30% human urine. All reactions were incubated at 65 °C for 60 minutes. Error bars represent the standard deviation across four technical replicates. Results show that MDX155 has earlier TTRs across a broad range of pathogenic DNA and viral RNA targets. The data illustrates Lyo-Ready™ Direct RNA/DNA LAMP Urine (MDX155) performs better and with faster amplification across the range of inhibitors compared to the other mixes.
www.meridianbioscience.com/lifescience
LAMP & RT-LAMP Mixes
Universal Mixes
Universal
Meridian’s Lyo-Ready ™ and Air-Dryable ™ LAMP and RT- LAMP master mixes are the market-leading solution for molecular diagnostic assays requiring fast cycling, superior sensitivity, high inhibitor tolerance and ambient temperature stability. They are 4x concentrated, glycerol-free and contain specialized excipients that are compatible with lyophilization or air-drying to create ambient-temperature stable assays, or they can be used as a wet mix. They are highly inhibitor- tolerant and ultra-sensitive making them ideal for point-of- care or machine-automated multiplexing assays. As universal mixes, they are highly resistant to various LAMP inhibitors and can be used across a range of patient sample types.
PRODUCT
CAT NO. VOLUME REACTIONS
5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns 5 mL 800 Rxns 50 mL 8,000 Rxns
Air-Dryable LAMP Mix
MDX119
Air-Dryable RT-LAMP Mixes
MDX118
Lyo-Ready LAMP Mix
MDX097
Lyo-Ready RT-LAMP 1-Step Mix
MDX108
Fast Polymerization for Quick Sample-to-AnswerTime
Lyo-Ready™ LAMP Mix (MDX097)
The average time-to-result (TTR) for Lyo-Ready ™ LAMP Mix (MDX097, orange ) and NEB WarmStart® ( blue ) were compared for three genes, 9TV10 from Trichomonas Vaginalis (100 copies), hBRCA1 from human genomic DNA (200 copies) and MTB1 from Mycobacterium Tuberculosis (1,000 copies). The results demonstrate the faster speed of Lyo-Ready™ LAMP Mix, with earlier TTR values when compared to NEB WarmStart®. Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence.
25.00
20.00
15.00
10.00
5.00
0.00
9TV10 Trichomonas vaginalis
hBRCA-1
MTB1
Human DNA
Mycobacterium tuberculosis
MDX097 | NEB WarmStart®
Mix performance is not impacted by lyophilization or subsequent room temperature storage
Lyo-Ready™ RT-LAMP 1 Step Mix (MDX108)
LAMP reactions in the presence ( green ) or absence ( teal ) of 10% UTM and sputum were performed in 5 replicates using PMSD4.1 primers and RNA to compare the performance of fresh (wet) Lyo-Ready™ RT-LAMP 1-Step Mix (MDX108) against lyophilized Lyo-Ready™ RT-LAMP 1-Step Mix, stored at 37ºC for 3 weeks. Time-to-result is set as the time at which the fluorescence crossed a threshold of 10% of maximal fluorescence. The data illustrate that the lyophilization process does not affect the stability of the Lyo-Ready™ RT-LAMP Mix and at a storage temperature of 37ºC it can be extrapolated that the lyophilized mix is stable at room temperature for up to 12 months.
30
20
10
0
Wet Mix
Lyophilized
Wet Mix
Lyophilized
No Inhibitor
10% UTM + Sputum
Superior sensitivity with shorter time-to-results (TTR) A) Air-Dryable ™ DNA LAMP (MDX119)
B) Air-Dryable ™ RNA/DNA LAMP (MDX118)
50
80
40
60
30
40
20
20
10
0
0
1000 copies/rxn 100 copies/rxn
20 copies/rxn
NTC
1000 copies/rxn 500 copies/rxn 100 copies/rxn
10 copies/rxn
(A) Conserved regions from the S and P genes of Hepatitis B Virus (1,000, 100 and 20 copies respectively) were amplified using Air-Dryable™ DNA LAMP (MDX119) in an air-dried ( red ) or liquid (blue) format or using NEB WarmStart® ( orange ). (B) Emesvirus (MS2) was amplified using Air-Dryable™ RNA/DNA LAMP (MDX118) in an air-dried ( red ) or liquid ( blue ) format or using NEB WarmStart® ( orange ). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Error bars represent standard deviation over 4 technical replicates. At lower copy numbers, NEB WarmStart® ( orange ) exhibited significantly lower reaction reproducibility compared to Air-Dryable™ DNA LAMP Mix, and a lower TTR was observed for reactions using both Air-Dryable™ DNA LAMP Mix and Air-Dryable™ DNA/RNA LAMP Mix across all dilutions of the Hepatitis B gene and MS2-RNA respectively. The results therefore demonstrate greater sensitivity of Air-Dryable™ LAMP Mixes with shorter TTR across all template concentrations.
High reaction specificity with little-to-no non-specific amplification
Air-Dryable™ RNA/DNA LAMP (MDX118)
Air-Dryable™ RNA/DNA LAMP (MDX118, blue ) was compared to NEB WarmStart® ( orange ) for detection of EBV DNA (1,000 ipc) and MS2 RNA (1,000 ipc). Reactions were incubated at 65°C for 60 min and TTR were measured at 1:10 of end fluorescence. Amplification plots of 3 technical replicates demonstrate Air-Dryable™ RNA/DNA LAMP mix has earlier TTR and no non-specific amplification compared to NEB WarmStart® which has detectable amplification for non-template controls (NTC).
EBV-DNA
MS2-RNA
www.meridianbioscience.com/lifescience
Product List
qPCR & LAMP Master Mixes
• Air-Dryable ™ Direct DNA & DNA/RNA qPCR Blood (MDX092 & MDX121) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Blood (MDX122 & MDX123) • Lyo-Ready ™ Direct DNA & DNA/RNA LAMP Blood (MDX124 & MDX125) Blood • Air-Dryable ™ Direct DNA & DNA/RNA qPCR Saiva (MDX130 & MDX131) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Saliva (MDX132 & MDX133) • Lyo-Ready ™ Direct DNA & DNA/RNA LAMP Saliva (MDX134 & MDX135) Saliva • Air-Dryable ™ Direct DNA & DNA/RNA qPCR Stool (MDX140 & MDX141) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Stool (MDX142 & MDX143) • Lyo-Ready ™ Direct DNA & DNA/RNA LAMP Stool (MDX144 & MDX145) Stool • Air-Dryable ™ Direct DNA & DNA/RNA qPCR Urine (MDX150 & MDX151) • Lyo-Ready ™ Direct DNA & DNA/RNA qPCR Urine (MDX152 & MDX153) • Lyo-Ready ™ Direct DNA & DNA/RNA LAMP Urine (MDX154 & MDX155) Urine • Air-Dryable ™ qPCR & RT-qPCR Mixes (MDX082 & MDX095) • Lyo-Ready ™ qPCR & RT-qPCR Mixes (MDX021 & MDX024) • Air-Dryable ™ DNA & RNA LAMP Mixes (MDX119 & MDX118) • Lyo-Ready ™ DNA & RNA LAMP Mixes (MDX097 & MDX108) Universal
Air-Dryable mixes are a new alternative to lyophilization. Create ambient temperature stable assays using only an oven. What is Air-Dryable?
Learn more
Ordering information: USA 5171 Wilfong Road Memphis, Tennessee 38134 Phone: +1 901-382-8716 Fax: +1 901-333-8223
Email: info@meridianlifescience.com Orders: orders@meridianlifescience.com www.meridianbioscience.com/lifescience
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